Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: Nov. 13, 2024

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: March 25, 2025

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0
Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: April 11, 2025

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0
Molecular insights into heterosis for Vibrio alginolyticus resistance in crosses between two geographically distinct wild populations of Ruditapes philippinarum DOI
Hongda Li, Hongtao Nie, Bin Wang

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102831 - 102831

Published: April 21, 2025

Language: Английский

Citations

0
Pathological and miRNA-mRNA Analyses Provide New Insights into the Immune Response of Clams to Vibrio Infection DOI
Jianxin Jia, Hongtao Nie

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(3)

Published: April 23, 2025

Language: Английский

Citations

0
Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: Nov. 13, 2024

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0