Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes DOI Creative Commons
Sara Gomes-Gonçalves,

Maria João Feiteiro,

Guilherme Moreira

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 325 - 325

Published: April 2, 2025

Research on endoparasites in pet dogs has been growing, but shepherd have largely overlooked. These frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes Blastocystis, also their owners. This contact increases the potential for to act as intermediates transmission Blastocystis. To clarify role these this parasite, study investigated presence Blastocystis dogs. Stool samples from Portuguese were analyzed using SYBR-Green-based real-time PCR and melting curve analysis followed by targeted-amplicon NGS mixed infections detection. Our results revealed a 60% occurrence sp. dog stools frequent identification ST1–ST4 ST14. Additionally, we observed subtype diversity within individual dogs, suggesting cross-species between livestock humans.

Language: Английский

Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes DOI Creative Commons
Sara Gomes-Gonçalves,

Maria João Feiteiro,

Guilherme Moreira

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 325 - 325

Published: April 2, 2025

Research on endoparasites in pet dogs has been growing, but shepherd have largely overlooked. These frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes Blastocystis, also their owners. This contact increases the potential for to act as intermediates transmission Blastocystis. To clarify role these this parasite, study investigated presence Blastocystis dogs. Stool samples from Portuguese were analyzed using SYBR-Green-based real-time PCR and melting curve analysis followed by targeted-amplicon NGS mixed infections detection. Our results revealed a 60% occurrence sp. dog stools frequent identification ST1–ST4 ST14. Additionally, we observed subtype diversity within individual dogs, suggesting cross-species between livestock humans.

Language: Английский

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