Dietary Curcumin Nanoparticles Improve Growth Performance, Oxidative Status and Immune Response of European Seabass (Dicentrarchus Labrax) DOI Open Access

Albandari Bin‐Ammar,

Ahmad Ahmeda,

Mouadh Abdelkarim

et al.

Annals of Animal Science, Journal Year: 2024, Volume and Issue: 24(3), P. 889 - 900

Published: Feb. 29, 2024

Abstract A 90 days of feeding trial was employed for evaluating the effects dietary supplementation with curcumin nanoparticles (CURNPs) on growth performance, hematological and biochemical parameters, immune response, antioxidant status, intestinal hepatic architecture in European seabass ( Dicentrarchus labrax ) fingerlings. Fish (n = 300, initial body weight 10.02 g ± 0.11 g) received supplementary CURNPs at rates 0 (control; CURNPs0), 50 (CURNPs50), 100 (CURNPs100) 150 (CURNPs150) mg/kg diet days. Growth performance (final (FBW), daily gain (DWG), (WG), relative rate (RGR), protein efficiency ratio (PER)) feed uptake (feed conversion (FCR)) were improved significantly (100 mg/ kg)-supplemented groups. The parameters red blood corpuscles (RBCs), hemoglobin (Hb), packed cell volume (PCV) (P<0.001) CURNPs150 CURNPs100 groups, while had lowest values monocytes (P 0.019) heterophils (P<0.005) when compared other Glucose higher lipid profile (total cholesterol triglycerides) lower all CURNPs-supplemented groups as to control group. fed CURNPS various levels exhibited remarkable increase digestive enzymes (amylase lipase), markers (immunoglobulin G (IgG), lysozyme phagocytic indices), (superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx)). noticeable decrease (P<0.05) malondialdehyde (MDA) considerable upregulation insulin-like factor 1 IGF1 interleukin-1beta IL-1β mRNA found fish a dose-dependent manner. down regulation heat shock 70 HSP70 observed CURNPs150. Intestinal architectures by CURNPs. In conclusion, consumption digestion, markers, redox homeostasis D. . Therefore, could be supplemented diets natural alternative promote overall performance.

Language: Английский

The effects of nano-curcumin on growth performance, feed utilization, blood biochemistry, disease resistance, and gene expression in European seabass (Dicentrarchus labrax) fingerlings DOI Creative Commons
Samyah D. Jastaniah, Ahd A. Mansour, Ayat H. Al-Tarawni

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 36, P. 102034 - 102034

Published: March 18, 2024

Phytochemicals are used in fish farming to reduce stress and combat diseases during intensification. Recently, nanotechnology has represented a paradigm shift the aquatic feed industry improve solubility, availability, efficacy of phytochemicals. The purpose this research was investigate effects dietary supplementation with nano-curcumin (CUNE) on growth, utilization, body composition, blood biochemistry, antioxidant status, disease resistance Vibrio parahaemolyticus (V. parahaemolyticus), expression insulin-like growth factor 1 (IGF-1), hormone (GH), interleukin-10 (IL-10), interlukin-1β (IL-1β) genes European Seabass (Dicentrarchus labrax) fingerlings. Fish (6.02±0.01) were randomly divided into four equal groups fed varying levels CUNE: 0 (CUNE0), 50 (CUNE1), 60 (CUNE2), 70 mg/kg (CUNE3) for 56 days, respectively. inclusion CUNE significantly improved indices (final weight gain, specific rate) utilization (improved intake lower FCR) seabass fingerlings (p<0.05). However, had no significant effect composition including percentages dry mater, crude protein, lipid, ash (p>0.05). All established an enhancement hematology dose-dependent manner, CUNE3 demonstrating highest values. (70 diet) produced total albumin, globulin, triglycerides, as well lowest glucose, hepatic enzymes (ALT, AST, ALP; p<0.05) compared other groups. Furthermore, CUNE-supplemented showed improvements superoxide dismutase, catalase, glutathione peroxidase, while reducing malondialdehyde Additionally, consumption increased IGF-1, GH, IL-10 manner Interestingly, mg their diets IL-1β gene When challenged V. parahaemolyticus, addition resulted reduced mortality rates. Therefore, could be considered natural additive promote bacteria via supporting serum metabolites, regulation IL-10, IGF-1 tissues

Language: Английский

Citations

15
The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation DOI Creative Commons
Islam M. Saadeldin, Wael A. Khalil, Mona Alharbi

et al.

Animals, Journal Year: 2020, Volume and Issue: 10(12), P. 2281 - 2281

Published: Dec. 3, 2020

Cryopreservation is an essential tool to preserve sperm cells for zootechnical management and artificial insemination purposes. associated with damage via different levels of plasma membrane injury oxidative stress. Nanoparticles are often used defend against free radicals stress generated through the entire process cryopreservation. Recently, or natural nanovesicles including liposomes exosomes, respectively, have shown regenerative capabilities repair damaged during freeze-thaw process. Exosomes possess a potential pleiotropic effect because they contain antioxidants, lipids, other bioactive molecules regulating repairing spermatozoa. In this review, we highlight current strategies using nanoparticles (liposomes exosomes) combat cryoinjuries semen

Language: Английский

Citations

70
Preparation, characterization, and ex-vivo evaluation of curcumin-loaded niosomal nanoparticles on the equine sperm quality during cooled storage DOI
Niloofar Nasiri-Foomani, Mehdi Ebadi, S. Hassani

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130620 - 130620

Published: March 4, 2024

Language: Английский

Citations

11
The beneficial effects of quinoa seed extract supplementation on ram sperm quality following cryopreservation DOI
Wael A. Khalil, Mahmoud A. E. Hassan, Sally Ibrahim

et al.

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 264, P. 107472 - 107472

Published: April 4, 2024

Language: Английский

Citations

9
L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals DOI
Sameh A. Abdelnour, Wael A. Khalil,

Norhan E. Khalifa

et al.

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107429 - 107429

Published: Feb. 9, 2024

Language: Английский

Citations

8
Ramifications of Heat Stress on Rabbit Production and Role of Nutraceuticals in Alleviating Its Negative Impacts: An Updated Review DOI Creative Commons
Tarek A. Ebeid,

Hamad S. Aljabeili,

Ibrahim Al‐Homidan

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(7), P. 1407 - 1407

Published: July 11, 2023

Heat stress has become a widespread concern worldwide, which is major environmental that causes substantial economic loss in the rabbit industry. Compared to other agricultural animals, rabbits are more sensitive heat as they have fewer sweat glands and thicker coat of fur, increasing dissipation complexity. Thus, hurts rabbits’ productivity, meat quality, reproductive performance, antioxidative properties, immune responsiveness, intestinal histomorphology, microbiome. Nutraceuticals include vitamins, minerals, antioxidants, organic acids, fatty probiotics, prebiotics, synbiotics, enzymes, medicinal plants due possible impacts on maintaining common biological situations, strengthening response, preventing illness, ultimately led an increase productivity. recently attracted lot attention alleviate adverse farms. The objective current review provide acquaintance with recent findings about impact productivity advantages dietary supplementation nutraceuticals mitigating it.

Language: Английский

Citations

20
Effect of addition of l‐carnitine to cryopreservation extender on rabbit post‐thaw semen parameters, antioxidant capacity, mitochondrial function, apoptosis and ultrastructure changes DOI
Sameh A. Abdelnour, Mahmoud A. E. Hassan, Ibrahim T. El‐Ratel

et al.

Reproduction in Domestic Animals, Journal Year: 2022, Volume and Issue: 57(8), P. 902 - 911

Published: May 2, 2022

l-Carnitine (LC) is considered to be a natural antioxidant agent that could used improve the efficiency of reproduction. However, precise machinery effect LC supplementation on frozen-thawed rabbit sperm has not been evaluated. Thus, aim this study was evaluate addition freezing medium parameters and ultrastructure changes in sperm. Rabbit bucks (7 months age) were involved, semen collected using artificial vagina method. Pooled cryopreserved tris yolk fructose extender without any supplement (LC0, control group) or with at levels 1, 2 4 mM (LC1, LC2 LC4, respectively). The samples then loaded into 0.25-ml straws frozen over liquid nitrogen vapours before being plunged stored -196°C until evaluation. Data showed significantly increased motility, viability membrane function, while abnormalities decreased (p < .001). Sperm-like apoptosis (early, late necrosis spermatozoa) lower LC4 group compared other groups. enhanced total capacity superoxide dismutase glutathione peroxide activities reduced protein carbonyl malondialdehyde group. Moreover, electron microscopy images demonstrated (2 mM) preserved acrosome plasma protected integrity spermatozoa relation Spermatozoa treated exhibited higher mitochondria potential (MMP) values We conclude (4 quality, capabilities, lipid peroxidation as well MMP activity

Language: Английский

Citations

24
Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation DOI Creative Commons
Sameh A. Abdelnour, Mahmoud A. E. Hassan, Ahmed M. Shehabeldin

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: June 30, 2023

Buffalo sperm is sensitive to cryoinjuries, thus improving cryoresistance a critical approach for wide spreading the assisted reproductive technologies in buffalo. The intention of this work was assess effect propolis-loaded nanoliposomes (PRNL) supplementation semen extender on quality, antioxidant status and some apoptotic genes cryopreserved buffalo semen. PRNL were prepared using cholesterol (Chol) as well soybean lecithin their physicochemical properties characterized. Egyptian bulls (4-6 years) involved, samples collected artificial vagina method. pooled (n = 25 ejaculates) tris containing at 0 (PRNL0), 2 (PRNL2), 4 (PRNL4) 6 µg/mL (PRNL6), respectively. had size 113.13 nm negative zeta potential (- 56.83 mV). Sperm progressive motility, viability, membrane integrity, abnormalities, chromatin damage, redox status, apoptosis investigated after post-thawed Using or significantly increased while abnormalities percentage damages lowest PRNL2 group. Moreover, group exhibited best results all antioxidative activities (TAC, SOD, GPx CAT) with higher levels than other groups (P < 0.05). ROS MDA lower PRLN2 compared groups. caspase 3 enzyme showed values followed by PRNL4 PRNL6 significant differences control. Adding freezing media reduced such Bax Caspase sperm, increase Bcl2 expression control 0.001). Bcl2, not affected addition > electron micrography descriptions exemplified that fortification maintained acrosomal plasma integrities sustained ultrastructure integrity spermatozoa when group, whereas demonstrated highest injury acrosome membranes. Results show enhanced quality via boosting indices, diminishing oxidative stress frozen-thawed sperm.

Language: Английский

Citations

15
Effect of olive, flaxseed, and grape seed nano-emulsion essential oils on semen buffalo freezability DOI
Wael A. Khalil, Mahmoud A. E. Hassan, Kandil Abdel Hai Ali Attia

et al.

Theriogenology, Journal Year: 2023, Volume and Issue: 212, P. 9 - 18

Published: Aug. 30, 2023

Language: Английский

Citations

15
Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa DOI Creative Commons
Wael A. Khalil, Mahmoud A. E. Hassan,

M. El-Harairy

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(18), P. 2973 - 2973

Published: Sept. 20, 2023

Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like morphological changes frozen-thawed buffalo sperm, as well fertilizing capacity. Semen was collected from bulls, diluted (1:10; semen/extender), divided into five aliquots comprising various concentrations 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), 50 (TQNP50) µg/mL, then cryopreserved stored liquid nitrogen (-196 °C). The results revealed that TQNPs (25 to µg/mL) provided most optimal terms membrane integrity (p < 0.001) progressive motility 0.01). contrast, TQNP50 resulted a greater post-thawed viability = 0.02) compared with other groups. addition extender had no discernible morphology measures. Sperm kinematic motion significantly improved group control effectively reduced content H2O2 MDA levels total antioxidant capacity extended semen increased number intact acrosomes 0.0001) decreased exocytosed 0.0001). A significant reduction observed non-return rates cows inseminated TQNP50-treated spermatozoa were higher than those 0.05; 88% vs. 72%). These findings suggested freezing supplemented could enhance cryotolerance fertility sperm.

Language: Английский

Citations

14