Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e30933 - e30933

Published: May 1, 2024

Global climate change-related water deficit negatively affect the growth, development and yield performance of multiple cereal crops, including durum wheat. Therefore, improvement water-deficit stress tolerance in wheat varieties arid semiarid areas has become imperative for food security. Herein, we evaluated deficiency resilience potential two marker-free transgenic lines (AlSAP-lines: K9.3 K21.3) under well-watered conditions at both physiological agronomic levels. These overexpressed AlSAP gene, isolated from halophyte grass Aeluropus littoralis, encoding a stress-associated zinc finger protein containing A20/AN1 domains. Under conditions, wild-type (WT) AlSAP-lines displayed comparable concerning all parameters. Ectopic transgene expression exerted no adverse effects on growth plants. significant differences plant height, leaf number, spike length, spikelet number were observed between WT However, compared to WT, exhibited greater dry matter production, flag area, improved net photosynthetic rate, stomatal conductance, use efficiency. Notably, 25% higher grain (GY) than plants conditions. The RT-qPCR-based selected stress-related gene (TdDREB1, TdLEA, TdAPX1, TdBlt101-2) analyses indicated genes enhancement AlSAP-durum potentially related resilience. Collectively, our findings support that ectopic enhances ability, thereby compensate GY loss semi-arid regions.

Language: Английский

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Phytohormonal homeostasis, chloroplast stability, and heat shock transcription pathways related to the adaptability of creeping bentgrass species to heat stress

PROTOPLASMA, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Language: Английский

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Characterization of TaMYB Transcription Factor Genes Revealed Possible Early-Stage Selection for Heat Tolerance in Wheat

International Journal of Plant Biology, Journal Year: 2025, Volume and Issue: 16(2), P. 41 - 41

Published: March 26, 2025

Wheat quality and quantity are challenged by increasing global temperature, which poses an urgent need for heat tolerance breeding in wheat. The identification of seedling-stage factors highly associated with reproductive-stage performance can enable early-stage selection enhance the efficiency effectiveness breeding. This study investigated myeloblastosis (MYB) gene family, one largest transcription factor (TF) families plants, its response to seedling- stress Genome-wide analysis MYB TF genes identified 876 TaMYB genes, 48 were selected qRT-PCR expression based on silico under abiotic stresses. Correlation quantitative real-time polymerase chain reaction (qRT-PCR) pattern a heat-tolerant genotype (Perenjori) two heat-sensitive genotypes (Brazil32 Yitpi) at seedling stage grain-filling five (TaMYB-327, TaMYB-049, TaMYB-030, TaMYB-226, TaMYB-023) four (TaMYB-232, TaMYB-343, TaMYB-305, TaMYB399) sensitivity As important stress-responsive these showed similar patterns between early late developmental stages, indicating existence correlation therefore providing theoretical basis

Language: Английский

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Combining Genetic and Phenotypic Analyses for Detecting Bread Wheat Genotypes of Drought Tolerance through Multivariate Analysis Techniques

Life, Journal Year: 2024, Volume and Issue: 14(2), P. 183 - 183

Published: Jan. 25, 2024

Successfully promoting drought tolerance in wheat genotypes will require several procedures, such as field experimentations, measuring relevant traits, using analysis tools of high precision and efficiency, taking a complementary approach that combines analyses phenotyping genotyping at once. The aim this study is to assess the genetic diversity 60 SSR (simple sequence repeat) markers collected from regions world select 13 them more genetically diverse be re-evaluated under conditions stress by estimating 30 agro-physio-biochemical traits. Genetic parameters multivariate were used compare genotype traits identify which are increasingly efficient detecting tolerance. Hierarchical cluster (HC) divided into five main categories tolerance: four tolerant (HT), eight (T), nine moderate (MT), six sensitive (S), 33 (HS). Six exhibit combination heritability (>60%) gain (>20%). Analyses principal components stepwise multiple linear regression together identified (grain yield, flag leaf area, stomatal conductance, plant height, relative turgidity, glycine betaine, polyphenol oxidase, chlorophyll content, grain-filling duration) screening tool effectively detects variation among used. HC three categories: T, MT, S, representing three, five, genotypes, respectively, completely identical discriminant analysis. But case markers, they classified both significantly correlated per Mantel test. associated with considered an assistance selection process for So, useful has successfully detected some drought-tolerant coupled our knowledge phenotypic genotypic basis genotypes.

Language: Английский

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Quantitative exploration and conservation of the medicinal plant resources used by the inhabitants of Northern Pakistan

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Language: Английский

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Salt–Alkali Tolerance Evaluation for Bermudagrass and Critical Indicator Screening at the Seedling Stage

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 825 - 825

Published: Aug. 4, 2024

The adaptability of bermudagrass genotypes to high-pH saline–alkali conditions was investigated through a comprehensive evaluation 38 during the seedling stage. For this purpose, two distinct treatments were established: exposure solution composed 45% NaCl, 5% Na2SO4, NaHCO3, and Na2CO3 (pH 10.0), distilled water as control. On 6th day treatment, eight physiological indicators measured. Compared with control, net photosynthetic rates, leaf content, chlorophyll content test decreased under stress. In contrast, soluble protein proline levels, malondialdehyde concentration, conductivity exhibited an increase. salt–alkali tolerance coefficients each indicator ranged from 0.24 8.54, variable coefficient 9.77% 62.82%. Based on coefficients, value (D) resistance (CSAC) for genotype calculated. Subsequently, classified into three clusters by hierarchical clustering analysis, Cluster I consisting 10 most tolerance, II intermediate tolerance. III comprised 18 showing lowest predictive model assessing in is = −0.238 + 0.106 × SACChlb 0.209 SACRWC 0.015 SACPro 0.284 SACProtein 0.051 SACPn. Notably, more vigorous showed lower damage saline stress compared III. Moreover, stepwise regression analysis pinpointed Chlb, RWC, Pro crucial evaluating genotypes.

Language: Английский

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Multivariate Analysis Techniques and Tolerance Indices for Detecting Bread Wheat Genotypes of Drought Tolerance

Diversity, Journal Year: 2024, Volume and Issue: 16(8), P. 489 - 489

Published: Aug. 10, 2024

Drought stress is one of the biggest hardships in wheat cultivation because strong negative relationship between water deficit and crop yields owing to a lower grain weight, shorter grain-filling period, slower rate, reduced quality. Genotype–environment interaction (GEN:ENV) generates selecting genotypes ideotypes due biased genetic estimates. Diverse strategies have been proposed respond urgent need for concurrent improvements yield performance stability. This study’s purpose was appraise variation GEN:ENV effects on components discover drought-stress-tolerant ideotypes. study evaluated 20 three consecutive seasons under non-stressful drought-stress conditions total six ENVs. The broad-sense heritability ranged from 0.54 0.82 based expected mean squares 0.60 0.90 plot mean, but other ways, it usually greater than 0.90. high values (σgen:env2) revealed effect that has expression traits. G01, G03, G06, G07, G08, G10, G12, G13, G16, G17, G18 were stable (GY), according additive main multiplicative biplot Based scores weighted average absolute (WAASB), G02, G04, G05, selected as high-performance GY, they all best genotype groups using WAASB-GY superiority index. From results obtained principal component analysis hierarchical clustering tolerance discrimination indices, G18, G19 are produce suitable conditions. In essence, combining approaches take into consideration stability can contribute significantly enhancing reliability recommendations novel genotypes.

Language: Английский

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Assessing Heat Stress Tolerance of Wheat Genotypes through Integrated Molecular and Physio-Biochemical Analyses

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 1999 - 1999

Published: Sept. 2, 2024

Heat as an abiotic stress significantly impairs the sustainable productivity of wheat (Triticum aestivum L.). To determine tolerance genotypes to heat stress, a comprehensive approach should be used that integrates simultaneous phenotyping and genotyping analyses. The aim this study is identify local heat-tolerant using simple sequence repeat (SSR) markers evaluate selected under field conditions for their stress. Of 12 SSR showed polymorphism, eight were associated with six important traits. use hierarchical cluster analysis (HC) based on led identification 13 varying results grouped into three distinct classes: tolerant (T), moderately (MT), sensitive (S). had significant effect 19 traits study, variation in between genotypes. exhibited range average thousand-kernel weight (TKW) values 40.56 44.85, while genotype (Yecora Rojo) TKW 35.45. Furthermore, two times higher levels antioxidants compared when exposed Among analyzed, favorable combination high heritability (>60%) genetic gain (>20%). Through integration principal component stepwise multiple linear regression, it was determined (grain yield, 1000-kernel weight, plant height, intercellular carbon dioxide, flag leaf area, grain filling duration) revealed differences HC resulted same division main categories observed markers. It worth noting Saudi wheat, including KSU106, KSU105, KSU115 genotypes, addition 16HTWYT-22 genotype, other tested, indicating its potential suitability agriculture Arabia. These contribute breeding programs focused developing varieties accelerate progress improvement programs.

Language: Английский

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Identification of Wheat Ideotype under Multiple Abiotic Stresses and Complex Environmental Interplays by Multivariate Analysis Techniques

Plants, Journal Year: 2023, Volume and Issue: 12(20), P. 3540 - 3540

Published: Oct. 11, 2023

Multiple abiotic stresses negatively impact wheat production all over the world. We need to increase productivity by 60% provide food security world population of 9.6 billion 2050; it is surely time develop stress-tolerant genotypes with a thorough comprehension genetic basis and plant’s capacity tolerate these complex environmental reactions. To approach goals, we used multivariate analysis techniques, additive main effects multiplicative interaction (AMMI) model for prediction, linear discriminant (LDA) enhance reliability classification, multi-trait genotype-ideotype distance index (MGIDI) detect ideotype, weighted average absolute scores (WAASB) recognize stability that are highly productive. Six tolerance multi-indices were test twenty grown under multiple stresses. The AMMI showed varying differences performance indices, which disagreed trait genotype used. G01, G12, G16, G02 selected as appropriate stable using MGIDI six multi-indices. biplot features (G01, G03, G11, G17, G18, G20) most had high across environments. pooled analyses (LDA, MGIDI, WAASB) G01 candidate. (G01) considered novel resource improving stabilizing programs Hence, if in an integrated manner, strongly support plant breeders multi-environment trials.

Language: Английский

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Physiological mechanisms regulating source-sink interactions and grain yield formation in heat-stressed wheat

Plant Stress, Journal Year: 2024, Volume and Issue: 14, P. 100654 - 100654

Published: Nov. 7, 2024

Language: Английский

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