Isolation of Bacteria from Agricultural Soils and Evaluation of Their Degradative Capacity for Organochlorine and Organophosphorus Pesticides
Agronomy,
Journal Year:
2025,
Volume and Issue:
15(4), P. 814 - 814
Published: March 26, 2025
In
this
work,
OP-
and
OC-degrading
bacteria
were
isolated
from
agricultural
soil
samples
taken
in
the
department
of
Bolivar,
Colombia.
The
objective
research
was
to
degrade
organochlorine
organophosphorus
pesticides
using
bacterial
colonies
native
soils.
Two
samples,
which
showed
a
higher
degree
adaptation
media
contaminated
with
pesticide
mixtures.
They
identified
by
biochemical
tests
BBL
Crystal
kits,
and,
subsequently,
their
16S
rDNA
sequenced
PCR
technique.
Bacterial
growth
studied
OD
index,
taking
absorbance
readings
on
UV-VIS
spectrophotometer
at
600
nm,
0.5
McFarland
scale,
quantification
degradation
GC–MS.
Bacillus
cereus
Paenibacillus
lautus.
B.
isolates
exposed
OPs
malathion,
chlorpyrifos,
coumaphos
[80
mg·L−1],
degrading
rates
52.4%,
78.8%
79.5%,
respectively,
after
12
days
incubation
liquid
medium
pH
=
7.0
±
0.2
37
°C.
Furthermore,
P.
lautus
OCs
lindane,
metolachlor,
endrin,
p,p′-DDT
mg·L−1]
degraded
64.0%,
60.8%,
55.7%
65.1%
under
same
conditions
temperature,
pH,
time.
These
results
show
that
might
be
useful
for
cleaning
up
environments
have
been
polluted
OCs.
Language: Английский
Integrated Approaches to Eco-Friendly Processes for Persistent Pollutants Contamination
Processes,
Journal Year:
2024,
Volume and Issue:
12(12), P. 2812 - 2812
Published: Dec. 9, 2024
All
life
on
Earth
is
fully
affected
by
the
environment’s
health
and
sustainability
[...]
Language: Английский