Polysaccharides derived from golden mushroom (Cantharellus cibarius Fr.) modulate gut microbiota and enhance intestinal barrier function to ameliorate dextran sulfate sodium-induced colitis in mice DOI Creative Commons

Yamina Alioui,

Hidayat Ullah, Sharafat Ali

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 18, 2024

Introduction Inflammatory bowel disease (IBD), including ulcerative colitis, is marked by intestinal barrier disruptions, immune system dysregulation, and an imbalance in the gut microbiota. The golden chanterelle mushroom, Cantharellus cibarius Fr., a popular edible has shown potential therapeutic benefits. This study examines of crude polysaccharide extract obtained from C. Fr. (CCP) on integrity, inflammatory cytokine levels, microbiota composition murine model colitis induced dextran sulfate sodium (DSS). Methods To induce BALB/c mice were provided to consume autoclaved water with 3% DSS for 7 days, followed 14 days CCP supplementation. assess effects CCP, histological analysis colon tissue was performed, gene expression, responses, tight junction proteins cytokines levels measured analyzed 16S rRNA sequencing evaluated. Results Discussion treatment alleviates symptoms improving body weight, enhancing integrity through increased mucin-2 protein expression. Additionally, administration regulates altered response mitigating expression pro-inflammatory upregulating anti-inflammatory cytokines. Furthermore, supplementation effectively modulates DSS-induced dysbiosis as demonstrated results. These findings suggest that polysaccharides hold promise treating via strengthening barrier, regulating reshaping model. offers novel approach managing chronic condition.

Language: Английский

Lactobacillus johnsonii colonizing in mice intestine contributes to control the gut barrier function via regeneration of the crypt in DSS‐treated mice DOI Creative Commons
Yuying Bai, Mengying Lyu, Takashi Satoh

et al.

JSFA reports, Journal Year: 2024, Volume and Issue: 4(9), P. 336 - 347

Published: Sept. 1, 2024

Abstract Background Unbalanced gut microbiota is considered to cause dysfunction of the barrier function. Lactobacillus johnsonii MG, isolated from mouse feces as associating lactobacilli, enhanced function in Caco‐2 cells via interaction with JAM‐2 tight junctions. In this study, anti‐inflammatory effects L. MG were investigated a colitis model developed by treating mice 3% dextran sulfate sodium (DSS) for 9 days. Results treatment resulted fast recovery body weight and showed significant improvement disease activity index histopathological scores. The histomorphological score increased DSS was significantly improved MG‐treated group. intestine, expression Ocln, Zo1, Itga6, Lama3 , Jam2 genes, which are involved junction functions, upregulated mice. microflora analysis, abundance Ruminococcaceae, Bacteroides Lachnospiraceae reduced DSS‐treated recovered treatment. Conclusion We reported potential regeneration crypts integrity matrix proteins through its association experimental results provide new insights into probiotic effect MG.

Language: Английский

Citations

1
The Promotion of Biotication in Controlled Fermentation Using Kefir Grains and Kombucha Membranes as Starter Cultures DOI
Marina Pihurov, Mihaela Cotârleţ, Gabriela Bahrim

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Through the centuries, fermented foods have demonstrated their bioactivity, usefulness, and sustainability in promoting increase of life quality through multiple actions vivo vitro. Fermented foods, complex biotransformation substrate components, enrich final products with useful biotics, i.e., prebiotics, probiotic microorganisms, metabolites named postbiotics. The artisanal cultures, milk, water kefir grains, kombucha (SCOBY) membranes are functional sustainable microbial symbiotic cultures that offer approachable opportunities for obtaining valuable ingredients. These consortia formed by natural association bacteria (lactic acid acetic bacteria), yeasts, bacteriophages. Various conventional unconventional substrates can be transformed into based on metabolism these microorganisms controlled fermentations targeting improvement biotication, contents pre-, pro-, Thus, biochemical activities from could exploited fermentation conditions to obtain beneficial effects vitro features obtained products.

Language: Английский

Citations

0
Mechanisms of intestinal injury in polychaete Perinereis aibuhitensis caused by low-concentration fluorene pollution: Microbiome and metabonomic analyses DOI
Teng Teng, Yuting Yang, Huihong Li

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 475, P. 134925 - 134925

Published: June 15, 2024

Language: Английский

Citations

0
Polysaccharides derived from golden mushroom (Cantharellus cibarius Fr.) modulate gut microbiota and enhance intestinal barrier function to ameliorate dextran sulfate sodium-induced colitis in mice DOI Creative Commons

Yamina Alioui,

Hidayat Ullah, Sharafat Ali

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 18, 2024

Introduction Inflammatory bowel disease (IBD), including ulcerative colitis, is marked by intestinal barrier disruptions, immune system dysregulation, and an imbalance in the gut microbiota. The golden chanterelle mushroom, Cantharellus cibarius Fr., a popular edible has shown potential therapeutic benefits. This study examines of crude polysaccharide extract obtained from C. Fr. (CCP) on integrity, inflammatory cytokine levels, microbiota composition murine model colitis induced dextran sulfate sodium (DSS). Methods To induce BALB/c mice were provided to consume autoclaved water with 3% DSS for 7 days, followed 14 days CCP supplementation. assess effects CCP, histological analysis colon tissue was performed, gene expression, responses, tight junction proteins cytokines levels measured analyzed 16S rRNA sequencing evaluated. Results Discussion treatment alleviates symptoms improving body weight, enhancing integrity through increased mucin-2 protein expression. Additionally, administration regulates altered response mitigating expression pro-inflammatory upregulating anti-inflammatory cytokines. Furthermore, supplementation effectively modulates DSS-induced dysbiosis as demonstrated results. These findings suggest that polysaccharides hold promise treating via strengthening barrier, regulating reshaping model. offers novel approach managing chronic condition.

Language: Английский

Citations

0