Preliminary Study on the Efficacy of a Recombinant, Subunit SARS-CoV-2 Animal Vaccine against Virulent SARS-CoV-2 Challenge in Cats DOI Creative Commons
Igor Morozov, Natasha N. Gaudreault, Jessie D. Trujillo

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(12), P. 1831 - 1831

Published: Dec. 8, 2023

The objective of this work was to evaluate the safety and efficacy a recombinant, subunit SARS-CoV-2 animal vaccine in cats against virulent challenge. Two groups were immunized with two doses either recombinant spike protein or placebo, administered three weeks apart. Seven after second vaccination, both challenged via intranasal oral routes simultaneously. Animals monitored for 14 days post-infection clinical signs viral shedding before being humanely euthanized evaluated macroscopic microscopic lesions. induced strong serologic responses post-vaccination significantly increased neutralizing antibody post-challenge. A significant difference nasal shedding, reduced virus load (detected using RT-qPCR) observed vaccinates compared mock-vaccinated controls. Duration nasal, oral, rectal also No differences histopathological lesion scores noted between groups. Our findings support protein-based which high levels antibodies indicating that will be efficacious as COVID-19 domestic cats.

Language: Английский

Validation of a plasmonic-based serology biosensor for veterinary diagnosis of COVID-19 in domestic animals DOI Creative Commons

Juliana Fátima Giarola,

Maria Soler, M.‐Carmen Estévez

et al.

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125685 - 125685

Published: Jan. 16, 2024

The coronavirus disease 2019 (COVID-19) pandemic recently demonstrated the devastating impact on public health, economy, and social development of zoonotic infectious diseases, whereby viruses jump from animals to infect humans. Due this potential cross species barrier, surveillance pathogens circulation in domestic close-to-human is indispensable, as they could be reservoirs. Optical biosensors, mainly those based Surface Plasmon Resonance (SPR), have widely its ability for providing direct, label-free, quantitative bioanalysis with excellent sensitivity reliability. This biosensor technology can provide a powerful tool veterinary field, potentially being helpful monitoring infection spread. We implemented multi-target COVID-19 serology plasmonic rapid testing screening common European animals. serological assay enables detection total SARS-CoV-2 antibodies (IgG + IgM) generated towards both S N viral antigens. analysis performed less than 15 min low-volume serum sample (<20 μL, 1:10 dilution), reaching limit 49.6 ng mL

Language: Английский

Citations

4
Evidence of SARS‐CoV‐2 Exposure in Cats and Dogs From Households in Romania and Long‐Term Specific Seroconversion in Cats DOI Creative Commons
Luanda Elena OȘLOBANU, Luciana Alexandra Crivei, Gheorghe Săvuţă

et al.

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(3)

Published: April 28, 2025

ABSTRACT Introduction The COVID‐19 pandemic underscored the need to understand zoonotic transmission of pathogens. SARS‐CoV‐2 has been reported be transmitted from humans pets, including cats and dogs, particularly after close contact with infected individuals. Studies have shown that are more susceptible natural infection can transmit virus other humans. route represents a risk for animal health workers. Despite global reports, data Romania remain sparse. This study aimed evaluate presence specific antibodies in household dogs during pandemic. Methods was conducted at veterinary clinic Iasi City, Romania, March 2020 December 2022. Blood samples were collected 84 82 had COVID‐19‐positive owners. Plasma tested anti‐SARS‐CoV‐2 using an ELISA test, followed by confirmation seroneutralisation (SN) assay. SN assay used MAD6 strain Omicron determine neutralising antibody titers. Results testing showed seroprevalence 9.5% 11% dogs. Further confirmed 9.4% 2.4% One cat maintained over year, though reduced Most cats' did not cross‐react strain, indicating limited cross‐reactivity. highlighted higher titers compared likely due efficient viral replication cats. Conclusions provides first serological evidence exposure pets findings suggesting their owners than living households one area city prone high levels seroconversion detected. These results align demonstrating when

Language: Английский

Citations

0
Assessment of the Risk Impact of SARS-CoV-2 Infection Prevalence between Cats and Dogs in America and Europe: A Systematic Review and Meta-Analysis DOI Creative Commons
Marcos Jessé Abrahão Silva, Davi Silva Santana,

Marceli Batista Martins Lima

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(4), P. 314 - 314

Published: April 12, 2024

The COVID-19 pandemic represented a huge obstacle for public health and demonstrated weaknesses in surveillance promotion systems around the world. Its etiological agent, SARS-CoV-2, of zoonotic origin, has been target several studies related to control prevention outbreaks epidemics not only humans but also animals. Domestic animals, such as dogs cats, have extensive contact with can acquire infection both naturally directly from humans. objective this article was summarize seroprevalence findings SARS-CoV-2 cats correlate them strength risk between each them. This is systematic review meta-analysis following recommendations PRISMA 2020. search selection papers carried out using vivo experimental works animals descriptors (MeSH/DeCS) “Animal”, “Public Health”, “SARS-CoV-2” “Pandemic” (together AND) English, Portuguese or Spanish Science Direct, PUBMED, LILACS SciELO databases. ARRIVE checklist used methodological evaluation Comprehensive Meta-Analysis v2.2 software Difference Risk (RD) test evaluate statistical inferences (with subgroups by continent). Cats showed greater susceptibility compared joint analysis (RD = 0.017; 95% CI 0.008–0.025; p < 0.0001) American subgroup 0.053; 0.032–0.073; 0.0001), unlike lack significant difference on European continent 0.009; −0.001–0.018; 0.066). Therefore, it observed that interest due set biological ecological aspects these there are factors influence spread possible spillover events virus thanks anthropozoonotic context.

Language: Английский

Citations

2
Investigation of SARS-CoV-2 Infection among Companion Animals in Households with Confirmed Human COVID-19 Cases DOI Creative Commons
Heather Venkat, Hayley D. Yaglom,

Gavriella Hecht

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 466 - 466

Published: June 1, 2024

We aimed to characterize SARS-CoV-2 infection in companion animals living households with COVID-19-positive people and understand the dynamics surrounding how these become infected. Public health investigators contacted at least one confirmed, symptomatic person COVID-19 for study recruitment. Blood, nasal, rectal swab specimens were collected from pet dogs cats a questionnaire was completed. Specimens tested by RT-PCR, neutralizing antibodies; genomic sequencing performed on viral-positive samples. A total of 36.4% 110 pets enrolled had evidence SARS-CoV-2. Pets more likely test positive if immunocompromised, than home COVID-19. Among 12 multi-pet where positive, 10 other positive. Whole-genome revealed genomes viral lineages circulating community during time sample collection. Our findings suggest high likelihood transmission multiple when very close interactions humans. Further surveillance studies are needed new variants impact opportunities spillover susceptible species.

Language: Английский

Citations

2
Evidence of SARS-CoV-2 infection and co-infections in stray cats in Brazil DOI
Anisleidy Pérez Castillo, João Victor Oliveira Miranda, Paula Luize Camargos Fonseca

et al.

Acta Tropica, Journal Year: 2023, Volume and Issue: 249, P. 107056 - 107056

Published: Oct. 30, 2023

Language: Английский

Citations

4
A Comprehensive Study of Cellular and Humoral Immunity in Dogs Naturally Exposed to SARS-CoV-2 DOI Creative Commons
Beatriz Davinia Tomeo-Martín, Pablo Delgado-Bonet, Teresa Cejalvo

et al.

Transboundary and Emerging Diseases, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) was identified as the causal agent behind disease 2019 (COVID‐19), a declared pandemic in 2020. Because of zoonotic origin SARS‐CoV‐2 and close contact kept by domestic dogs with their owners, it became imperative to understand role epidemiology virus transmission. In present study, we determined presence described long‐term immune effects 24 exposed environment. Our findings highlight that only subset dogs, naturally SARS‐CoV‐2, exhibit humoral response new (close 17% had IgM antibodies 33% has IgG antibodies). We for first time SARS‐CoV‐2‐specific IFN‐ γ ‐secreting cells (approximately half our dogs). While 56% maintained 8 months, 22% cellular after 4 months. Although some alterations blood parameters proinflammatory cytokines were described, there no evidence indicating an exacerbated cytokine release process. Considering none animals enrolled this study showed viral shedding presented specific responses, is reasonable propose canine system certain companion effective at blocking negative replication, thereby suggesting would not be potential transmitters pathogen other or species could aid promoting collective immunity.

Language: Английский

Citations

1
Editorial: High-impact respiratory RNA virus diseases DOI Creative Commons
Víctor M. Petrone-García, Inkar Castellanos-Huerta, Guillermo Téllez–Isaías

et al.

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Aug. 22, 2023

EDITORIAL article Front. Vet. Sci., 22 August 2023Sec. Veterinary Infectious Diseases Volume 10 - 2023 | https://doi.org/10.3389/fvets.2023.1273650

Language: Английский

Citations

2
Validation of a Plasmonic-Based Serology Biosensor for Veterinary Diagnosis of Covid-19 in Domestic Animals DOI
Juliana de Fátima Giarola, Maria Soler, M.‐Carmen Estévez

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
SARS-CoV-2 exposure in hunting and stray dogs of southern Italy DOI Creative Commons
Gianmarco Ferrara, Ugo Pagnini, Serena Montagnaro

et al.

Veterinary Research Communications, Journal Year: 2024, Volume and Issue: 48(6), P. 4037 - 4042

Published: Aug. 21, 2024

Abstract Evidence of exposure to the pandemic SARS-CoV-2 has been described in numerous animal species, including pets, which are predisposed coming into contact with this virus due their close relationship owners. It accepted that dogs poorly susceptible and seroconversion, rather than shedding, occurs following infection, can occur directly through infected owners or indirectly environmental contamination. In study, seroprevalence was evaluated apparently health hunting stray Campania region, southern Italy (sampled September 2023). A total 5/112 (4.5%) animals tested seropositive using two different commercial ELISAs. Stray had greater dogs. The feces blood each were a real-time PCR targeting nucleocapsid ORF1ab coding sequences. No positive molecular investigations, indicating past without active infection at time sampling.

Language: Английский

Citations

0
Higher Frequency of SARS-CoV-2 RNA Shedding by Cats than Dogs in Households with Owners Recently Diagnosed with COVID-19 DOI Creative Commons
Michele Lunardi, Felippe Danyel Cardoso Martins, Emanuele Gustani‐Buss

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(10), P. 1599 - 1599

Published: Oct. 11, 2024

Studies have demonstrated the susceptibility of companion animals to natural infection with SARS-CoV-2. Using quantitative reverse transcription polymerase chain reaction and sequencing analyses, this study investigated SARS-CoV-2 RNA excretion in pets households infected owners. Oropharyngeal rectal swabs were collected from dogs cats Parana, Southern Brazil, between October 2020 April 2021. Viral was detected 25% 0.98% dog oropharyngeal swabs; however, systemic, respiratory, gastrointestinal signs absent. Complete viral genomes belonged Gamma lineage. Phylogenetic analyses indicated that pet samples probably derived human-positive cases Parana. oropharynx more frequent than dogs. Mutations S protein characteristic strains present all sequenced strains. The receptor-binding domain these Brazilian did not show any additional mutations reported NSP6, NSP12, N proteins previously mapped infect deer or minks detected. This highlights importance actively monitoring continued exposure. Monitoring genetic changes is crucial because new variants adapted may pose human health risks.

Language: Английский

Citations

0