Advancing toxicity studies of per- and poly-fluoroalkyl substances (pfass) through machine learning: Models, mechanisms, and future directions

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174201 - 174201

Published: June 25, 2024

Language: Английский

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Screening of Estrogen Receptor Activity of Per- and Polyfluoroalkyl Substances Based on Deep Learning and In Vivo Assessment

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125843 - 125843

Published: Feb. 1, 2025

Language: Английский

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60 Years of the 3Rs symposium: Lessons learned and the road ahead

ALTEX, Journal Year: 2024, Volume and Issue: 41(2), P. 179 - 201

Published: Jan. 1, 2024

When The Principles of Humane Experimental Technique was published in 1959, authors William Russell and Rex Burch had a modest goal: to make researchers think about what they were doing the laboratory – do it more humanely. Sixty years later, their groundbreaking book celebrated for inspiring revolution science launching new field: 3Rs alternatives animal experimentation. On November 22, 2019, some pioneering leading scientists field gathered at Johns Hopkins Bloomberg School Public Health Bal­timore 60 Years Symposium: Lessons Learned Road Ahead. event sponsored by Center Alternatives Animal Testing (CAAT), Foundation Chemistry Research Initiatives, Alternative & Development (ARDF), American Cleaning Institute (ACI), International Fragrance Association (IFRA), In Vitro Sciences (IIVS), John “Jack” R. Fowle III, Society Toxicology (SoT). Fourteen pres­entations shared history behind publication, international efforts achieve its aims, stumbling blocks progress, as well remarkable achievements. day tribute Burch, testament is possible when people from many walks life science, government, industry work toward common goal.

Language: Английский

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The New Paradigm in Animal Testing – “3Rs Alternatives”

Regulatory Toxicology and Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 105705 - 105705

Published: Sept. 1, 2024

Language: Английский

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Premises for a digital twin of the Atlantic salmon in its world: Agency, robustness, subjectivity and prediction

Aquaculture Fish and Fisheries, Journal Year: 2024, Volume and Issue: 4(1)

Published: Feb. 1, 2024

Abstract Aquaculture of Atlantic salmon Salmo salar is in transition to precision fish farming and digitalization. As it easier, cheaper safer study a digital replica than the system itself, model can potentially improve monitoring prediction facilities operations replace live many what‐if experiments. Regulators, consumers voters also want insight into how like be aquaculture. However, such information credible only if natural physiology behaviour living adequately represented. To able predict unfamiliar, confusing stressful situations, modeller must aim for sufficiently realistic behavioural based on animal's proximate robustness mechanisms. We review knowledge status algorithms evolution has formed control decisions set priorities ontogeny. Teleost body through genes, hormones, nerves, muscles, sensing, cognition behaviour, latter being agentic, predictive subjective, man‐made environment. These are challenges when constructing salmon. This perspective useful modelling other domesticated wild animals Anthropocene environments.

Language: Английский

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Young TPI: empowering animal-free science among the next- generation of scientists

Frontiers in Toxicology, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 15, 2025

Strategies emphasizing animal-free innovation are imperative for the contemporary and future scientific research. They not only address important ethical concerns, but also should directly improve research accuracy reliability through redirecting inquiry toward more reliable translatable methodologies. Promotion encouragement use of innovations among next-generation scientists, alongside knowledge acquisition training in increased capabilities novel technologies, fundamental advancing science welfare animals used purposes. The Dutch government has promoted initiatives such as Transitie Proefdiervrije Innovatie (TPI) to make public aware current situation. However, transition towards will span over than two generations. In this context, Young TPI emerged the-first-of-its-kind network comprising young professionals students dedicated revolutionizing practices by catalyzing shift Grounded on three pillars - collaboration, awareness-raising, networking evolved into a premier youth Netherlands. Boasting 270 members spanning 49 institutions, including biotechnology startups pharmaceutical companies universities, harnesses diverse expertise its propel sustainable, future-proof promote continuous dialogue with wide range stakeholders. This manuscript describes conception, establishment, progress from start present, detailing strategy communication, activities, funding mechanisms, ongoing endeavors enlist new forge strategic alliances pursuit mission.

Language: Английский

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Premises for digital twins reporting on Atlantic salmon wellbeing

Behavioural Processes, Journal Year: 2025, Volume and Issue: 226, P. 105163 - 105163

Published: Feb. 4, 2025

Many species of fish, birds and mammals commonly live in human captivity; Atlantic salmon Salmo salar is one them. The international legal status the welfare captive animals slowly developing still requires rigorous specification. For example, even though fish have complex cognition elements sentience, United Nations' animal principles take a functional health-centred perspective overlooking cognitive-affective component. Wellbeing problems remain major source slow growth high mortality intensive aquaculture salmon. value system for decision making vertebrates based on expectations emotional wellbeing options available linked with individual's assessment its future. We propose new approach monitoring improving (or any other or wild vertebrate) modelling salmon's by digital twins, which are simulation models that implement bodily mechanisms organism. Indeed, predictions boredom, stress can all be captured computational evolutionary model factors underlying behaviour. explain how such an agent-based twins constructed subjective experience along prediction near future allostasis (the preparation expected future). attempt to identify building blocks required twin deliver early warnings about escalating issues could eventually lead negative effects health aquaculture. These would provide critical insights optimizing production processes significantly reduce reliance experiments. Overall, reports population support implementation 3Rs - replacement, reduction, refinement offering actionable information farmers as well consumers, voters, politicians regulators relevant guide experimental work across species.

Language: Английский

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Ethical and academic dilemmas in authorship of clinical research publications: a medical oncologist’s perspective

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(3)

Published: Feb. 11, 2025

Language: Английский

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Investigating Connectivity Deficits in Alzheimer’s Disease Using a Novel 3D Bioprinted Model Designed to Quantify Neurite Outgrowth

Bioengineering, Journal Year: 2025, Volume and Issue: 12(3), P. 245 - 245

Published: Feb. 28, 2025

Here, we present a novel 3D bioprinted model of the forebrain cortex designed to quantify neurite outgrowth across hydrogel bridge. To validate this model, cultured Alzheimer’s disease (AD) cortical populations derived from human iPSCs carrying APP (amyloid precursor protein) mutations (K670M/N671L + V717F). Neurite and synapse formation were significantly impaired in AD mutant cultures compared controls, but was not replicated 2D, highlighting deficits these traditional 2D cell culture models. investigate mechanisms underlying models AD, assessed amyloid-β dysfunction, mitochondrial health, oxidative stress both conditions. In exhibited reduced membrane potential fragmented networks, indicating dysfunction cellular energy deficits. Additionally, elevated proteostasis disruption identified as indicators damage, which may be limiting extension. Furthermore, transcriptomic (bulk RNA-Seq) analysis revealed distinct differences gene expression pathways between suggesting alternate pathology This study demonstrates functionality for quantifying connectivity identifying mechanisms.

Language: Английский

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Accelerating Animal Replacement: How Universities Can Lead — Results of a One-Day Expert Workshop in Zurich, Switzerland

Alternatives to Laboratory Animals, Journal Year: 2025, Volume and Issue: 53(2), P. 106 - 118

Published: March 1, 2025

This report is a result of an interdisciplinary workshop held at the Collegium Helveticum in Zurich, Switzerland February 2024, which ideas for accelerating NAMs (New Approach Methodologies) Swiss universities were shared and discussed. Due to regional differences university organisation funding structures, not all recommendations will be transferable regions worldwide. All participants qualified contribute discussion, due their knowledge experience Three Rs, particular with regard implementation. The believed that universities, play pioneering role so many other areas, should also exploit innovative potential field animal-free research. uncovered four areas would need addressed order achieve significant change science culture do more justice namely: language — framing (pro-replacement official statements); transfer communicating findings teaching (redirecting curriculum); values within faculties; structured implementation well-coordinated planning transformation (establishment ‘transition unit’). Specific strategies implementing these are outlined. In addition, we discuss why replacement animal testing essential goal this has yet been achieved, concerted efforts toward required.

Language: Английский

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