Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 25, 2025

Reverse transcription-quantitative PCR (RT-qPCR) is a powerful tool for quantifying gene expression. However, reference genes (RGs) expression analysis in peach (Prunus persica) during interactions with Monilinia laxa, major fungal pathogen that causes brown rot, have not been established. In this study, we analysed 12 candidate RGs pathosystem by analysing samples from to 144 HAI. The stability of the was evaluated using ΔCq method and BestKeeper, NormFinder, geNorm algorithms. Our results identified AKT3, RNA pol II (RPII) SNARE (using geNorm), RPII, AKT3 TEF2 NormFinder), RPII BestKeeper) MUB6 method) as most stable mRNA normalisation across all tested samples. algorithm used determine optimal number suitable required proper under these experimental conditions, indicating three were sufficient normalisation. Analysis obtained different algorithms showed RGs. Furthermore, confirm validity genes, levels six interest, involved metabolic pathways, normalized inoculated uninoculated fruit. These findings provide set accurate RT-qPCR studies involving M. laxa interactions, facilitating deeper insights into molecular mechanisms underlying important plant–pathogen relationship.

Language: Английский

Efficacy of biopesticides and fungicides against brown rot on cherry ( Prunus avium ) and plum ( Prunus domestica )

Biocontrol Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 4, 2025

Cherry and plum are prone to brown rot diseases. Synthetic fungicides still the main management tool control but there is a need develop eco-friendly methods. Five commercial biopesticides including BioPK (Trichoderma viride, T. harzianum longibranchum), BoniProtect (Aureobasidium pullulans), Trianum (T. harzianum), PreStop (Gliocladium catenulatum) Serenade (Bacillus subtilis) along with Indar (fenbuconazole, synthetic fungicide) were tested against Monilinia laxa which products applied before after pathogen inoculation. completely inhibited development. partially M. when The efficacy of two indigenous strains (B. subtilis B91 A. pullulans Y126) evaluated under ambient conditions or 1°C; used as treatments. first treated specific products, then wounded inoculated laxa. both conditions. Under conditions, only reduced incidence disease. When stored at 1°C for 4 weeks, all biocontrol treatments However, fruits left an additional 1 week, disease did not differ between Post-harvest application was studied Rovral control. submerged into individual 6 weeks. disease, whilst not. In conclusion, Serenade, Y126 can inhibit infection. present study supports that these proeducts should be pre-harvest reduce extent fruit latent infection by laxa, effective post-harvest cold chain implemented delay/minimise

Language: Английский

Deciphering the Interaction between Coniella granati and Pomegranate Fruit Employing Transcriptomics

Life, Journal Year: 2024, Volume and Issue: 14(6), P. 752 - 752

Published: June 13, 2024

Pomegranate fruit dry rot is caused by Coniella granati, also referred as Pilidiella granati. In order to decipher the induced responses of mature pomegranates inoculated with pathogen, an RNA-seq analysis was employed. A high number differentially expressed genes (DEGs) were observed through a three-time series inoculation period. The transcriptional reprogramming time-dependent, whereas majority DEGs suppressed and expression patterns specific may facilitate pathogen colonization at 1 day after (dai). contrast, 2 dai mainly thereafter 3 dai, defense partially triggered in delay. Particularly, upregulated latest time point. Among them, involved cell wall modification degradation processes, recognition signaling transduction cascades, activation metabolite biosynthesis-related genes, well induction particular families factors, constitute crucial components recruiting strategy employed pomegranate upon C. granati challenge. Overall, our findings provide novel insights compatible interaction pomegranates—C. lay foundations for establishing integrated pest management (IPM) strategies involving advanced approaches, such gene editing or molecular breeding programs disease resistance, according European Union (EU) goals.

Language: Английский