Optimizing Fermentation Strategies for Enhanced Tryptophan Production in Escherichia coli: Integrating Genetic and Environmental Controls for Industrial Applications DOI Open Access
Miguel Angel Ramos-Valdovinos, Agustino Martı́nez-Antonio

Processes, Journal Year: 2024, Volume and Issue: 12(11), P. 2422 - 2422

Published: Nov. 2, 2024

Tryptophan is an essential aromatic amino acid widely used in the pharmaceutical, agricultural, and feed industries. Microbial fermentation, mainly using Escherichia coli, has become preferred method for its production due to sustainability lower costs. Optimizing tryptophan requires careful control of various fermentation parameters, including nutrients, pH, temperature, dissolved oxygen (DO) levels. Glucose, as primary carbon source, must be fed at controlled rates avoid metabolic overflow, which leads by-product accumulation reduced efficiency. Nitrogen sources, both organic (such yeast extract) inorganic (like ammonium), influence biomass growth yield, with ammonium levels requiring regulation toxic accumulation. Phosphate enhances but can lead formation if excessively. pH another critical factor, optimal range between 6.5 7.2, where enzyme activity maximized. Temperature promotes production, particularly 30 °C 37 °C. High DO increase titers by boosting pentose phosphate pathway reducing by-products like acetate. Furthermore, surfactants supplements such betaine monohydrate citrate help alleviate osmotic stress enhance precursor availability, improving Careful manipulation these parameters allows high-density cell cultures significant accumulation, making microbial competitive large-scale production.

Language: Английский

The Nutritional Profile of Root Vegetables Through Spontaneous Fermentation with Apples: Amino Acid Composition and Microbial Dynamics DOI Creative Commons
Iwona Gientka,

Alicja Synowiec,

Katarzyna Pobiega

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 110 - 110

Published: Feb. 25, 2025

To diversify fermented plant-based products, vegetables can be with fruits. This study aimed to evaluate the effect of spontaneous fermentation on amino acid nutritional profile sliced carrot (Daucus carota) and celeriac (Apium graveolens), without addition apple (Malus domestica). Lactic bacteria rapidly lowered pH, ensuring product’s microbiological stability safety. The apples root had a beneficial maintaining high number active lactic bacteria. process significantly influenced composition, reducing content acidic acids (aspartic glutamic acid) while increasing levels several essential acids. Leucine was found limiting in all variants, although its increased during fermentation. Essential Amino Acid Index (EAAI) products generally higher variants (carrot = 1.13, 1.03). results indicate that enhances protein quality contributes development nutritionally valuable, microbiologically safe foods. supports sustainable dietary practices by utilizing locally available create innovative products.

Language: Английский

Citations

0

Vorax biofertilizer on off-season maize DOI Creative Commons
Nataniel Osmar Risse,

Daniel Petrich Espindola,

F M P Costa

et al.

Caderno Pedagógico, Journal Year: 2025, Volume and Issue: 22(5), P. e14990 - e14990

Published: March 24, 2025

The search for more sustainable agriculture has stimulated interest in organic fertilizers. rising costs of chemical fertilizers and the many benefits identified by use biofertilizers have motivated this trend. However, there are options on market assessing impact each yield crop development is therefore a key aspect making best choice. Vorax® Biostimulant composed glutamic acid, source carbohydrate absorbed plant. Therefore, aim work was to evaluate biostimulant, extracted from Corynebacterium glutamicum, corn off-season. Two experiments were carried out using biofertilizer different combinations dose, number applications phenological stages randomized block design with four replications. It found that when applied at V8 lower doses, maximum two applications, (non-significant) gain ear weight.

Language: Английский

Citations

0

Assessing the Role of Biostimulants in Affecting the Production of Radish and Eggplant DOI
Muhammad Amjad Bashir,

Qurat-Ul-Ain Raza,

Abdur Rehim

et al.

Journal of soil science and plant nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Language: Английский

Citations

0

Effects of exogenous amino acids on yttrium uptake and accumulation in tomato ( Solanum lycopersicum ) DOI
Ali Sajid,

Sidra Naseer,

Ming-Xun Ren

et al.

International Journal of Phytoremediation, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 9

Published: March 4, 2025

This study investigated the impacts of exogenous amino acid supplementation on uptake, translocation, and accumulation yttrium (Y) in tomato plants (Solanum lycopersicum). To understand how acids enhance nutrient uptake plant growth by using Hoagland solution. The results indicated that combination Y with glutamic (Y + Glu) significantly increased concentration leaves to 28.5 ± 1.42 µg g-1, while histidine His) resulted a markedly lower 2.7 0.06 g-1. Notably, proved be particularly effective enhancing xylem sap. control exhibited higher sap flow rate 0.27 0.008 g h-1, which was greater than those treated (p < 0.05). Histidine levels were elevated His treatment, reaching 194.78 13.79 μmol L-1, tryptophan aspartic showed their highest concentrations respective treatments at 109.92 14.43 L-1 212.95 13.65 L-1. These findings demonstrated substantially enhanced phytoextraction plants, through application acid. Further exploration into molecular mechanisms governing complexation transport within phytoremediation is needed.

Language: Английский

Citations

0

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants DOI Creative Commons
Dorota Wieczorek, Dorota Gendaszewska, Katarzyna Miśkiewicz

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(5)

Published: Sept. 14, 2023

ABSTRACT The yeast strain Yarrowia lipolytica IPS 21 was tested for its ability to degrade potentially toxic chrome-tanned leather shavings (CTLS) in a liquid environment. Biological and chemical parameters were monitored during 48-h period of biotransformation the protein-rich waste. CTLS added at concentration 0.1–4% (wt/wt) modified YPG medium (15 g L −1 extract 5 NaCl). Biodegradation bioconversion performed one-step process. It found that higher degradation rate depended on activity proteases pH medium, but not initial inoculum ratio dehydrogenase. highest efficiency process obtained 4% day 2 (degradation 58–67%, biomass production 2.11–2.20 , protease 312 U mg protein, 9.20). Our results showed total chromium probably transported across cytoplasmic membrane Y. IPS21 (III) oxidized (VI). phytotoxicity selected amino acid supernatants [2.5% (vol/vol)] after had stimulating effect plants tested. root elongation 29–28% than reference samples. This result makes potential candidate safely converting wastes into valuable products without enzyme isolation, e.g., fertilizers. IMPORTANCE Enzyme technologies have greatest practical relevance environmental trends. Overcoming barrier high cost carbon substrates used is main challenge these methods. huge use extracellular species or acids various industries indicates need extension basic research waste as source this yeast. experiments demonstrated it possible single-step produce high-value supernatant having an isolated enzyme. In our study, we show 2.5% (vol/vol) from system provide information factors migration chromium.

Language: Английский

Citations

4

Effects of L-glutamic acid application on yield, grain quality, photosynthetic pigments, 2-acetyl-1-pyrroline, and antioxidant system of aromatic rice DOI
Haowen Luo,

Meiyang Duan,

Pipeng Xing

et al.

Field Crops Research, Journal Year: 2023, Volume and Issue: 303, P. 109134 - 109134

Published: Sept. 19, 2023

Language: Английский

Citations

4

Selenization and its impact on quantitative and qualitative parameters of carrot juice. DOI Creative Commons
Ivana Mezeyová, Ján Mezey, Miroslav Šlosár

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 103933 - 103933

Published: March 28, 2024

Carrot juice is a rich source of vitamins, carotenes, minerals, and other micronutrients with positive health effects. The ´Kamaran F1´, ´Baltimore ´Naval ´Niagara ´Flakkese 2 – Vita longa´ varieties were included in the study conducted during years 2021–2022. examined influence selenization on selenium level additionally also qualitative quantitative indicators carrot juice. For total 50 mg/L sodium selenate was applied. process making involved cleaning washing roots, crushing, pressing them, pasteurizing for 5 min at 85 °C, aseptic filling. In addition to content, parameters (1 - root average weight, roots yield, yield) (carotenoids, sugars, acids, soluble solids) monitored. Our findings revealed higher that followed this processing method. content Se fresh without fortification 14 μg/L, while fortified variant 186 μg/L (13-fold increase). pasteurized 11-fold increase found variant. This indicates form thermostable, meaning retains its composition, adding additional value. Based assessed application recommended dose did not significantly affect height yields, which an important economic criterion.

Language: Английский

Citations

1

Respuestas fisiológicas y productivas de plantas de remolacha tratadas con una solución de aminoácidos DOI Creative Commons
Kolima Peña Calzada, Alexander Calero Hurtado,

Victoria Peistrup

et al.

Temas Agrarios, Journal Year: 2024, Volume and Issue: 29(1), P. 113 - 125

Published: Sept. 3, 2024

El crecimiento exponencial de la población mundial hace necesario buscar alternativas para incrementar producción alimentos. Los aminoácidos se utilizan ampliamente mejorar productividad los cultivos, sin embargo, en el cultivo remolacha roja no está generalizado su uso. Por lo que objetivo investigación fue evaluar efecto aplicación foliar una solución índices fisiológicos y roja. Se diseñó un experimento bloques al azar con cinco tratamientos seis réplicas. Fueron evaluadas cuatro concentraciones VA1 (0,3 ml L-1), VA2(0,6 VA3(0,9 VA4(1,2 L-1) tratamiento control VA0 (0). evaluó biomasa seca las plantas, área foliar, tasas crecimiento, contenido clorofilas totales, temperatura hojas, relativo agua rendimiento agrícola. resultados mostraron menor cuando usó incremento significativo plantas todas sus variantes. Las clorofilas, así como también fueron beneficiadas por aminoácidos. La mayor eficiencia productiva obtuvo 0,6; 0,9 1,2 L-1 mejor respuesta dosis L-1. es alternativa

Citations

1

Sustainable Use of Organic Seaweed Fertilizer Improves the Metagenomic Function of Microbial Communities in the Soil of Rice Plants DOI Open Access
Eka Sunarwidhi Prasedya, Nanda Sofian Hadi Kurniawan, Fitriani Fitriani

et al.

Sustainability, Journal Year: 2023, Volume and Issue: 15(23), P. 16328 - 16328

Published: Nov. 27, 2023

The frequent use of chemical fertilizers in agricultural practices has developed into a serious environmental concern which urgently needs solution to restrain their systems. Hence, there is an urgent need investigate potential organic from various natural resources decrease the fertilizers. Seaweed among with sustainability value. Our previous work shown effectiveness seaweed fertilizer for increasing plant growth and soil beneficial microbiota. This study aims evaluate functional genes present rice plants treated fertilization. It involves amendments reduced concentrations three groups: CF (only fertilizer), CFSF1 (50% dose + 1 ton/ha), CFSF2 2 ton/ha). supplemented were taller faster mature compared CF. In addition, primary macronutrients nitrogen (N), phosphorus (P), potassium (K) also significantly higher SF. findings showed increased ammonia-oxidizing archaea Crenarchaeota abundance SF treatments. PICRUSt analyses indicated enriched proteins relation amino acid, nucleotide, protein, carbohydrate metabolism based on KEGG, BioCyc, PFAM databases. current outcomes enhanced our understanding regarding importance microbial community quality. Furthermore, supplementation improvement fertility, increases productivity.

Language: Английский

Citations

2

Comparative metabolite profiling in single and multiple bulb cultivars of shallot (Allium cepa L. Aggregatum group) DOI Creative Commons
Fransiska Renita Anon Basundari, Endang Sulistyaningsih, Rudi Hari Murti

et al.

Journal of the Saudi Society of Agricultural Sciences, Journal Year: 2024, Volume and Issue: 23(8), P. 521 - 532

Published: June 15, 2024

Tuk-Tuk and Sanren F1 are two shallot cultivars that exhibit divergent bulb multiplication traits. typically produces one bulb. In contrast, tends to produce multiple bulbs. This study used metabolomics during development harvesting. The objective of the was identify metabolites might affect shallot. initial samples both were collected bulbs developmental stage i.e, 11 weeks after sowing (WAS), while second sample gathered harvesting (16 WAS). Proton Nuclear Magnetic Resonance (1H NMR) analysis applied freeze-dried for analysis. Multivariate utilizing Principal Component Analysis (PCA) followed by Orthogonal Projections Discriminant (OPLS-DA) conducted using MetaboAnalyst 5.0. 1H NMR spectra revealed 28 putative metabolites. All data, including harvesting, not clearly separated PCA. However in stage, observed have higher histidine than Tuk-Tuk, indicating involvement kinase signaling system multiplication. Hence, known its role cytokinin which contributing cell division regulation may Four i.e., sucrose, arginine, acetic acid, myricetin F1, a cultivar single Valine, phenylalanine, formic α-linolenic acid considerably There also several with greater relative concentrations others. Though all those significantly differed amongst examined cultivars, it thought they had an impact on development. It would be great interest ascertain whether detected at levels potential function as chemical markers distinguishing capable producing

Language: Английский

Citations

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