Effect of Fish Meal Substitution with Black Soldier Fly (Hermetia illucens) on Growth Performance, Feed Stability, Blood Biochemistry, and Liver and Gut Morphology of Siamese Fighting Fish (Betta splendens)

Aquaculture Nutrition, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 15

Published: June 20, 2023

Insects such as black soldier fly larvae (BSFL) are gaining interest among researchers and the aquafeed industry due to fluctuating price supply of fish meal (FM). This study evaluated growth performance, feed stability, blood biochemistry, liver gut morphology Betta splendens using BSFL an alternative FM. Five formulated diets were prepared: 0% BSFL, 6.5% 13% 19.5% 24.5% BSFL. The expansion rate, pellet durability index, floatability, bulk density, water stability prepared have been assessed. Except for diameter feed, all parameters studied differed significantly (

Language: Английский

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Effects of Dietary Plant Protein Replacement with Insect and Poultry By-Product Meals on the Liver Health and Serum Metabolites of Sea Bream (Sparus aurata) and Sea Bass (Dicentrarchus labrax)

Animals, Journal Year: 2024, Volume and Issue: 14(2), P. 241 - 241

Published: Jan. 12, 2024

The liver health of Gilthead sea bream and European bass, fed with fish meal-free diets, including various proportions plant proteins, as well insect poultry by-product meals, was investigated through biochemical histological analyses using a new index (LI) formula. Four isoproteic (45% Dry Matter, DM) isolipidic (20% diets were compared, plant-based control diet (CV) three other test in which 40% protein-rich ingredient mixture replaced meals from

Language: Английский

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The impact of diets containing Hermetia illucens meal on the growth, intestinal health, and microbiota of gilthead seabream (Sparus aurata)

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 50(3), P. 1003 - 1024

Published: Feb. 22, 2024

Abstract The present study investigated the effect of replacing fishmeal (FM) with insect meal Hermetia illucens (HI) in diet Sparus aurata farmed inshore on growth, gut health, and microbiota composition. Two isolipidic (18% as fed) isoproteic (42% diets were tested at farm scale: a control without HI an experimental 11% FM. At end 25-week feeding trial, final body weight, specific growth rate, feed conversion hepatosomatic index not affected by diet. Gross morphology gastrointestinal tract liver was unchanged showed no obvious signs inflammation. High-throughput sequencing 16S rRNA gene amplicons (MiSeq platform, Illumina) used to characterize microbial community profile that Proteobacteria, Fusobacteria, Firmicutes dominant phyla gilthead seabream, regardless Dietary inclusion altered significantly decreasing abundance Cetobacterium increasing relative Oceanobacillus Paenibacillus genera. Our results clearly indicate alternative animal protein source positively affects seabream beneficial genera, thereby improving health maintaining performance S. from coastal farms.

Language: Английский

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Defatted Black Soldier Fly Meal in Diet for Grow-Out Gilthead Seabream (Sparus aurata L. 1758): Effects on Growth Performance, Gill Cortisol Level, Digestive Enzyme Activities, and Intestinal Histological Structure

Aquaculture Research, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 18

Published: March 16, 2023

A trial was performed to study the effects of a partially defatted Hermetia illucens (L. 1758) larvae meal (HIM) replace fish in diet an adult gilthead sea bream. The were fed for 120 days with diets containing HIM: 0% (CTRL), 25 (HI25), 50 (HI50), and 75% (HI75), corresponding inclusion levels 0, 9.2, 18.4, 27.6%. digestibility carried out evaluate apparent coefficients (ADC). Growth performance, morphometric biometric indexes, gill cortisol, status gut mucosa, enzymes’ (alkaline proteases, lipase, amylase) activity evaluated. Morphometric digestibility, enzymes not affected by diet. Conversely, 27.6% HI level worsened specific growth rate, feed conversion ratio, protein efficiency ratio. Histology showed no significant differences between CTRL HI25 groups. many HI50 HI75 fish, anatomo-functional changes observed. No statistical found among ADC dry matter, crude protein, ether extract, gross energy. results that HIM can be used as alternative source up 18.4% without impacting ADCs, enzymes.

Language: Английский

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Insect meal in aquafeeds: A sustainable path to enhanced mucosal immunity in fish

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 150, P. 109625 - 109625

Published: May 12, 2024

The mucosal surfaces of fish, including their intestines, gills, and skin, are constantly exposed to various environmental threats, such as water quality fluctuations, pollutants, pathogens. However, cells microbiota closely associated with these work in tandem create a functional protective barrier against conditions. Recent research has shown that incorporating specific feed ingredients into fish diets can significantly boost general immune response. Among the being investigated, insect meal emerged one most promising options, owing its high protein content immunomodulatory properties. By positively influencing structure function surfaces, potential enhance overall status fish. This review provides comprehensive overview benefits aquafeed ingredient for augmenting response

Language: Английский

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Defatted black soldier fly (Hermetia illucens) diets improved hemato-immunological responses, biochemical parameters, and antioxidant activities in Streptococcus iniae-infected Nile tilapia (Oreochromis niloticus)

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 25, 2025

Abstract Background Challenges of limited supply and increasing prices fishmeal have driven the aquaculture nutritionists to seek alternative sustainable protein rich ingredients keep manufacturing aquafeeds in a maintainable cost-effective way. Black soldier fly, Hermetia illucenslarvae meal represent great potential as aquafeeds. Methods Three replacement diets for were prepared at different levels defatted black fly (Hermetia illucens) (DBSFM): Diet 1 (0 g DBSFM /kg diet, control), 33% (DBSFM-33%, 66 diet), 100% (DBSFM-100%, 200 diet) investigate their effects on biochemical parameters, immuno-hematological responses, antioxidant activities, inflammatory gene expression Nile tilapia, Oreochromis niloticus , total 270 (40.0 ± 0.50 g) before after challenge with Streptococcus iniae ( S. ). The feeding trial lasted six weeks (pre-challenge) two (post-challenge). Results results showed significant improvement white blood cell count P < 0.01), lymphocyte serum lysozyme activity 0.001), phagocytic mostly DBSFM-100% group following pre-challenge phase compared control group. Post-challenge exhibited increases indices DBSFM-treated groups Following pre- post-challenge periods, both DBSFM-supplemented experienced 0.01, levels. Albumin globulin also similar 0.05, but only post-challenge. Total capacity increase post-challenge, did superoxide dismutase, catalase, glutathione peroxidase liver spleen. Conversely, glucose, cortisol, malondialdehyde followed opposite trend. inclusion revealed 0.05) up-regulation interleukin 1β (IL-1β) control, no significance > was seen other genes. Anti-inflammatory-related genes transforming growth factor -β interleukin-10 mRNA up-regulated IL-1β tumor necrosis factor- α. Conclusion These findings suggest that tilapia challenged may experience enhancements hemato-immunological capability, anti-inflammatory when fish is replaced up 100%.

Language: Английский

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Genetic selection for high growth improves the efficiency of gilthead sea bream (Sparus aurata) in using novel diets with insect meal, single-cell protein and a DHA rich-microalgal oil

Aquaculture, Journal Year: 2023, Volume and Issue: 578, P. 740034 - 740034

Published: Aug. 30, 2023

Genetic selection and novel raw materials for aquafeeds are current key tools in the ongoing effort to increase productivity, efficiency, sustainability of aquaculture sector. Selective breeding could also improve utilization dietary formulations with emergent ingredients. Gilthead sea bream juveniles, either coming from a selective program based on growth traits, or non-selected population, were nutritionally challenged two that compared Control diet 15% FM 6% FO commercial levels this species. The included an insect meal (INS) at 5% replace 33.3% FM, single-cell protein (SCP) 10% 66.7% FM. Fish oil was totally replaced these diets by blend poultry Veramaris algal oil. Better feed selected genotype fish observed, any assayed. INS SCP reduced general performance reducing intake. However, fed showed very similar lower conversion ratio control diet. increased n-3 LC-PUFA tissues, particularly DHA, irrespective genotype, as result inclusion DHA-rich microalgal Neither genetic nor use affected fillet proximate composition consequently, quality terms texture sensorial perception consumers. Overall, results reaffirm positive effects programs improving productive indicators, well support formulations, using H. illucens, M. capsulatus partial replacers gilthead (33 66% replacement, respectively), oils total replacer FO.

Language: Английский

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Physiological Response of European Sea Bass (Dicentrarchus labrax) Juveniles to an Acute Stress Challenge: The Impact of Partial and Total Dietary Fishmeal Replacement by an Insect Meal

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 815 - 815

Published: May 14, 2024

This study aimed to explore the effect of FM substitution by defatted Tenebrio molitor larvae meal (dTM) on response European seabass an acute stress challenge. An FM-based diet was used as a control and two other isoproteic/isoenergetic diets were formulated replace 50 100% dTM. Each tested in quadruplicate groups 15 fish (69 ± 5 g) fed until visual satiety for 16 weeks. After feeding trial, subjected 1 min air exposure followed h recovery before sampling. The haematological profile, plasma metabolites, humoral immunity biomarkers, well hepatic oxidative antioxidant capacity, analysed. A clear observed significant increase haemoglobin, haematocrit, red blood cells, almost all evaluated metabolites parameters, regardless dietary treatment. obtained results demonstrated that partial IM did not affect seabass. However, total replacement increased activity peroxidase superoxide dismutase TM100.

Language: Английский

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Effects of replacing fishmeal with Spirulina platensis powder on the Zig-zag eel (Mastacembelus armatus): Growth performance, flesh quality, digestibility, and gut microbial composition

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 37, P. 102263 - 102263

Published: July 29, 2024

Language: Английский

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Effect of Graded Inclusion of Black Soldier Fly (Hermetia Illucens, Linnaeus, 1758) Meal in Diets for Gilthead Seabream (Sparus Aurata, Linnaeus, 1758) on Gut Microbiome and Liver Morphology.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

Over the last decades, insect meal has received great attention for finfish diets, due to its nutritional composition and low ecological footprint. In present study, we assessed response of gut microbiota liver histology gilthead seabream (Sparus aurata) fed four experimental diets including black soldier fly (Hermetia illucens) (HI) used replace 0 (HI0), 25 (HI25), 35 (HI35) 50 (HI50) percent fish in a 131-day feeding trial. At end experiment, remarkable change related HI inclusion was observed, with preponderance Nitrospirota in HI0 HI25 Chloroflexi HI35 HI50 groups. Predictive analysis on bacterial metabolic pathways showed clear separation between HI0-HI25 HI35-HI50 The shifts observed suggest pivotal role inducing bacterial-mediated physiological this species, probably chitin content fatty acid profile ingredient. Liver higher hepatocyte size from group, while marginal adaptive group. Overall, 50% dietary is not recommended, since possible alteration lipid deposition, particularly at hepatic level, were highlighted species.

Language: Английский

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