Genome-wide discovery of underlying genetic factors associated with fresh and frozen-thawed semen traits in composite ram breeds exhibiting different cryotolerance

Cryobiology, Journal Year: 2025, Volume and Issue: 118, P. 105197 - 105197

Published: Jan. 18, 2025

Language: Английский

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Determinant genetic markers of semen quality in livestock

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 4, 2024

The reproductive efficiency of livestock is crucial for agricultural productivity and economic sustainability. One critical factor in successful fertilization the viability offspring quality semen. Poor semen quality, especially frozen-thawed used artificial insemination (AI) have been shown to influence conception outcomes, resulting a negative impact on production. Recent advancements genetic research identified specific markers linked traits various species, such as cattle, sheep, goats, pigs, buffalo, equines. These are essential screening males breeding suitability, which turn enhances selective programs. Understanding these improving performance increasing populations. This review offers comprehensive overview associated with key livestock. It explores underlying mechanisms their practical implications animal management. underscores importance integrating insights into strategies optimize ensure sustainable development industries.

Language: Английский

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Genetic parameters and genome-wide association studies including the X chromosome for various reproduction and semen quality traits in Nellore cattle

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 10, 2025

Abstract Background The profitability of the beef industry is directly influenced by fertility rate and reproductive performance both males females, which can be improved through selective breeding. When performing genomic analyses, genetic markers located on X chromosome have been commonly ignored despite being one largest chromosomes in cattle genome. Therefore, primary objectives this study were to: (1) estimate variance components parameters for eighteen male five female traits Nellore including analyses; (2) perform genome-wide association studies functional analyses to better understand background cattle. Results percentage total direct heritability (h 2 ) explained x ranged from 3 32% (average: 16.4%) 9 67% 25.61%) traits, respectively. Among related breeding soundness evaluation, overall bull semen evaluation quality accounted highest proportion h relative with an average 39.5% 38.75%, number significant per trait 7 (seminal vesicle width) 43 (total major defects). zero five. A 683, 252, 694, 382, 61, 77 genes overlapped regions identified performance, quality, morphology, defects, bulls’ key candidate are PRR32 , STK26 TMSB4X TLR7 PRPS2 SMS SMARCA1 UTP14A BCORL1 . main gene ontology terms “Oocyte Meiosis”, “Progesterone Mediated Oocyte Maturation”, “Thermogenesis”, “Sperm Flagellum”, “Innate Immune Response”. Conclusions Our findings indicate role phenotypic variability reproduction Breeding programs aiming improve these should consider adding information their analyses.

Language: Английский

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Genetic diversity and selection signatures in sheep breeds

Journal of Applied Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Abstract Natural and artificial selection in domesticated animals can cause specific changes genomic regions known as signatures. Our study used the integrated haplotype score (iHS) Tajima’s D tests within non-overlapping windows of 100 kb to identify signatures, addition genetic diversity linkage disequilibrium estimates 9498 sheep from breeds Ireland (Belclare, Charollais, Suffolk, Texel, Vendeen). The mean observed expected heterozygosity for all were 0.353 0.355, respectively. Suffolk had least variation and, along with slower decay. iHS detected signatures breeds, some overlapping, thus forming longer segments Common identified across methods Belclare Texel having several common under positive selection. Several genes signature regions, including ITGA4 , TLR3 TGFB2 related immune system against endoparasites; DLG1 ROBO2 MXI1 MTMR2 CEP57 FAM78B reproductive traits; WDR70 milk SCHM1 MYH15 meat TAS2R4 TAS2R39 TAS2R40 adaptive traits. In conclusion, our results demonstrated moderate characterized harboring associated traits, production, traits such endoparasite resistance.

Language: Английский

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Single nucleotide polymorphism-based analysis of linkage disequilibrium and runs of homozygosity patterns of indigenous sheep in the southern Taklamakan desert

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 18, 2025

Runs of Homozygosity (ROH) are homozygous genomic fragments inherited from parents to offspring. ROH can be used indicate the level inbreeding, as well identify possible signatures artificial or natural selection. Indigenous sheep populations on southern edge Taklimakan Desert have evolved unique genetic traits adapted extreme desert environments. In an attempt better understand adaptive mechanisms these under harsh conditions, we Illumina® Ovine SNP50K BeadChip perform a characterization three recognized breeds (Duolang: n = 36, Hetian: 84, Qira black: 189) and one ecotypic breed (Kunlun: 27) in region. Additionally, assessed inbreeding coefficients through analysis, revealing insights into history populations. Subsequently, retrieved candidate genes associated with economic islands each breed. To autozygosity distribution indigenous relative international breeds, also included commercial mutton (Poll Dorset: 108, Suffolk: 163, Texel: 150). The study revealed that among seven Hetian exhibited shortest linkage disequilibrium (LD) decay distance, while Kunlun demonstrated highest LD levels. A total 10,916 ROHs were obtained. number per ranged 34 (Kunlun) 2,826 (Texel). length was mainly 1–5 Mb (63.54%). Furthermore, 991 specific identified, including those heat tolerance, adaptability, energy metabolism, reproduction, immune response. These findings elucidate adaptation Desert, uncovering distinctive characteristics formation, advocating for conservation enhancement local

Language: Английский

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The Effect of Curcuma Extract on the Membrane and Acrosome Integrity of Spermatozoa in Buck Fresh Semen

IOP Conference Series Earth and Environmental Science, Journal Year: 2025, Volume and Issue: 1476(1), P. 012054 - 012054

Published: April 1, 2025

Abstract The objective of this research was to assess the impact curcuma extract on enhancing quality buck sperm. Observations were carried out microscopically, namely by observing membrane and acrosome integrity spermatozoa in fresh semen. materials that will be used are semen extract. employed a non-factorial experimental design. Completely Randomized Design with five treatments replications. treatment given addition 0ml, 25 ml, 50ml, 75ml 100ml results study showed administration had significant effect acrosomal spermatozoa. It can concluded increase percentage membranes

Language: Английский

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Genome-Wide Association Study (GWAS) on Reproductive Seasonality in Indigenous Greek Sheep Breeds: Insights into Genetic Integrity

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(4), P. 279 - 279

Published: April 16, 2025

A key feature in sheep biology is reproduction seasonality which concerns the cyclical occurrence of natural breeding, therefore does not take place throughout year. Since are short-day breeders, amount daylight has an impact on their reproductive activity. The melatonin receptor subtype 1A (MTNR1A) gene primary that been linked with seasonality. Nonetheless, information regarding potential genetic association between other loci and scarce. Genome-wide study (GWAS) considered a cutting-edge methodology for comprehending architecture complex traits since it enables discovery many markers to different features. In present study, three indigenous Greek breeds were investigated using GWAS—two presented strict patterns seasonality, i.e., Florina Karagkouniko breeds, while third one, Chios breed had ability exhibit estrus year—in attempt detect All animals purebred originating from national stationary stock, whereas originated commercial farm. Interestingly, significant differentiation stock groups was suggested by principal component analysis, phylogenetic admixture spatial point patterns, these two being less heterogeneous. This finding highlights value stocks towards maintenance integrity sheep, demonstrating station’s critical role conservation native breeds. On hand, according GWAS data no correlated emphasizing MTNR1A as main determinant non genetically improved

Language: Английский

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Impact of melatonin administration on sperm quality, steroid hormone levels, and testicular blood flow parameters in small ruminants: A meta-analysis

Veterinary World, Journal Year: 2024, Volume and Issue: unknown, P. 911 - 921

Published: April 1, 2024

Background and Aim: The impact of exogenous melatonin on the sperm quality small ruminants is controversial. Therefore, this study aimed to synthesize previous findings influence injection quality, steroid hormones, testicular blood flow in ruminants. Materials Methods: Thirty studies were analyzed by computing raw mean difference (RMD) as effect size between control treatment groups, using inverse variance for random-effect model method moments DerSimonian Laird. We assessed heterogeneity among Q test. I2 statistic was used classify observed heterogeneity. Egger’s regression indicate publication bias. Results: Melatonin (p < 0.05) affected concentration (RMD = 0.42 × 109/mL), morphology 2.82%), viability 2.83%), acrosome integrity 4.26%), DNA 1.09%). Total motility 5.62%), progressive 7.90%), 8.68%), 2.01%) post-thawed semen melatonin-treated group also increased 0.05). Similarly, with enhanced total 5.78%), 5.28%), curvilinear velocity 4.09 μm/s), straight-line 5.61 average path 4.94 μm/s). Testosterone 1.02 ng/mL) estradiol 17-ß levels 0.84 pg/mL) elevated melatonin-injected group. implantation ameliorated flow, indicated a significant reduction resistive index 0.11) pulsatility –0.15). Conclusion: administration can increase reproductive performance male Keywords: goat, implantation, melatonin, meta-analysis, reproduction, sheep.

Language: Английский

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Genetic Diversity, Selection Signatures, and Genome-Wide Association Study Identify Candidate Genes Related to Litter Size in Hu Sheep

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9397 - 9397

Published: Aug. 29, 2024

Hu sheep is a renowned prolific local breed in China, widely distributed across the country due to its excellent reproductive performance. Deciphering molecular mechanisms underlying high fecundity of crucial for improving litter size ewes. In this study, we genotyped 830 female using Illumina OvineSNP50 BeadChip and performed genetic diversity analysis, selection signature detection, genome-wide association study (GWAS) size. Our results revealed that population exhibits relatively diversity. A total 4927 runs homozygosity (ROH) segments were detected, with majority (74.73%) being short length. Different genomic inbreeding coefficients (

Language: Английский

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A review on candidate genes associated with sheep fertility traits: Implications for genetic improvement of indigenous sheep breeds in developing countries

Ecological Genetics and Genomics, Journal Year: 2024, Volume and Issue: 31, P. 100243 - 100243

Published: April 14, 2024

Language: Английский

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