Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques DOI Creative Commons
Rosa Armijos-González,

Pablo Ramón-Contento,

Augusta Cueva-Agila

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 21, 2023

Abstract Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, challenge regeneration in its natural environment makes it a vulnerable species. In this context, various treatments vitro conservation explants were evaluated present study. For short-term conservation, effects osmotic substances sorbitol, mannitol, sucrose at different concentrations assessed. Different MS B5 culture media also examined their impact on growth, budding, mortality, rooting over 12 months without subcultures. long-term by cryopreservation, two techniques tested: vitrification encapsulation-dehydration. Short-term preservation sorbitol resulted low minimal limited development new shoots compared to or although tissues could be recovered successfully from all storage conditions. After cryopreservation 45 days recovery, with lowest mortality (4%) control treatment, cultivated medium which proved useful cryoprotectant, followed encapsulation-dehydration treatment. conclusion, is possible conserve C. using techniques, while further assays are needed conservation.

Language: Английский

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm DOI Creative Commons
Elena Popova,

И.Е. Куличенко,

Haeng-Hoon Kim

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(4), P. 542 - 542

Published: April 2, 2023

Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened species with non-orthodox seeds or limited seed availability, as well cell root cultures useful biotechnology. With increasing success, arsenal cryopreservation methods has been developed applied to many material types. However, severe damage accumulating during multi-step procedure often causes reduced survival low regrowth, even when optimized protocol applied. The conditions at recovery stage play a vital role in supporting regrowth after and, optimized, may shift life-and-death balance toward positive outcome. In this contribution, we provide overview five main strategies available improve post-cryopreservation vitro materials their further proliferation development. particular, discuss modification medium composition (iron- ammonium-free), exogenous additives cope oxidative stress absorb toxic chemicals, modulation osmotic potential. Special attention paid growth regulators used various steps process induce desired morphological response cryopreserved tissues. Given studies on electron transport energy provision rewarmed materials, effects light-and-dark light quality. We hope that summary provides helpful guideline set references choosing have not cryopreserved. also propose step-wise be most sensitive cryopreservation-induced chemical stresses.

Language: Английский

Citations

29
In Vitro Regeneration, Micropropagation and Germplasm Conservation of Horticultural Plants DOI Creative Commons
Jean Carlos Bettoni, Min‐Rui Wang, Qiao‐Chun Wang

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(1), P. 45 - 45

Published: Jan. 2, 2024

In vitro tissue culture technologies provide novel tools for improving plant production [...]

Language: Английский

Citations

5
Development of efficient and sustainable droplet-vitrification cryoconservation protocol for shoot tips for long-term conservation of Dahlia germplasm DOI

R. Gowthami,

Subhash Chander, Ruchira Pandey

et al.

Scientia Horticulturae, Journal Year: 2023, Volume and Issue: 321, P. 112329 - 112329

Published: July 12, 2023

Language: Английский

Citations

11
Advances Towards Ex Situ Conservation of Critically Endangered Rhodomyrtus psidioides (Myrtaceae) DOI Creative Commons
Lyndle K. Hardstaff, Bryn Funnekotter, Karen D. Sommerville

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 699 - 699

Published: Feb. 24, 2025

Rhodomyrtus psidioides (G.Don) Benth. (Myrtaceae) is a critically endangered rainforest species from the east coast of Australia, where populations have severely and rapidly declined due to effects repeated myrtle rust infection. With very limited material available in wild freezing-sensitive seeds that prevented storage seed bank, ex situ conservation this exceptional has proven difficult. Material orchard grown at Australian Botanic Garden Mount Annan was successfully used initiate three new accessions into tissue culture cuttings, undertake cryopreservation experiments using droplet-vitrification (DV) protocol for both cultured shoot tips. Use seedling initiation effective, with 94–100% success rate semi-hardwood explants 50–62% softwood explants. Although no survival after observed, R. showed some tolerance desiccation exposure cryoprotective agents. Regeneration DV demonstrated only one tip precultured on basal medium containing 0.4 M sucrose incubated PVS2 20 min prior immersion liquid nitrogen. These results demonstrate value living collections botanic gardens research, highlight importance germplasm choice initiation, potential cryobiotechnologies plant species.

Language: Английский

Citations

0
Cryobanking: a valuable tool for conserving tropical plant genetic resources – insights from the National Gene Bank of Tropical Crops in China DOI
Min‐Rui Wang, Zhiying Li,

Yunliu Fu

et al.

Acta Horticulturae, Journal Year: 2025, Volume and Issue: 1421, P. 125 - 130

Published: Feb. 1, 2025

Language: Английский

Citations

0
Dynamics of amino acids in the shoot tips of Pogostemon yatabeanus during cryopreservation by droplet-vitrification method and its modifications DOI

Byeongchan Choi,

Hyoeun Lee, Elena Popova

et al.

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105244 - 105244

Published: April 14, 2025

Language: Английский

Citations

0
Cryopreservation research for long term conservation of Artocarpus lacucha Buch. -Ham. genetic resources DOI

Rinky Resma Panda,

Ramesh Chandra,

Sandhya Gupta

et al.

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Language: Английский

Citations

0
Scratching the surface: The in vitro research that will be critical for conserving exceptional plants to scale DOI Creative Commons
Valerie C. Pence, Emily Beckman Bruns

In Vitro Cellular & Developmental Biology - Plant, Journal Year: 2024, Volume and Issue: 60(3), P. 266 - 282

Published: April 26, 2024

Abstract The conservation of threatened exceptional plants, which cannot be conserved by seed banking, requires in vitro technologies for many the approaches needed their long-term ex situ conservation. This study evaluated current plant literature, as represented Web Science, to determine its taxonomic overlap with families and genera 775 species currently listed exceptional. Science was searched using terms micropropagation, somatic embryogenesis, zygotic embryo, cryopreservation, target were identified more than 19,000 articles evaluated. There five significant between literature species: Fabaceae, Asteraceae, Orchidaceae, Arecaceae, Rutaceae. However, there less at level genus, Citrus, Coffea, Quercus having most . Significant gaps also found, 14 half no representation search results. 20 all economically important species, these had 343 congeners that could prioritized research. A highly group plants significantly under-represented tropical woody form backbone diversity world’s rainforests. Overall, are areas strength upon build future work, but where research should effectively conserving plants.

Language: Английский

Citations

3
Yield prediction of root crops in field using remote sensing: A comprehensive review DOI

Hanhui Jiang,

Liguo Jiang,

Leilei He

et al.

Computers and Electronics in Agriculture, Journal Year: 2024, Volume and Issue: 227, P. 109600 - 109600

Published: Nov. 9, 2024

Language: Английский

Citations

3
Cryoconservation of Antiallergic Medicinal Plants: Techniques and Applications DOI

R. Gowthami,

Marcos Edel Martínez-Montero, P. E. Rajasekharan

et al.

Published: Jan. 1, 2024

Medicinal plants have long been integral to diverse traditional medicine systems, with many exhibiting remarkable antiallergic properties. However, several valuable herbs face threats from overexploitation, unregulated harvesting, unsustainable cultivation practices, and climate change impacts. This growing awareness of the need conserve these has led increased focus on ex situ conservation strategies, including field genebanks, botanical gardens, seed in vitro cryogenebanks. While genebanks gardens expose species potential risks like pest infestations natural disasters, is effective for certain species. exceptional plant characterized by limited production, challenging collection, seeds that cannot withstand conventional drying storage cryogenebank emerges as most viable option. Various cryopreservation techniques, encompassing preservation whole seeds, zygotic embryos, embryonic axes, dormant buds, offer store theoretically indefinitely chapter provides a comprehensive overview research methods other explants medicinal Additionally, successful case studies are presented.

Language: Английский

Citations

2