Macrophage extracellular traps are induced by Mycoplasma bovis in bovine macrophages through NADPH oxidase/ROS‐dependent manner and their antibacterial efficacy DOI Creative Commons
Ahmed Adel Baz, Shengli Chen,

Huafang Hao

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 9, 2024

Mycoplasma bovis has emerged as a significant pathogen in cattle, leading to considerable economic losses the cattle industry. It is associated with various clinical syndromes, including pneumoniae, mastitis, and arthritis. The innate immune response, particularly macrophages, plays crucial role combating infections caused by such pathogens. release of macrophage extracellular traps (METs) represents novel defense mechanism employed macrophages; however, impact M. on formation METs bovine macrophages remains unknown. Therefore, primary objective this study investigate which affects evaluate their bactericidal efficacy. Our findings revealed that cell lines released DNA fibrils colocalized histones cellular proteins response infection, visualized confocal scanning electron microscopy. Moreover, was found depend NADPH oxidase, for reactive oxygen species generation. Importantly, led lysis indicated lactate dehydrogenase, process be independent apoptosis pyroptosis. infection effective both killing restricting its growth. Overall, our first described how were produced responded demonstrated function bacterial killing, helpful improve understanding host's against pathogen.

Language: Английский

The Pathogenomics of the Respiratory Mycoplasma bovis Strains Circulating in Cattle Around the Texas Panhandle, USA DOI Creative Commons
Ethan Dudley, Matthew A. Scott,

Hatem Kittana

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(2), P. 167 - 167

Published: Feb. 8, 2025

Bovine respiratory disease (BRD) is a major economic and animal welfare issue in the beef industry. Mycoplasma bovis one of main causal organisms, particularly chronic cases. Due to difficulty isolating M. from clinical isolates, there lack information on genetic diversity this pathogen Texas panhandle region United States. Therefore, our objective was provide genome-level characterization isolated lung lesions dairy cattle panhandle. Fifty-four isolates displaying mycoplasma-like growth were recovered bovine by Veterinary Medical Diagnostic Laboratory 2021 2022. Of these 32 determined be via species-specific qPCR using uvrC gene. Long-read whole-genome sequencing used identify key virulence factors, antimicrobial resistance genes, assess isolates. Fisher’s exact tests associations between isolate characteristics host metadata, including state origin, type operation, age, sex. Our results indicate that considerable among despite their shared geography panhandle, though significant clustering based metadata observed. Analysis pangenome showed study also harbor diverse array but no genes identified study.

Language: Английский

Citations

0
Alteration in Tracheal Morphology and Transcriptomic Features in Calves After Infection with Mycoplasma bovis DOI Creative Commons
Fan Liu, Fei Yang,

Lei Guo

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 442 - 442

Published: Feb. 18, 2025

Mycoplasma bovis is one of the most important pathogens in animal husbandry, and current infection morbidity rates are increasing year by year, causing great losses to farming industry seriously affecting welfare. In this study, we took tracheal tissues from calves infected with M. make pathological tissue sections for observation, selected transcriptome sequencing screen differentially expressed genes based on threshold |log2FoldChange| > 1 Padjust < 0.05 functional enrichment, explore depth potential mechanisms bovine damage caused tracheitis. Experiments were conducted observe changes after through trachea bovis-infected calves. From results, mined main differential metabolic pathways It was found that cricoid cartilage congested hemorrhagic calves, showed localized necrosis epithelial cells, disorganization, high inflammatory cell infiltration interepithelial lamina propria, some detachment. Transcriptome identified 4199 DEGs, including 1378 up-regulated 2821 down-regulated genes. KEGG enrichment analysis indicated enriched 59 significantly differing signaling pathways, a number related tracheitis induced unearthed. The major ones included IL-17, Toll-like receptor, JAK/STAT, PI3K-Akt pathway, etc. trachea, results also significant differences expression key such as IL-6 factor, CASP8, APOA1.

Language: Английский

Citations

0
Major Causative Bacteria of Dairy Cow Mastitis in the Inner Mongolia Autonomous Region, China, 2015–2024: An Epidemiologic Survey and Analysis DOI Creative Commons
Hongmei Zhao, Ting Guo, Yaping Zhou

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 197 - 197

Published: Feb. 21, 2025

In this study, we sought to evaluate the prevalence of bacterial pathogens mastitis in dairy cattle Inner Mongolia Autonomous Region, China. The study was conducted from 2015 2024 using a total 12,053 clinical (CM) and sub-clinical (SCM) samples. were isolated identified by standard bacteriological mycological methods. most common Escherichia coli (13.82%), Staphylococcus aureus (10.28%), Klebsiella spp. (8.96%), Streptococcus agalactiae (7.45%), uberis (6.60%), coagulase-negative staphylococci (5.84%), dysgalactiae (4.21%). From 2017, primary responsible for causing cows agalactiae. 2018, frequently pathogen aureus. Notably, isolation rate increased 12.31% 21.72%, 7.52% 14.01% 2019–2024. Mycoplasma only detected cases, with separation as high 6.95%. summary, environmental is gradually increasing, while that contagious has been continuously declining. This indicates current prevention strategies infectious are effective. As next step, it will be important develop new specifically targeting pathogenic microorganisms.

Language: Английский

Citations

0
Mycoplasma bovis : A review of vaccination and diagnostic initiatives DOI

Isaac Dayo Olorunshola,

Kabiru Ahmad, A.R. Peters

et al.

CABI Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Mycoplasma bovis infections in cattle constitute a worldwide problem with significant detrimental economic impacts on industry. Mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disorders are its clinical manifestations. Presently, no vaccines commercially available; antimicrobial resistance is increasing; diagnostic sensitivity testing needs to be improved; new rapid diagnosis kits eminent for implementation of treatment antimicrobials. We conducted systematic search databases such as PubMed, Scopus, Web Science, Google Scholar, AGRIS African Journals Online (AJOL), from 1995 2024. Searched keywords, as, bovis, M : vaccine development, techniques strain variability using the predefined criteria were used address review objectives. Although they have preventative function, – killed, live attenuated, subunit types face difficulties because M. strains vary widely. Every approach has own set benefits drawbacks, those that been studies include conventional culture identification, serological testing, immunohistochemical demonstration tissues, sophisticated molecular like PCR, qPCR, next-generation sequencing. For early detection, successful treatment, vaccination efficacy monitoring, accurate crucial. Future directions managing -associated diseases improving accessibility creating broad-spectrum vaccinations. By incorporating these developments, it may possible enhance health cattle, promote sustainability livestock production, increase food security. This points urgent need further research innovation advancement support

Language: Английский

Citations

0
Validation of a rapid on-farm culture system for group classification of clinical mastitis-causing pathogens DOI Creative Commons

Fernando J. Guardado Servellon,

D.L. Renaud, Bruno Joaquin Paredes Osorio

et al.

JDS Communications, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Integrating the Transcriptome and Proteome to Postulate That TpiA and Pyk Are Key Enzymes Regulating the Growth of Mycoplasma Bovis DOI Creative Commons
Fei Yang, Mengmeng Yang, Fan Liu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 2012 - 2012

Published: Oct. 3, 2024

is a global problem for the cattle industry due to its high infection rates and associated morbidity, although pathophysiology poorly understood. In this study,

Language: Английский

Citations

1
Ultra-High-Performance Liquid Chromatography–Orbitrap-MS-Based Untargeted Lipidomics Reveal Lipid Characteristics of a Clinical Strain of Mycoplasma bovis from Holstein Cow DOI Creative Commons
Fei Yang, Mengmeng Yang, Fan Liu

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 11(11), P. 577 - 577

Published: Nov. 18, 2024

is a global concern for the cattle industry owing to its high rates of infection and resulting morbidity, but there are no reports on lipid composition metabolic pathways. This study examined lipidomics

Language: Английский

Citations

0
Macrophage extracellular traps are induced by Mycoplasma bovis in bovine macrophages through NADPH oxidase/ROS‐dependent manner and their antibacterial efficacy DOI Creative Commons
Ahmed Adel Baz, Shengli Chen,

Huafang Hao

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 9, 2024

Mycoplasma bovis has emerged as a significant pathogen in cattle, leading to considerable economic losses the cattle industry. It is associated with various clinical syndromes, including pneumoniae, mastitis, and arthritis. The innate immune response, particularly macrophages, plays crucial role combating infections caused by such pathogens. release of macrophage extracellular traps (METs) represents novel defense mechanism employed macrophages; however, impact M. on formation METs bovine macrophages remains unknown. Therefore, primary objective this study investigate which affects evaluate their bactericidal efficacy. Our findings revealed that cell lines released DNA fibrils colocalized histones cellular proteins response infection, visualized confocal scanning electron microscopy. Moreover, was found depend NADPH oxidase, for reactive oxygen species generation. Importantly, led lysis indicated lactate dehydrogenase, process be independent apoptosis pyroptosis. infection effective both killing restricting its growth. Overall, our first described how were produced responded demonstrated function bacterial killing, helpful improve understanding host's against pathogen.

Language: Английский

Citations

0