Recent advancements in bacterial anti-phage strategies and the underlying mechanisms altering susceptibility to antibiotics

Microbiological Research, Journal Year: 2025, Volume and Issue: 295, P. 128107 - 128107

Published: Feb. 21, 2025

Language: Английский

Synergistic antibacterial activity of curcumin and phage against multidrug-resistant Acinetobacter baumannii

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 15, 2025

Acinetobacter baumannii is a priority bacterial pathogen and leading cause of nosocomial infections, particularly in intensive care units (ICUs). The average incidence carbapenem-resistant A. infections ICUs 41.7 cases/1,000 patients, highlighting the urgent need for more effective alternative therapies to replace carbapenems. Thus, this study aimed investigate first time antibacterial activity curcumin combination with novel phage vB_AbaSI_1 combat multidrug-resistant (MDR) vitro. Phage (capsid diameter 91 nm, contractile tail 94/20 nm) was isolated from sewage infects ~ 29% 131 isolates examined. 52,783 kb genome has 75 ORFs, encodes an integrase, lacks tRNAs/virulence genes, belongs Caudoviricetes. Commercially sourced (400 µg/mL), combined (MOI 100) reduced MDR undetectable levels 1 h post-treatment at 37 °C, efficacy further extended 5 double-dosed phage/curcumin-treated cultures. In contrast, treatment just growth but rebounded within 3 h, while curcumin-only treated cultures showed only 1-log reduction compared untreated control. phage/curcumin synergy occurred exclusively phage-susceptible strains pre-curcumin exposure. This suggests potential disruption cell membrane during infection allowing entry, as no observed phage-resistant strains. innovative strategy combining great controlling therapeutic deployment. Future work will focus on engineering make it therapeutically acceptable.

Language: Английский

Bacteriophage Resurrection: Innovative Impacts in Medicine, Biotechnology, and Environmental Solutions

Scientific African, Journal Year: 2024, Volume and Issue: unknown, P. e02506 - e02506

Published: Dec. 1, 2024

Language: Английский