Isolation, characterization, and immunomodulatory activity evaluation of probiotic strains from colostrum and canine milk

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Nov. 23, 2023

Background This study aimed to characterize potential probiotic strains for use in dogs prevent infectious enteropathies. Lactic acid bacteria (LAB) isolated from canine milk and colostrum were characterized according their functional properties, including resistance gastrointestinal conditions, inhibitory effect against pathogens, intestinal adhesion. Methods The immunomodulatory effects of the also analyzed vitro vivo studies. Among evaluated, two LAB (TUCO-16 TUCO-17) showed remarkable pH 3.0, bile salts, pancreatin, as well pathogenic Escherichia coli, Salmonella sp., Clostridium perfringens . Results TUCO-16 TUCO-17 induced a significant increase expression TNF-α, IL-8, TLR2 macrophages. oral administration mice significantly augmented E. coli or infections. Both reduced damage pathogen counts liver spleen avoided dissemination into bloodstream. These protective related ability differentially modulate production IFN-γ, IFN-β, IL-6, KC, MCP-1, IL-10 mucosa. Conclusion strains, TUCO-17, are candidates improving health dogs, particularly inhibit growth Gram-negative pathogens common infections animal's immune response. Further studies required effectively demonstrate beneficial dogs.

Language: Английский

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The Canine Gut Health: The Impact of a New Feed Supplement on Microbiota Composition

Animals, Journal Year: 2024, Volume and Issue: 14(8), P. 1189 - 1189

Published: April 15, 2024

This study aimed to determine the impact of a novel formulation supplement composed natural ingredients, bromelain, quercetin, and Lentinula edodes, on gut microbiota healthy adult dogs. Adult female dogs were administered either placebo (CTR, n = 15) or (TRT, over 28 days. Stool samples collected for 16S rRNA sequencing before administration (T0), at completion (T28), one week after end (T35) characterize changes in microbial communities. QIIME was used both alpha- beta-diversity, ANCOM-BC identify differences taxonomic abundances supplementation. We found significant decrease overall diversity CTR group but no TRT time. Furthermore, we abundance several taxa groups, beneficial bacteria more pronounced group. Specifically, increases sequences belonging genera Bifidobacterium, Lactobacillus, Pediococcus T28 with Bifidobacterium Lactobacillus persisting T35 when compared T0. Importantly, members these are considered important their anti-inflammatory properties, vital fostering balanced robust The results our show potential selectively enhance specific bacterial taxa, offering targeted approach modulating microbiome without causing disruptions equilibrium.

Language: Английский

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Dietary intervention for a growing Australian Kelpie with intestinal lipid malabsorption

Veterinary Record Case Reports, Journal Year: 2024, Volume and Issue: 12(3)

Published: April 23, 2024

Abstract A 12‐week‐old male Australian Kelpie was referred for nutritional consultation after being diagnosed with autosomal recessively inherited intestinal lipid malabsorption (ILM). At the timepoint of referral, dog's bodyweight below ideal growth curve, faecal quality poor and patient received a pancreatic enzyme supplement. The patient's diet at referral based on commercial dry food all age groups contained approximately 20% fat in matter (DM). We formulated highly digestible homemade low‐fat content (10% DM) while meeting recommendations linoleic acid, α‐linolenic eicosapentaenoic acid docosahexaenoic which supported requirements growing dog impaired absorption. adapted according to different phases. To our knowledge, this is first case report highlighting management an ILM. After adaptation, reached his potential.

Language: Английский

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Immunohistochemical studies of the uterine wall in bitches with different states of the reproductive system

BIO Web of Conferences, Journal Year: 2024, Volume and Issue: 114, P. 01017 - 01017

Published: Jan. 1, 2024

Dysfunctions of the reproductive system in bitches are a severe problem. Studies were conducted to understand main differences receptor activity immunocompetent cells different conditions determine functional lymphocyte and progesterone surface receptors. Three groups breeding (a total 15 animals) developed analyze immunohistochemistry characteristics uterus. The immunohistochemical method was used type using monoclonal antibodies. Uterine tissue samples from all showed variable amounts cytotoxic lymphocytes (those with CD8 receptors). Nonetheless, isolated discovered strewn across stroma 2 3. In anaesthetic group, scattered layer mucosa part closer uterine lumen, located singly around excretory parts glands among epitheliocytes. study topography receptors structures studied animals that glandular epithelium had extremely low cell reactivity. All examined clear positive response (progesterone receptors) endothelium endometrial capillaries. established peculiarity distribution uterus depending on state confirms significant role genesis dysfunctions is likely be sensitizing factor.

Language: Английский

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The Microbiome in Critical Illness

Advances in Small Animal Care, Journal Year: 2023, Volume and Issue: 4(1), P. 101 - 112

Published: July 27, 2023

Language: Английский

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