Free Gossypol Removal and Nutritional Value Enhancement of Cottonseed Meal via Solid-State Fermentation with Rhodotorula mucilaginosa TG529 DOI Creative Commons

Bifan Liu,

Huanyu Liu,

Dianji Liu

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1463 - 1463

Published: Aug. 27, 2024

The presence of free gossypol (FG) in cottonseed meal (CSM) greatly limits the use CSM as a high-quality protein feed. Microbial fermentation is an effective method to simultaneously reduce FG and improve nutritional value CSM. In this study, using potato dextrose agar containing acetic selective medium humus soil from cotton fields source, we isolated six strains fungi capable tolerating FG. With inoculation ratio 8% (8 mL × 106 CFU/mL cells or spores 100 g fermented CSM), 50% moisture content, temperature 30 °C, was for 5 days. results showed that strain F had highest removal rate at 56.43%, which identified Rhodotorula mucilaginosa (R. mucilaginosa) named R. TG529. Further optimization revealed when time extended 11 days, TG529 achieved maximum 73.29%. Compared original sample, treatment with significantly increased contents crude protein, acid-soluble 18 amino acids, while reducing fiber, neutral detergent fiber (NDF), acid (FCSM). Using atmospheric room plasma mutagenesis TG529, it found mutated phenylalanine FCSM, reduced NDF enhanced 76.50%. summary, study screened mutagenized detoxifying fungus, mucilagnosa can effectively content by solid-state fermentation.

Language: Английский

Effect of biotin on growth performance, serum biochemical profiles, and histomorphological changes in acetate gossypol-treated mice DOI Creative Commons

Qiyu Luo,

Tieshan Xu,

Zulibina Ainiwaer

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: April 15, 2025

The objective of this study was to determine the effects biotin on growth performance, serum biochemical profiles, and morphology acetate gossypol-treated mice. Seventy-five healthy male Kunming mice were randomly assigned five treatment groups: control group (C), (BC), gossypol (AGC), prevention (BP) (BT). examined visceral index, profiles histomorphological changes results showed that compared with AGC mice, average daily feed intake BP BT increased by 40.47 45.69% ( p < 0.05). Biotin reduced elevated blood urea nitrogen (BUN) levels in aspartate aminotransferase (AST), total protein (TP) BUN increase values villus height/crypt depth duodenum jejunum In conclusion, potentially performance it also has a positive effect

Language: Английский

Citations

0
Free Gossypol Removal and Nutritional Value Enhancement of Cottonseed Meal via Solid-State Fermentation with Rhodotorula mucilaginosa TG529 DOI Creative Commons

Bifan Liu,

Huanyu Liu,

Dianji Liu

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1463 - 1463

Published: Aug. 27, 2024

The presence of free gossypol (FG) in cottonseed meal (CSM) greatly limits the use CSM as a high-quality protein feed. Microbial fermentation is an effective method to simultaneously reduce FG and improve nutritional value CSM. In this study, using potato dextrose agar containing acetic selective medium humus soil from cotton fields source, we isolated six strains fungi capable tolerating FG. With inoculation ratio 8% (8 mL × 106 CFU/mL cells or spores 100 g fermented CSM), 50% moisture content, temperature 30 °C, was for 5 days. results showed that strain F had highest removal rate at 56.43%, which identified Rhodotorula mucilaginosa (R. mucilaginosa) named R. TG529. Further optimization revealed when time extended 11 days, TG529 achieved maximum 73.29%. Compared original sample, treatment with significantly increased contents crude protein, acid-soluble 18 amino acids, while reducing fiber, neutral detergent fiber (NDF), acid (FCSM). Using atmospheric room plasma mutagenesis TG529, it found mutated phenylalanine FCSM, reduced NDF enhanced 76.50%. summary, study screened mutagenized detoxifying fungus, mucilagnosa can effectively content by solid-state fermentation.

Language: Английский

Citations

3