New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis DOI

Bendong Shi,

Bowen Dong,

Jianhua Shan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(7), P. 3741 - 3754

Published: Feb. 10, 2024

Decabromodiphenyl ether (BDE-209) is a widely used brominated flame retardant that can easily detach from materials and enter into feed foodstuffs, posing serious risk to human animal health food safety of origin. However, the immunotoxic effects BDE-209 on avian spleen exact mechanism toxicity remain unknown. Therefore, we established an experimental model BDE-209-exposed chickens positive control cyclophosphamide-induced immunosuppression in vivo treated MDCC-MSB-1 cells chicken splenic primary lymphocytes with vitro. The results showed treatment caused morphological structural abnormalities spleens. Mechanistically, indicators related oxidative stress, endoplasmic reticulum stress (ERS), autophagy, apoptosis were significantly altered by exposure both lymphocytes, but use N-acetylcysteine or 4-phenylbutyric acid reversed these changes. In addition, decreased antimicrobial peptide immunoglobulin gene expression. conclusion, present research revealed enhanced lymphocyte autophagy via ROS-mediated ERS pathway. This signaling cascade regulatory relationship not only opens up new avenue for studying immunotoxicity also provides important insights preventing hazards health.

Language: Английский

Fisetin may protect early porcine embryos from oxidative stress by down-regulating GRP78 levels DOI Creative Commons

Xiu‐Wen Yuan,

Hao Guo, Chao‐Rui Wang

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19198 - e19198

Published: March 28, 2025

Fisetin is a natural flavonol with variety of biological activities, including anti-inflammatory and antitumor activities. However, the effect fisetin on mammalian oocyte embryo development unknown, so in this study, we used porcine oocytes as an experimental model, added optimal concentrations to vitro culture medium after parthenogenetic activated investigate development. It was found that 0.1 µM significantly increased cleavage rate blastocyst formation rate, quality blastocysts also improved. Staining results showed levels reactive oxygen species (ROS), autophagy, endoplasmic reticulum stress apoptosis were reduced, while glutathione mitochondrial function fisetin-treated group early embryos compared control group. Meanwhile, decreased expression level marker protein GRP78 (0.71 ± 0.19). In addition, genes related pro-apoptosis, autophagy antioxidant, pluripotency mitochondrial. According our results, promotes embryonic porcine, may be realized by down-regulating GRP78.

Language: Английский

Citations

0
New Insights on the Protection of Endangered Aquatic Species: Embryotoxicity effects of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) via integrin-mediated oxidative stress and inflammatory pathways in Siberian sturgeon, Acipenser baerii DOI
Changyuan Li, Yuan Miao,

Jiayi Du

et al.

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110307 - 110307

Published: April 1, 2025

Language: Английский

Citations

0
BDE-47-mediated cytotoxicity via autophagy blockade in 3D HepaRG spheroids cultured in alginate microcapsules DOI Creative Commons
Raul Ghiraldelli Miranda, Gabriel Cicolin Guarache, Anderson H.F.F. Leão

et al.

Chemico-Biological Interactions, Journal Year: 2023, Volume and Issue: 388, P. 110831 - 110831

Published: Dec. 13, 2023

Language: Английский

Citations

1
New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis DOI

Bendong Shi,

Bowen Dong,

Jianhua Shan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(7), P. 3741 - 3754

Published: Feb. 10, 2024

Decabromodiphenyl ether (BDE-209) is a widely used brominated flame retardant that can easily detach from materials and enter into feed foodstuffs, posing serious risk to human animal health food safety of origin. However, the immunotoxic effects BDE-209 on avian spleen exact mechanism toxicity remain unknown. Therefore, we established an experimental model BDE-209-exposed chickens positive control cyclophosphamide-induced immunosuppression in vivo treated MDCC-MSB-1 cells chicken splenic primary lymphocytes with vitro. The results showed treatment caused morphological structural abnormalities spleens. Mechanistically, indicators related oxidative stress, endoplasmic reticulum stress (ERS), autophagy, apoptosis were significantly altered by exposure both lymphocytes, but use N-acetylcysteine or 4-phenylbutyric acid reversed these changes. In addition, decreased antimicrobial peptide immunoglobulin gene expression. conclusion, present research revealed enhanced lymphocyte autophagy via ROS-mediated ERS pathway. This signaling cascade regulatory relationship not only opens up new avenue for studying immunotoxicity also provides important insights preventing hazards health.

Language: Английский

Citations

0