Combination of humic biostimulants with a microbial inoculum improves lettuce productivity, nutrient uptake, and primary and secondary metabolism

Plant and Soil, Journal Year: 2022, Volume and Issue: 481(1-2), P. 285 - 314

Published: Aug. 8, 2022

Abstract Background and aims Biostimulants of natural origin represent a growing ecological strategy to increase crops productivity, especially when applied in combination with microbial bioeffectors. We studied the effect biostimulants such as Potassium Humates (KH) from Leonardite Compost Tea (CT) green compost on both productivity nutritional status lettuce plants, well primary secondary metabolism treated amended either alone or commercial inoculum (M+), mainly based arbuscular mycorrhizal fungi (Micosat TabPlus). Results The biomass production uptake macro- micronutrients by plants significantly increased mixture humic materials (MIX) combined inoculum. Similarly, synergic MIX_M+ treatment affected more than their individual applications, increasing, respectively, biosynthesis essential amino acids carbohydrates, that antioxidant polyphenolic compounds, hydroxycinnamic acids, flavonols coumarins. Conclusions Our findings suggest calibrated bioactive molecules consortia represents potential tool improve crop its metabolic status.

Language: Английский

---

Designing a synthetic microbial community devoted to biological control: The case study of Fusarium wilt of banana

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 5, 2022

Fusarium oxysporum f. sp. cubense ( Foc ) tropical race 4 (TR4) is threatening banana production because of its increasing spread. Biological control approaches have been widely studied and constitute interesting complementary measures to integrated disease management strategies. They based mainly on the use single biological agents (BCAs). In this study, we moved a step forward by designing synthetic microbial community (SynCom) for wilt (FWB). Ninety-six isolates Pseudomonas spp., Bacillus Streptomyces Trichoderma spp. were obtained from rhizosphere selected in vitro antagonism against TR4. pot experiments, large such as SynCom 1.0 (44 with moderate high antagonistic activity) or small one 1.1 (seven highly effective isolates) provided similar (35% symptom severity reduction). An study interactions among between them revealed that beneficial microorganisms not only antagonized pathogen but also some constituents. Furthermore, defended itself antagonizing microbes. We demonstrated fusaric acid, known secondary metabolites species, might be involved an interaction. With knowledge, 1.2 was then designed three isolates: chlororaphis subsp. piscium PS5, velezensis BN8.2, virens T2C1.4. A non-simultaneous soil application these (to diminish cross-inhibition) delayed FWB progress over time, significant reductions incidence severity. performed better than two commercial BCAs, BioPak ® T-Gro. Eventually, characterized several biocontrol traits their genome sequenced. Our data showed assembling easy task. The mere mixtures antagonists (e.g., 1.1) provide biocontrol, accurate investigation needed improve results 1.2). valuable tool further developed FWB.

Language: Английский

---

Combating biotic stresses in plants by synthetic microbial communities: Principles, applications and challenges

Journal of Applied Microbiology, Journal Year: 2022, Volume and Issue: 133(5), P. 2742 - 2759

Published: Aug. 30, 2022

Abstract Presently, agriculture worldwide is facing the major challenge of feeding increasing population sustainably. The conventional practices have not only failed to meet projected needs, but also led tremendous environmental consequences. Hence, ensure a food-secure and environmentally sound future, thrust on sustainable alternatives. Due challenges associated with means application biocontrol agents in management biotic stresses agroecosystems, significant transformations this context are needed. crucial role played by soil microbiome efficiently sustainably managing agricultural production has unfolded newer approach rhizosphere engineering that shows immense promise mitigating an eco-friendly manner. strategy generating synthetic microbial communities (SynComs), integrating omics approaches traditional techniques enumeration in-depth analysis plant–microbe interactions, encouraging. review discusses significance rhizospheric plant's fitness, its manipulation for enhancing plant attributes. focus critically analyse potential tools design utilization SynComs as ameliorate plants. Furthermore, based synthesis reports area, we put forth possible solutions some critical issues impair large-scale agriculture.

Language: Английский

---

Microbial Community and Function-Based Synthetic Bioinoculants: A Perspective for Sustainable Agriculture

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: March 11, 2022

Interactions among the plant microbiome and its host are dynamic, both spatially temporally, leading to beneficial or pathogenic relationships in rhizosphere, phyllosphere, endosphere. These interactions range from cellular molecular genomic levels, exemplified by many complementing coevolutionary relationships. The plants acquire metabolic developmental traits such as alteration their exudation pattern, acquisition of systemic tolerance, coordination signaling metabolites interact with microbial partners including bacteria, fungi, archaea, protists, viruses. responds gaining losing various signals environment. Such adaptive make way for coexistence, living together on, around, inside plants. have been exploited using traditional culturable approaches isolating microbes target functions, clearly contributing toward plants’ growth, fitness, stress resilience. new knowledge gained on unculturable members metagenome research has indicated predominance particular phyla/genera presumptive functions. Practically, approach gives hand direct use, whereas perfect theoretical information about taxonomy potential well-colonized major groups associated To capitalize beneficial, endemic, functionally diverse microbiome, strategic concomitant use culture-dependent culture-independent techniques would help designing novel “biologicals” crops. designed biologicals (or bioinoculants) should ensure community’s persistence due functional abilities. Here, we discuss current paradigm plant-microbiome-induced functions strategies synthesizing bioinoculants based phylum communities approaches. effective crop-specific inclusive community may lead reduction cost cultivation improvement soil health sustainable agriculture.

Language: Английский

---

Biocontrol of plant pathogens in omics era—with special focus on endophytic bacilli

Critical Reviews in Biotechnology, Journal Year: 2023, Volume and Issue: 44(4), P. 562 - 580

Published: April 13, 2023

Nearly all plants and their organs are inhabited by endophytic microbes which play a crucial role in plant fitness stress resilience. Harnessing services can provide effective solutions for sustainable increase agriculture productivity be used as complement or alternative to agrochemicals. Shifting practices toward the use of nature-based contribute directly global challenges food security environmental sustainability. However, microbial inoculants have been several decades with inconsistent efficacy. Key reasons this efficacy linked competition indigenous soil microflora inability colonize plants. Endophytic both these issues potentially make them better candidates inoculants. This article outlines current advancements research special focus on bacilli. A understanding diverse mechanisms disease control bacilli is essential achieve maximum biocontrol against multiple phytopathogens. Furthermore, we argue that integration emerging technologies strong theoretical frameworks potential revolutionize approaches based microbes.

Language: Английский

---