Journal of Separation Science,
Journal Year:
2025,
Volume and Issue:
48(2)
Published: Feb. 1, 2025
ABSTRACT
The
aim
of
this
research
was
the
therapeutic
drug
monitoring
for
valproic
acid
in
epilepsy
patient's
serum
samples
by
common,
sensitive,
and
accessible
HPLC‐UV
method.
Because
absence
a
suitable
chromophore
structure,
facile,
selective,
cost‐effective
pre‐column
derivatization
designed.
This
catalyst‐free
ultrasound‐assisted
assay
can
accomplish
very
quickly
only
5.0
min
at
mild
temperature
60°C.
2,4ʹ‐Dibromoacetophenone
nonanoic
used
as
derivatizing
agent
internal
standard,
respectively.
effect
sample
pH,
buffer
concentration,
ultrasound
exposure
time,
reaction
temperature,
amount
were
optimized.
proposed
method
exhibited
good
linear
range
5.0–300.0
µg/mL
with
acceptable
correlation
coefficients
0.9981.
limit
detection
low
0.4
µg/mL.
Also,
quantification
reported
1.3
Interday
intraday
relative
standard
deviations
(
n
=
10)
1.1%
0.3%–7.0%,
In
addition,
recovery
ranged
from
100.3%
to
107.7%.
measurement
performed
presence
several
non‐epilepsy
drugs
developed
protocol.
confirmed
specific
accurate
determination
serum.
A
comparative
evaluation
employed
against
precise
chemiluminescence
immunoassay
approach.
coefficient
between
two
methods
0.9992,
which
demonstrated
results
statistically
same.
Analytica—A Journal of Analytical Chemistry and Chemical Analysis,
Journal Year:
2025,
Volume and Issue:
6(1), P. 8 - 8
Published: March 1, 2025
GLANCE
(Graphical
Layout
Tool
for
Analytical
Chemistry
Evaluation)
is
an
innovative
and
adaptable
free,
editable
template
specifically
designed
to
help
researchers
visually
summarize
their
analytical
chemistry
methods
in
a
structured
clear
manner.
It
provides
accessible
solution
the
challenge
of
presenting
complex
scientific
data,
offering
significant
advantage
over
traditional
reporting
methods,
which
often
lack
visual
clarity.
This
crucial
because
no
previous
tool
has
been
developed
such
comprehensive
concise
format,
significantly
enhancing
process
gathering
key
information,
particularly
review
articles.
The
(bit.ly/409cwDd)
composed
twelve
distinct
attributes,
each
targeting
critical
aspects
method
development
(novelty,
analytes,
sample
preparation,
reagents,
instrumentation,
validation,
matrix
effects
recoveries,
application
real
samples,
metrics,
main
results,
limitations,
additional
information).
By
filling
out
block
with
keywords
or
short
phrases,
authors
can
provide
yet
thorough
overview
method.
Once
completed,
be
easily
downloaded
included
straightforward
integration
enhances
both
clarity
accessibility
publications,
providing
community
quick
snapshot
principal
features
research.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 26, 2025
The
violet
innovation
grade
index
(VIGI)
is
a
pioneering
metric
designed
to
evaluate
the
degree
of
in
analytical
methods.
This
study
introduces
VIGI
tool
(https://bit.ly/VIGItool)
and
demonstrates
its
application
assessing
innovative
potential
various
techniques.
integrates
ten
distinct
criteria─sample
preparation
instrumentation,
data
processing
software,
white
chemistry
derivatives,
regulatory
compliance,
materials
reagents,
miniaturization,
automation,
interdisciplinarity,
sensitivity,
approach─providing
comprehensive
evaluation
that
complements
existing
green,
blue,
red
metrics.
Each
method
assessed
using
survey-based
approach,
resulting
star-shaped
decagon
pictogram
visually
represents
score.
was
successfully
applied
five
case
studies,
revealing
insights
into
strengths
weaknesses
each
terms
innovation.
Methods
incorporating
advanced
materials,
miniaturized
devices,
automation
scored
highly,
reflecting
their
cutting-edge
contributions
chemistry.
Conversely,
methods
lacking
or
interdisciplinary
applications
lower,
highlighting
areas
for
improvement.
work
underscores
importance
prioritizing
metrics
like
development
ensure
remains
at
forefront
scientific
advancement
through
more
effective
sustainable
practices.
BMC Chemistry,
Journal Year:
2025,
Volume and Issue:
19(1)
Published: Jan. 18, 2025
Abstract
White
and
Green
Analytical
Chemistry
are
innovative
approaches
in
analytical
chemistry
that
prioritize
both
sustainability
efficiency.
Together,
these
aim
to
advance
scientific
research
while
minimizing
environmental
impact
enhancing
safety.
This
integration
of
consciousness
into
practices
represents
a
significant
step
forward
achieving
sustainable
progress.
In
the
present
study,
sensitive
eco-friendly
HPLC-DAD
method
was
carried
out
validated
allow
concurrent
determination
Donepezil
HCl
(DPZ)
Curcumin
(CUR)
their
pure
form
laboratory
made
nano-liposome
formulation.
Optimum
seperation
accomplished
by
utilising
Zobrax
Eclipse
Plus
C18
column
(4.6*100
mm,5
μm)
with
gradient
elution
mobile
phase
composed
0.02
M
phosphate
buffer
at
pH
3.2
ethanol
flow
rate
1.5
ml/min.
A
diode
array
detector
(DAD)
implemented
for
detection
273
nm
435
DPZ
CUR,
respectively,
oven
set
40
°C.
The
according
ICH
specifications
terms
accuracy,
precision,
linearity
range,
quantification
limit.
calibration
plots
were
linear
correlation
coefficients
(r2)
=
0.999
over
range
(0.1–100
µg/ml)
successively.
approach
adopted
analyse
medications
prepared
nano-liposomal
formulation
which
analytes
successfully
quantified
good
recovery
values
no
disrubtion
from
added
excipients.
investigation
whiteness,
blueness,
greenness
metrics
revealed
major
benefit
suggested
previous
reported
ones.
Biomedical Chromatography,
Journal Year:
2025,
Volume and Issue:
39(3)
Published: Feb. 7, 2025
Upon
the
development
of
biosimilar
drugs,
structural,
physical-chemical,
and
functional
similarity
to
original
drug
should
be
ensured.
We
have
proposed
a
simple
rapid
HPLC-UV
method
for
assessing
content
C-terminal
Arg166
in
darbepoetin
alfa
samples,
particular
after
their
isolation
from
culture
fluid.
All
stages
described
(from
sample
extraction
obtaining
chromatographic
profile)
take
about
5
h.
The
approach
will
useful
laboratories
developing
technology
producing
recombinant
alfa,
assess
quality
product,
subsequent
optimization
production
process.
New Journal of Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
DNA
methylation
plays
a
critical
role
in
the
epigenetic
regulation
of
gene
expression,
and
5-methylcytosine
(5mC)
is
one
most
important
biomarkers
this
process.
