Fine Mapping and Identification of a Fusarium Wilt Resistance Gene FwS1 in Pea DOI Creative Commons

Dong Deng,

Suli Sun, Wenqi Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Abstract Pea Fusarium wilt, incited by Fusarium oxysporum f. sp. pisi (Fop), has always been a devastating disease that causes severe yield losses and economic damage in pea-growing regions worldwide. The utilization of pea cultivars carrying resistance gene is the most efficient approach for managing this disease. In order to finely map gene, F2 population was established through cross between Shijiadacaiwan 1 (resistant) Y4 (susceptible). genetic analysis indicated Fop governed single dominant named as FwS1. Based on bulked segregant sequencing (BSA-seq) analyses, FwS1 initially detected chromosome 6 (i.e., linking group Ⅱ, chr6LG2), subsequent linkage mapping with 589 individuals fine-mapped FwS1 into 91.4 kb region. further functional annotation haplotype confirmed Psat6g003960, characterized NB-ARC (nucleotide-binding adaptor shared APAF-1, R proteins, CED-4) domain, considered promising candidate gene. encoding amino acids were altered “T/C” single-nucleotide polymorphism (SNP) first exon resulting observed differences peas. SNP locus, molecular marker A016180 determined be diagnostic validating its specificity both accessions populations different backgrounds. with KASP could facilitate marker-assisted selection breeding pea. addition, upon comparing gene Psat6g003960 74SN3B SJ1, it noted their sequences are identical, suggesting Fwf may same against wilt.

Language: Английский

Antifungal Activity of Selenium Nanoparticles Obtained by Plant-Mediated Synthesis DOI Creative Commons
Hugo Gerardo Lazcano-Ramírez,

Jorge Jonathan Oswaldo Garza‐García,

José A. Hernández-Díaz

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(1), P. 115 - 115

Published: Jan. 8, 2023

The continuous need to satisfy world food demand has led the search for new alternatives combat economic losses in agriculture caused by phytopathogenic fungi. These organisms cause plant diseases, reducing their productivity and decreasing fruit quality. Among tools being explored is nanotechnology. Nanoparticles with antimicrobial properties could be an excellent alternative address this problem. In work, selenium nanoparticles (SeNPs) were obtained using extracts of Amphipterygium glaucum leaves (SeNPs-AGL) Calendula officinalis flowers (SeNPs-COF). Characterization SeNPs was performed ability as antifungal agents against two commercially relevant pathogenic fungi, Fusarium oxysporum Colletotrichum gloeosporioides, evaluated. Assays different concentrations (0, 0.25, 0.5, 1.0, 1.7 mg/mL). It observed that both had activity pathogens at 0.25 mg/mL above. SeNPs-AGL demonstrated better smaller size (around 8.0 nm) than SeNPs-COF (134.0 nm). FTIR analysis evidenced existence functional groups constitute types SeNPs. There are factors have considered such nanoparticle phytochemical composition used, these may affect bioavailability.

Language: Английский

Citations

46
Breeding and Genomic Approaches towards Development of Fusarium Wilt Resistance in Chickpea DOI Creative Commons
Rakesh Kumar Yadav, M. K. Tripathi, Sushma Tiwari

et al.

Life, Journal Year: 2023, Volume and Issue: 13(4), P. 988 - 988

Published: April 11, 2023

Chickpea is an important leguminous crop with potential to provide dietary proteins both humans and animals. It also ameliorates soil nitrogen through biological fixation. The affected by array of biotic abiotic factors. Among different stresses, a major fungal disease called Fusarium wilt, caused

Language: Английский

Citations

43
Fusarium wilt of banana: Current update and sustainable disease control using classical and essential oils approaches DOI Creative Commons

Abubakar Abubakar Ismaila,

Khairulmazmi Ahmad,

Y. Siddique

et al.

Horticultural Plant Journal, Journal Year: 2022, Volume and Issue: 9(1), P. 1 - 28

Published: March 4, 2022

Fusarium species were reported to produce biofilms. Biofilms are superficial societies of microbes bounded and endangered by being situated or taking place outside a cell cells. The most destructive fungal diseases caused phytopathogens as result biofilms formation. wilt banana (Panama disease) is soil-borne pathogen called oxysporum f. sp. cubense. occurs in form complex (FOSC) which encompasses crowd strains. Horizontal genetic factor transfer may donate the observed assortment pathogenic strains, while sexual reproduction unknown FOSC. notorious disease on several crops worldwide. Yield loss this huge, significant destroy crop yields annually, thereby affecting producer countries various continents world. also resistant synthetic chemical fungicides. However, excessive use fungicides during control could be lethal humans, animals, plants. This calls for alternative eco-friendly management targeting formation finally suppressing devastating phytopathogen. In review, we, therefore, described damage disease, concept filamentous biofilms, classical strategies, sustainable strategies using essential oils, prevention vegetables diseases.

Language: Английский

Citations

45
Recent advancement in OMICS approaches to enhance abiotic stress tolerance in legumes DOI Creative Commons
Amjad Ali, Muhammad Tanveer Altaf, Muhammad Azhar Nadeem

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 28, 2022

The world is facing rapid climate change and a fast-growing global population. It believed that the population will be 9.7 billion in 2050. However, recent agriculture production not enough to feed current of 7.9 people, which causing huge hunger problem. Therefore, feeding 2050 target. Climate becoming threat agricultural production, it expected become worst upcoming years. Keeping this view, very important breed climate-resilient plants. Legumes are considered an pillar system great source high-quality protein, minerals, vitamins. During last two decades, advancements OMICs technology revolutionized plant breeding emerged as crop-saving tool wake change. Various approaches like Next-Generation sequencing (NGS), Transcriptomics, Proteomics, Metabolomics have been used legumes under abiotic stresses. scientific community successfully utilized these platforms investigated Quantitative Trait Loci (QTL), linked markers through genome-wide association studies, developed KASP can helpful for marker-assisted legumes. Gene-editing techniques proven soybean, cowpea, chickpea, model such Medicago truncatula Lotus japonicus . A number efforts made perform gene editing Moreover, did job identifying various genes involved metabolic pathways utilizing resulted information development legume cultivars at pace. review highlights contribution stresses We envisage presented develop cultivars.

Language: Английский

Citations

40
Approaches to Enhance Abiotic and Biotic Stress Tolerance in Leguminous Crops and Microgreens DOI
Priya Sharma, Anita Kumari

Published: Jan. 1, 2025

Language: Английский

Citations

1
Hollow Mesoporous Silica Nanoparticles as a New Nanoscale Resistance Inducer for Fusarium Wilt Control: Size Effects and Mechanism of Action DOI Open Access

Chaopu Ding,

Yunfei Zhang,

Chongbin Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4514 - 4514

Published: April 20, 2024

In agriculture, soil-borne fungal pathogens, especially Fusarium oxysporum strains, are posing a serious threat to efforts achieve global food security. the search for safer agrochemicals, silica nanoparticles (SiO2NPs) have recently been proposed as new tool alleviate pathogen damage including wilt. Hollow mesoporous (HMSNs), unique class of SiO2NPs, widely accepted desirable carriers pesticides. However, their roles in enhancing disease resistance plants and specific mechanism remain unknown. this study, three sizes HMSNs (19, 96, 406 nm HMSNs-19, HMSNs-96, HMSNs-406, respectively) were synthesized characterized determine effects on seed germination, seedling growth, f. sp. phaseoli (FOP) suppression. The exhibited no side cowpea germination growth at concentrations ranging from 100 1500 mg/L. inhibitory FOP mycelial very weak, showing inhibition ratios less than 20% even 2000 Foliar application HMSNs, however, was demonstrated reduce severity roots size- concentration-dependent manner. low concentration mg/L, well HMSNs-19 high 1000 observed little effect alleviating incidence. HMSNs-406 most effective an up 40.00% decline with significant growth-promoting plants. Moreover, foliar (1000 mg/L) increased salicylic acid (SA) content by 4.3-fold, expression levels SA marker genes PR-1 (by 1.97-fold) PR-5 9.38-fold), its receptor gene NPR-1 1.62-fold), compared infected control Meanwhile, another resistance-related PAL also upregulated 8.54-fold. Three defense-responsive enzymes POD, PAL, PPO involved HMSNs-enhanced roots, varying degrees reduction activity. These results provide substantial evidence that exert wilt suppression activating SA-dependent SAR (systemic acquired resistance) responses rather directly suppressing growth. Overall, first time, our indicate role potent inducer serve low-cost, highly efficient, safe sustainable alternative plant protection.

Language: Английский

Citations

6
Leguminous Seedborne Pathogens: Seed Health and Sustainable Crop Management DOI Creative Commons
Eliana Dell’Olmo, Antonio Tiberini, Loredana Sigillo

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(10), P. 2040 - 2040

Published: May 19, 2023

Pulses have gained popularity over the past few decades due to their use as a source of protein in food and favorable impact on soil fertility. Despite being essential modern agriculture, these species face number challenges, such agronomic crop management threats from plant seed pathogens. This review’s goal is gather information distribution, symptomatology, biology, host range seedborne Important diagnostic techniques are also discussed part successful process health certification. Additionally, strategies for sustainable control provided. Altogether, data collected suggested basic criteria set up conscious laboratory approach.

Language: Английский

Citations

15
Nematicidal and insecticidal activity of ethyl acetate extracts from culture filtrates of Arabidopsis thaliana fungal endophytes DOI Creative Commons
Sandra Díaz-González, María Fe Andrés, Carlos González‐Sanz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Endophytic fungi, which live inside plant tissues without causing symptoms of disease, produce a variety bioactive secondary metabolites. These metabolites have applications in biopesticide development, providing eco-friendly alternatives to conventional protection products (PPPs). This study explores the nematicidal and insecticidal properties ethyl acetate (EtOAc) extracts from endophytic fungi isolated wild populations Arabidopsis thaliana central Spain. The effects these against plant-parasitic nematode Meloidogyne javanica , as well two common pest insects ( Myzus persicae Spodoptera littoralis ) were studied. Our results showed that 9 out 13 exhibited significant and/or activity, indicating their potential. Chemical analysis through GC-MS LC-MS revealed active contained great mostly extract-exclusive metabolites, rich chemical diversity was independent activity extract or origin fungal isolate. work highlights importance exploration into produced by endophytes, particularly those model plants like A. . research lays groundwork for future studies aimed at harnessing source products, contributing sustainable agricultural practices improved crop methods.

Language: Английский

Citations

0
Identification of in planta bioprotectants against Fusarium wilt in Medicago sativa L. (lucerne) from a collection of bacterial isolates derived from Medicago seeds DOI Creative Commons

Shenali Subodha Herath Dissanayakalage,

Jatinder Kaur, Saidi R. Achari

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Fusarium wilt caused by oxysporum f. sp. medicaginis (Fom) is an important disease affecting lucerne/alfalfa cultivations worldwide. Medicago sativa L. (lucerne) one of the major legume crops in global forage industry. This study aimed to identify bacteria capable biologically controlling pathogen through a comprehensive screening bacterial isolates obtained from domesticated and wild growing seeds. Using multi-tiered evaluation pipeline, including vitro, soil-free potting mix-based pathogenicity bioprotection assay systems, efficacy 34 derived seeds was initially evaluated against six strains vitro. (Fo) F5189, which has previously been characterized as isolate causing lucerne selected for planta assays. Lucerne cultivars Grazer Sequel, representing susceptible resistant genotypes were chosen assess progression. Pathogenicity time-course studies conducted understand temporal dynamics host-pathogen interactions bioprotectants. The symptoms scored using rating index developed this study. results indicated variability across isolates, with some suppressing both systems. Paenibacillus (Lu_MgY_007; NCBI: PQ756884) Pseudomonas (Lu_LA164_018; PQ756887) identified promising bioprotectants under tested growth conditions. highlighted critical role persistent biocontrol activity precise timing application achieving long-term suppression. Overall, observed reduction severity underscores potential these sustainable strategies managing cultivars. However, molecular-level analyses are warranted elucidate underlying mechanisms, offering valuable insights development more effective future agricultural

Language: Английский

Citations

0
Challenges and prospects for grain legume production in Uzbekistan: a review DOI
Dilfuza Egamberdieva, Farkhod Eshboev, Zafarjon Jabbarov

et al.

Environmental Sustainability, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Language: Английский

Citations

0