Iron and Ammonia: A Deadly Combination for Head-Kidney Macrophages of Yellow Catfish (Pelteobagrus Fulvidraco) - Uncovering the Intensified Ferroptosis, Oxidative Stress, and Inflammatory Reactions DOI

Kewei He,

Xinran Long,

Haibo Jiang

et al.

Published: Jan. 1, 2023

Abstract:For fish, ammonia toxicity is closely related to ferroptosis, oxidative stress and inflammatory responses. Iron an essential trace element that plays a key role in many biological processes for cells organisms, including response inflammation. The current study aimed investigate the effect of iron on above three aspects indicators upon exposure fish. head kidney macrophages yellow catfish were randomized into CON group (normal control), AM (0.046 mg L-1 total nitrogen), Fe (20 µg mL-1 FeSO4), Fe+AM FeSO4, 0.046 nitrogen). Cells pretreated with FeSO4 6 h followed by 24 h. results showed supplementation triggered ferroptosis (an increase level, upregulation transferrin receptor 1) (ROS accumulation, reduction T-AOC, downregulation SOD, CAT Nrf2), up-regulated mRNA expression p53 anti-inflammatory M2 macrophage maker (Arg-1), promoted progression small portion from early apoptosis late apoptotic cells. But combination treatment caused stronger intracellular reaction than either alone. Meanwhile, there was obvious necrotic groups. findings this indicate functions fish may vary inconsistently presence or absence stress.

Language: Английский

The SLC38A9–mTOR axis is involved in autophagy in the juvenile yellow catfish (Pelteobagrus fulvidraco) under ammonia stress DOI
Xue Li, Shidong Wang, Muzi Zhang

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 343, P. 123211 - 123211

Published: Dec. 22, 2023

Language: Английский

Citations

5
Dissolved oxygen and ammonia affect ammonia production via GDH/AMPK signaling pathway and alter flesh quality in Chinese perch (Siniperca chuatsi) DOI

Ru Yang,

Xuange Liu,

Yong Liu

et al.

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 50(3), P. 1237 - 1249

Published: March 22, 2024

Language: Английский

Citations

1
New insights into oxidative damage to yellow catfish (Pelteobagrus fulvidraco) muscle by acute ammonia stress DOI
Yanjun Tang, Muzi Zhang, Ming Li

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 596, P. 741850 - 741850

Published: Nov. 2, 2024

Language: Английский

Citations

1
Moderate Dietary Saccharicterpenin Supplement Ameliorates Growth Performance, Muscle Antioxidant Capacity and Quality of the Crayfish (Procambarus Clarkii) DOI

changdong hu,

Wenping Yang, Hongyan Tian

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Acute Ammonia Exposure Induces Biochemical, Gene Expression, and Histopathological Alterations in Topmouth Culter (Culter Alburnus) from Xingkai Lake DOI

Shuyan Bai,

Banghua Xia,

Junfei Yu

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Antioxidant and Metabolic Response to Acute Acidification Stress of Juvenile Yellowfin Tuna (Thunnus albacares) DOI Creative Commons

Xiaoyan Wang,

Rui Yang,

Zhengyi Fu

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 970 - 970

Published: June 8, 2024

This study aimed to explore the impact of acute acidification on antioxidant, metabolic performance, and liver histology juvenile yellowfin tuna. The experiment subjected tuna a pH gradient environment 8.1, 7.6, 7.1, 6.6 for 48 h. findings indicate that seawater 7.1 significantly impacts antioxidant systems in comparison control group. At there were observed increases glutathione reductase (GR), total capacity (T-AOC), lactate dehydrogenase (LDH), hexokinase (HK), pyruvate kinase (PK), sodium-potassium ATPase (Na+K+-ATP), calcium-magnesium (Ca2+Mg2+-ATP). Conversely, low-density lipoprotein cholesterol (LDL-C), high-density (HDL-C), triglycerides (TGs) not different across treatment groups. However, an increase transaminases at suggested potential damage, which was further supported by structural tissue degeneration hepatocyte vacuolation. In conclusion, under conditions stress, is decrease suppression levels tuna, leading oxidative damage. lays foundation in-depth understanding response mechanisms as well healthy farming broader context acidification.

Language: Английский

Citations

0
Exploring a novel toxicological mechanism: Ammonia-N induces neurotoxicity, oxidative damage in brain tissue, and modulates the expression of central appetite factors to suppress feeding in juvenile Siberian sturgeon (Acipenser baerii Brandt) DOI
Xin Zhang, Q. Hu, Huimin Sun

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741725 - 741725

Published: Oct. 6, 2024

Language: Английский

Citations

0
Effects of Ammonia Stress on Liver Tissue Structure, Enzyme Activities, and Metabolome of Juvenile Largemouth Bass Micropterus salmoides DOI Creative Commons

Decheng Pu,

Zhengxi Wang,

Jishu Zheng

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 649 - 649

Published: Nov. 21, 2024

Background: Ammonia, a ubiquitous contaminant in aquatic ecosystems, poses multifaceted threats to fish species at elevated concentrations. Methods: In order investigate the toxic effects of chronic ammonia stress on liver juvenile Micropterus salmoides, present experiment was conducted differences changes tissue structure, enzyme activities, and metabolomes after 28 days exposure (0, 4, 8, 16 mg/L). Results: The findings revealed that induced significant oxidative liver, manifesting decreased activities antioxidant enzymes SOD GSH-Px, levels GSH, GST, MDA, heightened immune LZM, ALP, ACP. An increase concentration exacerbated damage. Metabolome analysis further unveiled perturbations metabolites salmoides exposed ammonia, with Ala-His emerging as potentially pivotal functional substance under stress. Specifically, 4 mg/L group responded toxicity by augmenting GSH L-Carnosine levels, 8 detoxified via upregulation L-Glutamine, mitigated through urea synthesis pathway. Conclusions: This research offers preliminary insights into toxicological responses It is suggested duration exceeding high-density aquaculture should not exceed 7 days.

Language: Английский

Citations

0
Iron and Ammonia: A Deadly Combination for Head-Kidney Macrophages of Yellow Catfish (Pelteobagrus Fulvidraco) - Uncovering the Intensified Ferroptosis, Oxidative Stress, and Inflammatory Reactions DOI

Kewei He,

Xinran Long,

Haibo Jiang

et al.

Published: Jan. 1, 2023

Abstract:For fish, ammonia toxicity is closely related to ferroptosis, oxidative stress and inflammatory responses. Iron an essential trace element that plays a key role in many biological processes for cells organisms, including response inflammation. The current study aimed investigate the effect of iron on above three aspects indicators upon exposure fish. head kidney macrophages yellow catfish were randomized into CON group (normal control), AM (0.046 mg L-1 total nitrogen), Fe (20 µg mL-1 FeSO4), Fe+AM FeSO4, 0.046 nitrogen). Cells pretreated with FeSO4 6 h followed by 24 h. results showed supplementation triggered ferroptosis (an increase level, upregulation transferrin receptor 1) (ROS accumulation, reduction T-AOC, downregulation SOD, CAT Nrf2), up-regulated mRNA expression p53 anti-inflammatory M2 macrophage maker (Arg-1), promoted progression small portion from early apoptosis late apoptotic cells. But combination treatment caused stronger intracellular reaction than either alone. Meanwhile, there was obvious necrotic groups. findings this indicate functions fish may vary inconsistently presence or absence stress.

Language: Английский

Citations

0