Compensatory mechanisms in γδ T cell-deficient chickens following Salmonella infection DOI Creative Commons
Felix Tetzlaff, Ulrich Methner, Theresa von Heyl

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 14, 2025

Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene transcription sorted (TCR1 + ) TCR1 - subsets as well tissue was measured by RT-qPCR. Strikingly, lacking had significantly higher bacterial liver more extensive invasion during early stages compared birds. In infected displayed increased percentage CD25 NK-like cells. both blood tissue, demonstrated an absolute number CD8αα +hi (CD4 ). Conversely, exhibited augmented count CD4 population after infection, which might include αβ At 7 days post (dpi), expression analysis revealed elevated activation marker IL-2Rα proinflammatory cytokines (IL-17A, IFN-γ) from By 12 dpi, these differences diminished levels animals. Our findings demonstrate that protection Enteritidis later functions appear be replaced other

Language: Английский

Compensatory mechanisms in γδ T cell-deficient chickens following Salmonella infection DOI Creative Commons
Felix Tetzlaff, Ulrich Methner, Theresa von Heyl

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 14, 2025

Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene transcription sorted (TCR1 + ) TCR1 - subsets as well tissue was measured by RT-qPCR. Strikingly, lacking had significantly higher bacterial liver more extensive invasion during early stages compared birds. In infected displayed increased percentage CD25 NK-like cells. both blood tissue, demonstrated an absolute number CD8αα +hi (CD4 ). Conversely, exhibited augmented count CD4 population after infection, which might include αβ At 7 days post (dpi), expression analysis revealed elevated activation marker IL-2Rα proinflammatory cytokines (IL-17A, IFN-γ) from By 12 dpi, these differences diminished levels animals. Our findings demonstrate that protection Enteritidis later functions appear be replaced other

Language: Английский

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