Evaluation of the effectiveness of the use of exosomes in the regulation of the mitochondrial membrane potential of frozen/thawed spermatozoa
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(7), P. e0303479 - e0303479
Published: July 3, 2024
Numerous
studies
confirm
the
involvement
of
extracellular
vesicles
(EVs)
in
regulation
physiological
processes
mammalian
sperm
cells.
It
has
been
proven
that
they
take
part
capacitation,
acrosonmal
reaction,
and
anti-oxidation.
Despite
growing
interest
biomedical
potential
(including
search
for
new
reproductive
biomarkers)
EVs,
role
seminal
maintaining
semen
quality
during
cryopreservation
not
yet
established.
Therefore,
objective
this
experiment
was
to
evaluate
effectiveness
use
mitochondrial
membrane
bovine
explain
mechanisms
EV
action
cell
cryopreservation.
Exosomes
were
isolated
from
bull
plasma,
measured,
used
extender
supplementation.
Semen
samples
collected
Simmental
bulls,
diluted,
pre-evaluated.
Then
divided
into
equal
fractions
did
contain
EVs
or
supplemented
with
0.75;
1.5
2.25
mg/ml
EVs.
The
test
frozen/thawed
potential,
DNA
integrity,
viability
evaluated.
have
established
a
positive
effect
on
cryopreserved
structures.
most
favourable
level
mg
/
ml,
which
can
be
successfully
improve
cryostability
freezing/thawing.
In
study,
exosomes
plasma
concentrated
dose
freezing
shown
significantly
cells
by
supporting
potentials
protecting
cytoplasmic
spermatozoa.
Language: Английский
Mitochondria-targeted antioxidant MitoQ improves the quality of low temperature-preserved yak semen via alleviating oxidative stress
Yanjin Zhu,
No information about this author
Jun Yu,
No information about this author
Qinhui Yang
No information about this author
et al.
Animal Reproduction Science,
Journal Year:
2024,
Volume and Issue:
273, P. 107680 - 107680
Published: Dec. 19, 2024
Language: Английский
Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis
Anel Umirbaeva,
No information about this author
Andrey Kurenkov,
No information about this author
Aizhan Makhanbetova
No information about this author
et al.
Frontiers in Veterinary Science,
Journal Year:
2024,
Volume and Issue:
11
Published: April 24, 2024
Cryopreservation
of
sperm
is
an
essential
technique
in
assisted
reproduction
cattle.
The
objective
the
study
was
to
systematically
review
and
synthesize
literature
on
bull
semen
quality
evaluation
based
comparison
morphological
metabolic
parameters
cryopreserved
bovine
spermatozoa
such
as
DNA
integrity,
mitochondrial
status,
plasma
membrane
alterations,
total
motility,
morphology
(%
abnormal
cells).
electronic
databases
PubMed,
Web
Sciences,
Scopus,
Google
Scholar
were
searched
up
December
2023.
Studies
references
included
if
they
reported
following
parameters:
aberrations
cells)
for
conventional
spermatozoa.
After
search,
out
1,526
original
studies,
only
40
meta-analysis.
Standardized
mean
differences
(SMD)
with
95%
confidence
intervals
estimated
chosen
a
meta-analysis
performed
using
random
effects
model.
tau-squared
(tau
Language: Английский