Developing an eDNA approach for wetland biomonitoring: Insights on technical and conventional approaches
Starsha Bird,
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Paul Edward Dutton,
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Shaun Wilkinson
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et al.
Environmental DNA,
Journal Year:
2024,
Volume and Issue:
6(3)
Published: May 1, 2024
Abstract
Wetlands
are
ecologically
and
culturally
significant
ecosystems
that
experiencing
biodiversity
declines
globally.
Biomonitoring
techniques
use
environmental
DNA
(eDNA)
to
detect
monitor
well
established
in
lake,
riverine,
marine
ecosystems.
However,
their
wetlands
requires
further
development
due
the
presence
of
sediments
block
eDNA
filters
limit
water
filtration,
alongside
a
lack
standardized
methodology.
In
this
study,
we
examined
dynamics
understand
spatiotemporal
patterns
an
Aotearoa
New
Zealand
wetland
optimize
application
wetland‐specific
challenges.
We
sampled
four
sites
across
Opuatia
Wetland
at
three
time
points
during
austral
spring.
conducted
conventional
taxonomic
surveys,
tested
different
filter
sizes
(1.2
μm,
5
semi‐quantitative
dacron
filters),
assessed
our
ability
foreign
(from
kea;
Nestor
notabilis
)
distances
post‐release.
found
differences
sequence
composition
space,
when
using
sized
filters.
data
generally
complemented
(versus
replaced)
survey
identification
methods,
with
certain
species
only
detected
by
one
method
or
other.
Taxonomic
resolution
sampling
methods
often
exceeded
eDNA.
Foreign
was
detectable
10
m
from
its
release
point
for
up
1
week
Our
results
provide
new
considerations
future
research
environments,
where
rapid
biomonitoring
needed
support
conservation
preservation.
Language: Английский
Current Utilization and Further Application of Zooplankton Indices for Ecosystem Health Assessment of Lake Ecosystems
Sustainability,
Journal Year:
2023,
Volume and Issue:
15(14), P. 10950 - 10950
Published: July 12, 2023
For
the
sustainable
use
of
lake
ecosystem
services—water
resources,
aquatic
habitats
for
biodiversity
conservation,
and
aesthetic
values
as
waterfront
space—ecosystem
health
assessments
using
biota
are
implemented
important
national
environmental
monitoring
projects.
Zooplankton
play
a
key
role
an
linkage
in
material
circulation
secondary
producers
ecosystems.
At
same
time,
they
influence
composition
biomass
other
communities
through
biological
interactions.
In
this
review,
we
summarize
useful
zooplankton
indices
assessment
suggest
considerations
their
use.
Suggestions
provided
practical
application
indirectly
measured
biomass,
well
potential
limitations
eDNA
application,
which
has
recently
been
actively
utilized
monitoring.
Language: Английский
Climate warming and nutrient enrichment destabilize plankton network stability over the past century
Communications Earth & Environment,
Journal Year:
2025,
Volume and Issue:
6(1)
Published: March 29, 2025
Language: Английский
Benefits of combined environmental DNA and microscopy for diversity monitoring in rotifer community: A mesocosm experiment
Ecological Indicators,
Journal Year:
2023,
Volume and Issue:
155, P. 110930 - 110930
Published: Sept. 18, 2023
Rotifers
are
crucial
for
the
monitoring
of
aquatic
ecosystems
because
sensitive
response
rotifer
community
structure
to
environmental
changes.
Comparisons
and
combinations
meta-barcoding
(sequence
analysis)
traditional
morphological
identification
responses
rare.
To
better
understand
differences
between
two
approaches,
we
conducted
a
study
using
48
mesocosms
(2500
L)
combination
warming,
nutrient
loading,
pesticide
evaluate
applicability
both
methods
communities
in
order
how
respond
multiple
stressors.
The
findings
demonstrated
that
degree
matching
techniques
was
limited,
despite
fact
there
substantial
positive
association
morphology
abundance
determined
by
sequence
abundances
eDNA
metabarcoding.
Although
had
similar
various
stresses,
analysis
can
identify
rotifers
difficult
with
such
as
Bdelloidea,
while
classical
were
more
suitable
assessing
effects
changes
on
communities.
This
provided
valuable
implications
managing
since
it
enables
us
spot
early
take
necessary
precautions
safeguard
these
ecosystems.
Language: Английский
The effects of water quality parameters, water level changes, and mixing on zooplankton community dynamics in a tropical high-mountain Lake Ardibo, Ethiopia
Environmental Monitoring and Assessment,
Journal Year:
2023,
Volume and Issue:
195(8)
Published: July 10, 2023
Language: Английский
The effects of water quality parameters, water level changes, and mixing on zooplankton community daynamics in a tropical high-mountain Lake Ardibo, Ethiopia.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: March 22, 2023
Abstract
The
diversity
and
abundance
of
zooplankton
community
are
affected
by
the
change
in
physicochemical
parameters,
trophic
interactions,
water
quality,
level
changes,
periodic
mixing.
influence
changes
mixing
on
distribution
was
seasonally
studied
for
Lake
Ardibo
from
October
2020
to
September
2021
collected
3
sites.
Results
variables
indicated
that
all
variables,
except
turbidity,
varied
significantly
(
p
<
0.05)
sampling
seasons.
A
total
36
species
were
recorded,
comprising
18
rotifers,
11
cladocerans,
7
cyclopoid
copepods.
Zooplankton
with
peak
(4232.13
ind.
L
−1
)
recorded
dry
season
lowest
numbers
(402.42
during
long
rainy
period.
Redundancy
analysis
(RDA)
results
dissolved
oxygen,
NH
-N,
NO
-N
conductivity
most
significant
driving
forces
seasonal
successions
communities
whereas
turbidity
has
a
minor
role.
Cyclopoid
copepod
<0.05)
higher
season,
which
could
be
correlated
partial
(atelomixis)
phenomenon
occurred
Language: Английский