Combined Analysis of Second- and Third-Generation Transcriptome Sequencing for Gene Characteristics and Identification of Key Splicing Variants in Wound Healing of Ganxi Goat Skin DOI Creative Commons
Yang Xue, Lucheng Zheng, Junhong Huo

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(21), P. 3085 - 3085

Published: Oct. 26, 2024

Ganxi goat is a local breed of unique to Jiangxi Province, China, named for its primary distribution in the region. goats are primarily raised through grazing, showing good adaptation humid and hot climate, strong disease resistance, occupying an important position livestock industry. The skin, as main barrier body, plays indispensable role resisting invasion external pathogenic factors has received increasing attention medical scientific fields. In this study, skin was used research subject. Full-length transcriptome sequencing performed using PacBio third-generation technology supplement improve annotation information genome. A combined analysis second- analyze splicing variant events hub genes (CDC20, MMP2, TIMP1, EDN1) expression changes each samples on day 0 5 after surgical wounding. regulatory related gene variants wound healing analyzed. total 926,667 full-length non-chimeric sequences were obtained, optimizing 3794 genomic loci identifying 2834 new genes, 256 LncRNAs, 12,283 alternative events, 549 with polyadenylation, 112 fusion genes. Three forms identified both CDC20 EDN1 seven two TIMP1. levels most showed significant days wounding, potentially participating regulation healing. This study provides fundamental data genome offers reference studying mechanisms

Language: Английский

Bioinformatics-based Identification of Ferroptosis-related Genes and their Diagnostic Value in Gestational Diabetes Mellitus DOI
Xiaomei Lv,

Yujun An

Endocrine Metabolic & Immune Disorders - Drug Targets, Journal Year: 2024, Volume and Issue: 24(14), P. 1611 - 1621

Published: Feb. 13, 2024

Background: Gestational diabetes mellitus (GDM) is considered a risk factor for heart metabolic disorder in future mothers and offspring. Ferroptosis new type of programmed cell death, which may participate the occurrence development GDM. Objective: This study aims to identify ferroptosis-related genes GDM by bioinformatics methods explore their clinical diagnostic value. Methods: The dataset GSE103552 was analyzed using Gene Expression Omnibus (GEO) database screen differentially expressed (DEGs) Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis proteinprotein interaction (PPI) network were performed. sets ferroptosis retrieved MSigDB GSVA gene set performed on database. Finally, logistic regression differentiate between patients controls markers. Results: A total 179 DEGs identified expression profile GO KEGG revealed significant TGF-β, p53 signaling pathway, platelet activation, glutathione metabolism, sensory perception taste, leukocyte vascular endothelial migration regulation. (n = 107) associated with screened analysis. disease scores intersected 35 identified. PPI two key as CCNB2 CDK1. Wilcox-test showed low CDK1 area under ROC curve (AUC) prognostic model 0.822. Conclusion: CDK1, can be used valid indicators diagnosis

Language: Английский

Citations

2
Comprehensive analysis of cuproptosis-related ceRNA network and immune infiltration in diabetic kidney disease DOI Creative Commons
Fang Lan,

Jie Zhao,

Dan Liang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e35700 - e35700

Published: Aug. 1, 2024

Diabetic kidney disease (DKD) is the primary contributor to renal failure and poses a severe threat human health. Accumulating studies demonstrated that competing endogenous RNA (ceRNA) network involved in cuproptosis DKD progression. However, role of cuproptosis-associated ceRNA immune infiltration remains largely unclear. This study aimed investigate cuproptosis-related regulation DKD.

Language: Английский

Citations

1
Comprehensive Analysis of Cuproptosis-Related Cerna Network and Immune Infiltration in Diabetic Kidney Disease DOI
Fang Lan,

Jie Zhao,

Dan Liang

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Combined Analysis of Second- and Third-Generation Transcriptome Sequencing for Gene Characteristics and Identification of Key Splicing Variants in Wound Healing of Ganxi Goat Skin DOI Creative Commons
Yang Xue, Lucheng Zheng, Junhong Huo

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(21), P. 3085 - 3085

Published: Oct. 26, 2024

Ganxi goat is a local breed of unique to Jiangxi Province, China, named for its primary distribution in the region. goats are primarily raised through grazing, showing good adaptation humid and hot climate, strong disease resistance, occupying an important position livestock industry. The skin, as main barrier body, plays indispensable role resisting invasion external pathogenic factors has received increasing attention medical scientific fields. In this study, skin was used research subject. Full-length transcriptome sequencing performed using PacBio third-generation technology supplement improve annotation information genome. A combined analysis second- analyze splicing variant events hub genes (CDC20, MMP2, TIMP1, EDN1) expression changes each samples on day 0 5 after surgical wounding. regulatory related gene variants wound healing analyzed. total 926,667 full-length non-chimeric sequences were obtained, optimizing 3794 genomic loci identifying 2834 new genes, 256 LncRNAs, 12,283 alternative events, 549 with polyadenylation, 112 fusion genes. Three forms identified both CDC20 EDN1 seven two TIMP1. levels most showed significant days wounding, potentially participating regulation healing. This study provides fundamental data genome offers reference studying mechanisms

Language: Английский

Citations

0