Optimization of In Vitro Maturation in Ovine Oocytes: A Comparative Study of Maturation Media DOI Open Access

Abbas Darzi Nia,

Mohammad Zandi, Annahita Ghaedrahmati

et al.

Gene Cell and Tissue, Journal Year: 2025, Volume and Issue: 12(2)

Published: March 28, 2025

Background: One of the most common techniques in assisted reproductive technologies (ART) is vitro embryo production (IVEP), success which depends on intrinsic quality oocytes and composition culture medium. Objectives: This study investigates effects gonadotropin hormones (such as eCG hCG) along with antioxidant quercetin maturation ovine oocytes, well impact different media expression Bcl2 Bax genes blastocysts. Methods: After washing cumulus-oocyte complexes (COCs) that exhibited three or more layers cells homogeneous cytoplasm were selected matured BO-IVM, TCM (TCM-199 + 10% FBS follicular fluid 5 mg/mL FSH 1 estradiol-17β 0.81 mM sodium pyruvate 50 gentamicin sulfate), TCM+ (TCM 20 µg/mL hCG 15 quercetin) for 24 hours at 38.5°C, 20% O2, 5% CO2. maturation, fertilized frozen ram semen, presumed zygotes cultured BO-IVC under uniform conditions. Embryo development occurred 38.5°C a humid atmosphere CO2, 90% N2. Results: The results indicated BO-IVM medium had significantly higher mean percentage zygotes, morulae, blastocysts, hatched blastocysts compared to (P < 0.05). Moreover, blastocyst formation frequent than However, demonstrated significant improvement other media. Gene analysis showed no difference among groups, while was Overall, identified best option optimizing oocyte producing high-quality ovine, yielding better without hormonal compounds. Conclusions: this addition growth factors improved performance further research needed enhance commercial

Language: Английский

Optimization of In Vitro Maturation in Ovine Oocytes: A Comparative Study of Maturation Media DOI Open Access

Abbas Darzi Nia,

Mohammad Zandi, Annahita Ghaedrahmati

et al.

Gene Cell and Tissue, Journal Year: 2025, Volume and Issue: 12(2)

Published: March 28, 2025

Background: One of the most common techniques in assisted reproductive technologies (ART) is vitro embryo production (IVEP), success which depends on intrinsic quality oocytes and composition culture medium. Objectives: This study investigates effects gonadotropin hormones (such as eCG hCG) along with antioxidant quercetin maturation ovine oocytes, well impact different media expression Bcl2 Bax genes blastocysts. Methods: After washing cumulus-oocyte complexes (COCs) that exhibited three or more layers cells homogeneous cytoplasm were selected matured BO-IVM, TCM (TCM-199 + 10% FBS follicular fluid 5 mg/mL FSH 1 estradiol-17β 0.81 mM sodium pyruvate 50 gentamicin sulfate), TCM+ (TCM 20 µg/mL hCG 15 quercetin) for 24 hours at 38.5°C, 20% O2, 5% CO2. maturation, fertilized frozen ram semen, presumed zygotes cultured BO-IVC under uniform conditions. Embryo development occurred 38.5°C a humid atmosphere CO2, 90% N2. Results: The results indicated BO-IVM medium had significantly higher mean percentage zygotes, morulae, blastocysts, hatched blastocysts compared to (P < 0.05). Moreover, blastocyst formation frequent than However, demonstrated significant improvement other media. Gene analysis showed no difference among groups, while was Overall, identified best option optimizing oocyte producing high-quality ovine, yielding better without hormonal compounds. Conclusions: this addition growth factors improved performance further research needed enhance commercial

Language: Английский

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