Investigating the potential immunomodulatory effects of commercial oral probiotic supplements on equine gastrointestinal tract barrier function

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 21, 2025

Background Oral probiotic dietary supplements are widely used in veterinary medicine, including horses. It is hypothesized that the presence of strains can both modulate intestinal microbiota and affect mucosal immunity parameters. Such a study has not yet been conducted Methods This involved 12 healthy horses, which were randomly divided into control group received commercial oral formula containing Lactobacillus rhamnosus , Pedioccus acidilactici or Enterococcus faecium for 84 days. Fecal samples collected from all horses on day 0 (D0), 28 days after starting (D28), 56 (D56), (D84) stopping (DX) treatment. The subjected to microbiome analysis via next-generation sequencing hypervariable regions V3-V4 V7-V9 16S rRNA gene short-chain fatty acids HPLC fecal secretory immunoglobulin A (SIgA) quantification ELISA. Results Microbiome revealed no significant differences either alpha beta diversity parameters between groups. No detected samples. Significant changes three taxa: family Bacteroidales RF16 group, genus Erysipelotrichaceae UCG-004, Fibrobacter during In cases, there was gradual decrease relative abundance over time. concentrations SCFAs, specifically acetic propionic acids, significantly increased time groups according generalized linear mixed effects (GLME) model. There SIgA secretion. Conclusion present effect use supplement composition microbiota.

Language: Английский

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Exploring the Effect of Gastrointestinal Prevotella on Growth Performance Traits in Livestock Animals

Animals, Journal Year: 2024, Volume and Issue: 14(13), P. 1965 - 1965

Published: July 2, 2024

Microorganisms in the rumen play a crucial role determining most efficient utilization rate of nutrients. Among these microorganisms,

Language: Английский

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Gut Microbiota of Ruminants and Monogastric Livestock: An Overview

Animals, Journal Year: 2025, Volume and Issue: 15(5), P. 758 - 758

Published: March 6, 2025

The diversity and composition of the gut microbiota are widely recognized as fundamental factors influencing well-being productivity domestic animals. Advancements in sequencing technologies have revolutionized studies this research field, allowing for deeper insights into functionality livestock. Ruminants monogastric animals exhibit distinct digestive systems characteristics: ruminants rely on fermentation, while monogastrics use enzymatic digestion, simpler stomach structures, except horses rabbits, where both processes coexist. Understanding microbiota's impact animal types is essential optimizing production efficiency promoting health. Following perspective, present manuscript review aims to provide a comprehensive overview (such cattle, sheep, goats) (including horses, pigs, chickens).

Language: Английский

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Effect of Management System on Fecal Microbiota in Arabian Horses: Preliminary Results

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 309 - 309

Published: March 28, 2025

The gut microbiota plays a basic role in maintaining the psychophysical health and well-being of horses. Understanding complex interactions among microbial communities relation to age, management, is topic growing interest. equine microbiota, given its complexity, subject variations caused by internal or external stressors that can lead metabolic problems pathologies, i.e., obesity laminitis. aim this study was characterize fecal 12 purebred Arabian horses compare two distinct management systems (Group 1 = box 22 h/day + paddock 2 Group 24 h). Fecal samples were analyzed using high-throughput sequencing 16S rRNA V3-V4 amplicons. predominantly composed Firmicutes (32–53%) Bacteroidetes (32–47.8%), with Ruminococcaceae emerging as most prevalent bacterial family (10.9–24%). Comparisons alpha beta diversity revealed no statistically significant differences between groups based on different systems. This preliminary characterization contributes valuable data for understanding lays groundwork future studies relationship intestinal health, performance, strategies.

Language: Английский

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Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls

Journal of Veterinary Internal Medicine, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 1, 2025

Abstract Background Altered gut microbiota has been associated with dopaminergic degenerative diseases in people, but studies on horses pituitary pars intermedia dysfunction (PPID) are lacking. Hypothesis/Objectives Investigate the effect of PPID fecal horses. Animals Nine and 13 age‐matched control Methods Prospective study. Fecal samples were collected bimonthly. Microbial analysis used 16S rRNA sequencing to determine relative abundance at genus phylum levels, assess alpha beta diversity identify core microbiota. Results Horses had decreased abundances Christensenellaceae R‐7 group (median; 95% confidence interval [CI]: PPID, 2.04; 1.82‐2.35 vs control, 2.54; 2.37‐2.76; P = .02) NK4A214 (PPID, 2.21; 2.02‐2.56 2.62; 2.44‐2.85; .05), significant lower Romboutsia (log2FoldChange −3.54; .04) Peptococcaceae uncultured −0.89; by differential analysis. However, Fibrobacter 0.74; was significantly higher group. A observed ( .004), whereas varied months .001). Seven unique genera identified 12 Conclusions Clinical Importance The microbial composition is altered PPID. These findings support potential role microbiota‐gut‐brain axis pathogenesis

Language: Английский

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Changes in bacterial viability after preparation and storage of fecal microbiota transplantation solution using equine feces

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18860 - e18860

Published: Feb. 20, 2025

Fecal microbiota transplantation (FMT) has been used as a treatment option for horses (Equus caballus) with gastrointestinal diseases. Several preparation and conservation protocols to improve bacterial survival have studied in other species. This study aimed evaluate the impact of oxygen exposure different protectant solutions on viability before after freezing using horse feces. samples from 10 healthy were aliquoted diluted cryoprotectant containing antioxidants (n = 40) or 10% glycerol 40). Half aliquots each dilution condition prepared inside an anaerobic chamber, while half under ambient air conditions. Each sample was also analyzed fresh at -20 °C 90 days. Bacterial assessed flow cytometry. A mixed linear model Friedman Wilcoxon tests depending data distribution. Freeze-thawing decreased by 47% (mean ± SD: 51 27% before, 27 8% after; p < 0.001). Glycerol superior (32 glycerol, 24 cryoprotectant; Oxygen did not affect (p 0.13). There no statistical difference between 0.16). Fresh FMT may be better treating dysbiosis, but if cannot avoided, should dilute

Language: Английский

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Nutrition as an effective preventative medicine

UK-Vet Equine, Journal Year: 2025, Volume and Issue: 9(2), P. 58 - 65

Published: March 2, 2025

Nutrition plays a pivotal role in maintaining optimal health, influencing physical wellbeing, performance and behaviour. Horse owners frequently rely on veterinarians for expert advice nutrition, but many report lack of confidence their nutritional expertise. Poor dietary practices can lead to significant health issues. This review examines the nutrition as an effective preventative medicine, addressing challenges face equine guidance growing body research highlighting importance good its link disease. Additionally, explores advancements understanding gut microbiome, including impact various interventions such prebiotics, probiotics postbiotics. With rising interest these offer promising strategies enhance disease resistance horses. Finally, discusses practical approaches optimising potential supplements, while emphasising evidence-based veterinary nutrition.

Language: Английский

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Preliminary Functional Analysis of the Gut Microbiome in Colic Horses

Animals, Journal Year: 2024, Volume and Issue: 14(22), P. 3222 - 3222

Published: Nov. 10, 2024

The gut microbiome plays a critical role in maintaining horse health, influencing digestion, immunity, and overall well-being. However, certain conditions like colic, there is evidence of significant alterations the microbial community. To analyze composition fecal enriched predicted metabolic functions horses with colic compared to control group, 14 were recruited. From stool sample, DNA extraction was carried out for subsequent 16S rRNA metagenomic analysis. analyzed from sequences each sample using QIIME version 1.8.0 DADA2 1.22 programs. PICRUSt2 used predict functions. Statistical analyses performed Mann-Whitney U test Python scipy v1 package. microbiomes both groups dominated by

Language: Английский

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Synergistic effects of RISCO–NUTRIFOUR probiotic mixtures as feed additives on growth performance and carcase characteristics of broiler chickens over 28 days

Italian Journal of Animal Science, Journal Year: 2024, Volume and Issue: 24(1), P. 33 - 42

Published: Dec. 19, 2024

Antibiotic substitutes in the feed can have a positive effect on performance and carcase characteristics lead to healthier chicks with higher body weight. To investigate effects of feed-based probiotic RISCO–NUTRIFOUR® solution (RNFS) at different doses parameters attributes broilers. A total 288 Ross 308–day-old birds were treated basal diet six dietary treatment levels (T1–T3 = RNFS level 1, 2, or 3) (0.4%, 0.2%, 0.1% RNFS/ton feed), T4= Bacillus subtilis 'CLOSTAT®', T5= Saccharomyces cerevisiae (Baker's yeast), T6= without addition (negative control). Performance indicators estimated during 0–14, 14–28, 0–28. On day 28, absolute relative weights evaluated. Broilers fed diets, particularly 3, heavier 28 days, acquired more weight, converted efficiently meat, degree than CLOSTAT Baker's yeast groups. Throughout study, treatments had no exception breast leg weights. In conclusion, feeding probiotics RNFS, especially low (0.1% "1 L/ton"), was able maximise broiler while maintaining characteristics.

Language: Английский

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Nutrient utilization and energy balance profile in probiotic supplemented Asian elephants

Eurasian journal of veterinary sciences, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Aim: A study was methodized to evaluate whether supplementation of probiotics could impact the utilization nutrients and energy in Asian elephants. Materials Methods: Eighteen elephants were randomly selected for 60 days experiment including five digestibility trial divided into three groups with six each i.e. control no probiotic (T1) other two Lactobacillus acidophilus T2 Saccharomyces cerevisiae T3 at 1 × 109 CFU/gm concentrate per 50 kg body weight/ day orally. Results: : The intakes ether extract total ash revealed significant whereas coefficients acid-insoluble exhibited a highly effect treatment. However, values proximate principles fibre fractions not reveal any effect. Practical nutritional worth, plane nutrition balance profile also found be non among groups. Increasing trend intake energy; relative difference associated increased DM NDF digestibility. mean TDN%, NR, digestible OM TDN obtained higher supplemented than group. Conclusion: It is concluded that exhibit on further research needed examine effects high doses, strain viability probiotics.

Language: Английский

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