Public Health Threats Posed by Biofilms and Innovative Strategies for their Control DOI Open Access

Syed Hamza Abbas,

Shahzar Khan,

M.J. Shah

et al.

Discoveries, Journal Year: 2024, Volume and Issue: 12(4), P. e197 - e197

Published: Dec. 31, 2024

Biofilms are communities of microorganisms that adhere to surfaces within a self-produced protective matrix. The structural complexity biofilms and their inherent resistance conventional antimicrobial treatments make them significant public health challenge. These microbial communities, embedded extracellular matrix, associated with numerous persistent infections, especially those occurring in healthcare settings where they colonize medical devices chronic wounds. effects go beyond environments persist water treatment facilities, food processing plants, nature, which aid pollution transmission disease. This review article discusses multifaceted complications related the search for existing control strategies, process biofilm formation, mechanisms persistence, limitations traditional approaches. Additionally, this explores new innovative solutions, such as bacteriophage therapy, matrix-degrading enzymes, quorum sensing inhibitors. potential combination agents biofilm-disrupting compounds improvement efficacy is also paid special attention. seeks contribute these ongoing efforts by presenting an overview biology assessing variety possible strategies. Subsequently, insights derived from study may be used inform future research directions development more effective interventions biofilm-associated infections contamination various settings.

Language: Английский

Mycoplasma bovis : A review of vaccination and diagnostic initiatives DOI

Isaac Dayo Olorunshola,

Kabiru Ahmad, A.R. Peters

et al.

CABI Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Mycoplasma bovis infections in cattle constitute a worldwide problem with significant detrimental economic impacts on industry. Mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disorders are its clinical manifestations. Presently, no vaccines commercially available; antimicrobial resistance is increasing; diagnostic sensitivity testing needs to be improved; new rapid diagnosis kits eminent for implementation of treatment antimicrobials. We conducted systematic search databases such as PubMed, Scopus, Web Science, Google Scholar, AGRIS African Journals Online (AJOL), from 1995 2024. Searched keywords, as, bovis, M : vaccine development, techniques strain variability using the predefined criteria were used address review objectives. Although they have preventative function, – killed, live attenuated, subunit types face difficulties because M. strains vary widely. Every approach has own set benefits drawbacks, those that been studies include conventional culture identification, serological testing, immunohistochemical demonstration tissues, sophisticated molecular like PCR, qPCR, next-generation sequencing. For early detection, successful treatment, vaccination efficacy monitoring, accurate crucial. Future directions managing -associated diseases improving accessibility creating broad-spectrum vaccinations. By incorporating these developments, it may possible enhance health cattle, promote sustainability livestock production, increase food security. This points urgent need further research innovation advancement support

Language: Английский

Citations

0
Metagenomic analysis reveals the diversity, transmission and potential ecological risks of yak nasal bacteria-carried antibiotic resistance genes in the Sichuan region of Qinghai-Tibet plateau DOI Creative Commons

Zhao Fang-fang,

Shifeng Wu,

Xiuying Shen

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 494, P. 138476 - 138476

Published: May 8, 2025

Language: Английский

Citations

0
Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms DOI Creative Commons
Bryan Tegner Jacobson,

Jessica DeWit-Dibbert,

Eli T. Selong

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2650 - 2650

Published: Dec. 20, 2024

Mycoplasma spp. are facultative pathogens that contribute to the pathogenesis of multiple bovine diseases, including respiratory disease complex, and have been shown form biofilms. Biofilm formation is associated with increased antibiotic resistance in many organisms, but accurate determination antimicrobial susceptibility biofilms challenging. In spp., routinely determined using metabolic pH-dependent color change. However, biofilm can lead reduced metabolism, making interpretation readouts difficult. Therefore, we developed optimized a new flow cytometry-based method for testing biofilm-forming Mycoplasma, termed live/dead test (LD-AST). The LD-AST measures proportion live bacteria upon exposure antibiotics, works robustly both planktonic cultures, enables minimum bactericidal concentration (MBC) given antibiotic. We used two strains bovis (Donetta PG45 Madison) clinical bovoculi isolates (MVDL1 MVDL2) determine impact growth on gentamicin, enrofloxacin, or tetracycline. All were susceptible all antibiotics when cultured as cells, MBCs expected range. three out four PG45, MVDL1, completely resistant newly adhered analyzed, whereas M. Madison gave variable results. For mature 4-5 days before exposure, results also variable, some showing an certain decreased others. Overall, these consistent earlier reports exhibit resistance.

Language: Английский

Citations

0
Public Health Threats Posed by Biofilms and Innovative Strategies for their Control DOI Open Access

Syed Hamza Abbas,

Shahzar Khan,

M.J. Shah

et al.

Discoveries, Journal Year: 2024, Volume and Issue: 12(4), P. e197 - e197

Published: Dec. 31, 2024

Biofilms are communities of microorganisms that adhere to surfaces within a self-produced protective matrix. The structural complexity biofilms and their inherent resistance conventional antimicrobial treatments make them significant public health challenge. These microbial communities, embedded extracellular matrix, associated with numerous persistent infections, especially those occurring in healthcare settings where they colonize medical devices chronic wounds. effects go beyond environments persist water treatment facilities, food processing plants, nature, which aid pollution transmission disease. This review article discusses multifaceted complications related the search for existing control strategies, process biofilm formation, mechanisms persistence, limitations traditional approaches. Additionally, this explores new innovative solutions, such as bacteriophage therapy, matrix-degrading enzymes, quorum sensing inhibitors. potential combination agents biofilm-disrupting compounds improvement efficacy is also paid special attention. seeks contribute these ongoing efforts by presenting an overview biology assessing variety possible strategies. Subsequently, insights derived from study may be used inform future research directions development more effective interventions biofilm-associated infections contamination various settings.

Language: Английский

Citations

0