CD40 Ligand Potentiates Immunogenecity of Mycoplasma pneumoniae Subunit Vaccine Candidate in a Murine Model DOI Creative Commons
Jinqi Shu, Gaojian Li, Jianhong Shu

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 37 - 37

Published: Jan. 9, 2025

Mycoplasma hyopneumoniae (Mhp) infection severely affects the daily weight gain and feed-to-meat ratio of pigs, while secondary infections with other pathogens can further lead to increased mortality, causing significant economic losses pig industry. CD40L is a molecular adjuvant that enhances cellular humoral immune responses vaccines. In this study, peptide was fused C-terminus chimeric P97R1P46P42 protein by genetic engineering using pFastBac Dual vector. The recombinant its fusion P97R1P46P42-CD40L were expressed in Sf9 cells purified. Mice immunized or protein. Seppic ISA 201 emulsified protein, conventional Mhp vaccine PBS control groups included. Immunogenecity assessed specific IgG antibody response, splenic lymphocyte proliferation, cytokine IL-4 IFN-γ levels. We found significantly enhanced proliferation level mouse sera as compared group. This study provides clear evidence potentiates model. CD40L-fused could be MPS subunit candidate tested for efficacy pigs response challenges pathogenic strain(s).

Language: Английский

CD40 Ligand Potentiates Immunogenecity of Mycoplasma pneumoniae Subunit Vaccine Candidate in a Murine Model DOI Creative Commons
Jinqi Shu, Gaojian Li, Jianhong Shu

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(1), P. 37 - 37

Published: Jan. 9, 2025

Mycoplasma hyopneumoniae (Mhp) infection severely affects the daily weight gain and feed-to-meat ratio of pigs, while secondary infections with other pathogens can further lead to increased mortality, causing significant economic losses pig industry. CD40L is a molecular adjuvant that enhances cellular humoral immune responses vaccines. In this study, peptide was fused C-terminus chimeric P97R1P46P42 protein by genetic engineering using pFastBac Dual vector. The recombinant its fusion P97R1P46P42-CD40L were expressed in Sf9 cells purified. Mice immunized or protein. Seppic ISA 201 emulsified protein, conventional Mhp vaccine PBS control groups included. Immunogenecity assessed specific IgG antibody response, splenic lymphocyte proliferation, cytokine IL-4 IFN-γ levels. We found significantly enhanced proliferation level mouse sera as compared group. This study provides clear evidence potentiates model. CD40L-fused could be MPS subunit candidate tested for efficacy pigs response challenges pathogenic strain(s).

Language: Английский

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