Comparative proteomic analysis reveals the response mechanism of freshwater leech (Whitmania pigra) under heat-stress challenge DOI

Shuaibing Wang,

Xiaochuan Zheng, Quan Wang

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 54, P. 101406 - 101406

Published: Dec. 22, 2024

Language: Английский

Physiological analysis and transcriptome sequencing revealed that HSPA1 was involved in response to heat stress in thick-shell mussels, Mytilus coruscus DOI
Huajian Lin,

Ronghui Yao,

Sisi Wei

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: 55, P. 101449 - 101449

Published: March 1, 2025

Language: Английский

Citations

0
Transcriptomic analysis reveals the survival strategies of Mytilus coruscus under short-term rising seawater temperatures DOI

Bingqi Wei,

Hao Wang, Zhenyu Dong

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: 55, P. 101481 - 101481

Published: March 12, 2025

Language: Английский

Citations

0
Repeated marine heatwaves aggravate the adverse effects of nano-TiO2 on physiological metabolism of the thick-shelled mussel Mytilus coruscus DOI

Shuaishuai Wei,

Yiwei Yin,

Zhihan Tu

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 364, P. 143260 - 143260

Published: Sept. 1, 2024

Language: Английский

Citations

3
Novel Ser74 of NF-κB/CgIκBα Phosphorylated by MAPK/ERK Regulates Temperature Adaptation in Oysters DOI Creative Commons
Chaogang Wang,

Zhuxiang Jiang,

Mingyang Du

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Abstract Phosphorylation of IκBα at Ser32 and Ser36 by IKKs during biotic stress triggers its ubiquitin-proteasome degradation, causing to the nuclear translocation REL, representing a key cascade mechanism in metazoans conserved immune core signaling pathway, NF-κB. However, studies on response abiotic signal transduction phosphorylation mollusks are lacking. Here, we firstly report novel heat-induced site (Ser74) major NF-κB/ Cg oysters, phosphorylated MAPK/ ERK1/2, which independently mediated subsequent degradation without decreased thermal stability. The promoted REL translocation, stimulated cell survival related gene expression defend against stress. MAPK NF-κB pathways exhibited stronger activation patterns higher environmental temperature warm-adapted Crassostrea angulata than those cold-adapted C. gigas -two allopatric congeneric oyster species with differential habitat temperatures. These findings unveil complex unique phosphorylation-mediated mechanisms marine invertebrates, further expand our understanding evolution function established classical pathway crosstalk mechanisms.

Language: Английский

Citations

0
MAPK/ERK Phosphorylates Conserved PK Ser11 Site in Mollusk Regulates Divergent Thermal Metabolism of Two Congeneric Oyster Species DOI
Chaogang Wang,

Zhuxiang Jiang,

Mingyang Du

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Digestive Enzyme Activities in Mussel Mytilus californianus Endure Acute Heat Exposure in Air DOI

Daniel R. Rankins,

Kwasi Connor, Emily E. Bryant

et al.

Integrative and Comparative Biology, Journal Year: 2024, Volume and Issue: unknown

Published: June 9, 2024

Synopsis The mussel Mytilus californianus is an ecosystem engineer forming beds along the coastlines of Northeastern Pacific shores. As sessile organisms, they modulate their energy balance through valve movements, feeding, and digestive functionality. A recent study observed that activity enzyme cellulase was higher than predicted in mussels high on shore, where temperatures are characteristically food availability limited compared to low-shore habitats. In current study, we this scavenging behavior induced mitigate losses related heat-shock responses—that amylase will display hyperactivity for recourses face aerial heating. laboratory, acclimated three complex diets differed starch cellulose composition, followed by two acute heat shocks (+8°C) laboratory. Results showed no heated mussels. These results differ from previous studies lowered following acclimation. This difference across heat-stress exposure time important when analyzing bed disturbances waves compromise or cause death within adult populations.

Language: Английский

Citations

0
MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species DOI Creative Commons
Chaogang Wang, Mingyang Du,

Zhuxiang Jiang

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(7), P. 110321 - 110321

Published: June 20, 2024

Pyruvate kinase (PK), as a key rate-limiting enzyme in glycolysis, has been widely used to assess the stress tolerance and sensitivity of organisms. However, its phosphorylation regulatory mechanisms mainly focused on human cancer research, with no reports marine In this study, we firstly reported conserved PK Ser11 site mollusks, which enhanced activity by promoting substrate binding, thereby regulating divergent thermal metabolism two allopatric congeneric oyster species differential habitat temperature. It was phosphorylated ERK kinase, regulated classical MAPK pathway. The MAPK/ERK-PK signaling cascade responded increased environmental temperature exhibited stronger activation pattern relatively thermotolerant (

Language: Английский

Citations

0
Novel Ser74 of NF-κB/IκBα phosphorylated by MAPK/ERK regulates temperature adaptation in oysters DOI Creative Commons
Chaogang Wang,

Zhuxiang Jiang,

Mingyang Du

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 11, 2024

Phosphorylation of Ser32 and Ser36 controls the degradation IκBα is conserved cascade mechanisms immune core signaling pathway, NF-κB pathway in metazoans, but it's response to abiotic stress presence novel phosphorylation other species remain unclear. Herein, we reported a heat-induced site (Ser74) at oysters' major IκBα, which independently regulated ubiquitination-proteasome without requirement S32 S36. And this was phosphorylated by ERK/MAPK then promoted REL nuclear translocation activate cell survival related genes defend heat-stress. The MAPK-NF-κB exhibited divergent thermal responses adaptation patterns between two congeneric oyster with differential habitat temperatures, indicating its involvement shaping temperature adaptation. This study demonstrated that existence complex unique phosphorylation-mediated transduction mechanism marine invertebrates, expanded our understanding evolution function established classical crosstalk mechanisms.

Language: Английский

Citations

0
Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis DOI
Meimei Liu, Zhichao Wang,

Hongwei Ni

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 40, P. 102559 - 102559

Published: Dec. 10, 2024

Language: Английский

Citations

0
Comparative proteomic analysis reveals the response mechanism of freshwater leech (Whitmania pigra) under heat-stress challenge DOI

Shuaibing Wang,

Xiaochuan Zheng, Quan Wang

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 54, P. 101406 - 101406

Published: Dec. 22, 2024

Language: Английский

Citations

0