Microcystin-LR Induces Lipid Metabolism Disorder in Pelophylax nigromaculatus Tadpoles via the Gut–Liver Axis

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Disruption of lipid homeostasis in aquatic animals poses serious health risks, including tissue damage and systemic metabolic dysfunction. The precise mechanisms by which microcystin-LR, a potent cyanotoxin, disrupts metabolism amphibian tadpoles remain unclear. In this study, (Pelophylax nigromaculatus) were exposed to MC-LR fecal microbiota transplantation (FMT) experiments performed investigate whether or how at environmental concentrations interfered with tadpole from the perspective gut microbiota-gut-liver axis. Following exposure, liver exhibited significant inflammation, hypertrophy, fibrosis, accompanied elevated serum levels. Furthermore, expression levels farnesoid X receptor (FXR), nuclear receptor, significantly downregulated. Molecular docking molecular dynamics simulations indicated strong stable binding between FXR MC-LR. Moreover, suppressed activity, triggering: (1) upregulation sterol regulatory element-binding protein 1 (SREBP1)-mediated triglyceride (TG) synthesis, (2) inhibition free fatty acid (FFA) β-oxidation, (3) activation SREBP2-dependent bile biosynthesis. altered composition specific (e.g., taurocholic glycochenodeoxycholic acid) gut, thereby interfering hepatic metabolism, as evidenced FMT-induced accumulation recipient tadpoles. These findings identify potentially key target for suggest that changes intestinal also may be an important pathway driving dysregulation amphibians

Language: Английский

Growth performance, antioxidant status, intestinal morphology, and body composition of Nile tilapia (Oreochromis niloticus) supplemented with essential oils: A meta-analysis

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 176, P. 105353 - 105353

Published: July 4, 2024

Language: Английский