Inhibition of some multidrug-resistant bacteria using prepared essential oil Nanoemulsion formulas and their mode of action DOI Creative Commons

Reda H Amasha

Journal of Contemporary Medical Sciences, Journal Year: 2024, Volume and Issue: 10(2)

Published: April 30, 2024

Objective: The use of essential oils for the preparation different nanoemulsions (NEa, NEb and NEc) detect their biological activities. Methods: Nanoemulsiona were prepared by mixing oil (10 %) Coriandrum seed sativum, Ginger roots Zingiber officinale, or Achillea leaves clavennae), sterile water, surfactants (Brij 30, Span 20, Triton X-100, Tween 60) in addition to 0.1M Tris-HCl buffer (pH 7.22). three examined characterized. Their antibacterial activities against some multidrug-resistant Gram-positive negative bacteria. Results: maximum activity was recorded By Serratia marcescens, Staphylococcus aurous, Micrococcus luteus, Enterococcus faecalis, Streptococcus pyogenes, saprophyticus while lower obtained Klebsiella pneumonia Escherichia coli. minimum inhibitory concentrations (MICs) ranged from 40-60 µl/ml nutrient broth. affects cell counts morphology S. marcescens destroys bacterial cells reducing respiration enhancing permeability leakage protein, DNA, potassium membrane. no toxicity Artimia salina HaCaT line. It decreases biofilm formation 51%, infection, attachment. Conclusion: This study suggests that NEs have great human pathogens process modes action, thus they can be used effectively treat infections.

Language: Английский

Unveiling the Wonders of Bacteria-Derived Extracellular Vesicles: From Fundamental Functions to Beneficial Applications DOI Creative Commons

Mariam Rima,

Mariam Dakramanji,

E. Hayek

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42509 - e42509

Published: Feb. 1, 2025

:Extracellular vesicles (EVs), are critical mediators of intercellular communication and exhibit significant potential across various biomedical domains. These nano-sized, membrane-encapsulated entities have captured substantial interest due to their diverse roles in pathogenesis promising therapeutic applications. EVs manage numerous physiological processes by transferring bioactive molecules, including proteins, lipids, nucleic acids, between cells. This review delves into the factors influencing properties EVs, such as temperature stress conditions, which collectively influence size, composition, functional attributes. We also describe emerging emphasizing involvement microbial interactions, immune modulation, antimicrobial resistance spread innovative diagnostic instruments. Despite applications, advancement EV-based therapies faces several challenges, will be discussed. By elucidating these elements, we aim provide a comprehensive overview transformative revolutionizing diagnostics therapeutics medicine.

Language: Английский

Citations

4
Evaluation of Potential Probiotic Properties and In Vivo Safety of Lactic Acid Bacteria and Yeast Strains Isolated from Traditional Home-Made Kefir DOI Creative Commons
Angela Maione,

Marianna Imparato,

Annalisa Buonanno

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(7), P. 1013 - 1013

Published: March 26, 2024

Probiotics are known for their health-promoting resources and considered as beneficial microorganisms. The current study focuses on the isolation, a complete in vitro vivo characterization, of yeast lactic acid bacteria acquired from traditional homemade kefir order to assess potentiality probiotic candidates. In particular, isolates Pichia kudriavzevii Y1, Lactococcus lactis subsp. hordniae LAB1 LAB2 were subjected characterization evaluate suitability probiotics. Resistance bile salts, auto-aggregation, co-aggregation, hydrophobicity, biofilm production capability examined, well antioxidant activity. A safety assessment was also conducted confirm non-pathogenic nature isolates, with hemolysis assay antibiotic resistance assessment. Moreover, mortality invertebrate model Galleria mellonella evaluated. Current findings showed that P. exhibited estimable properties, placing them promising candidates functional foods. Both isolated this work could be classified potential probiotics advantageous traits, including antimicrobial activity against enteric pathogens good adhesion ability intestinal cells. This revealed origin different microorganisms may enhance health wellness.

Language: Английский

Citations

7
Investigation on Klebsiella pneumoniae in the field of extracellular vesicles DOI Creative Commons
Jun Wu, Yuheng Liu, Jin Su

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Klebsiella pneumoniae (KP), recognized for its pronounced antibiotic resistance, is a prevalent agent of nosocomial infections such as hospital‐acquired pneumonia. The alarming rate serious and associated mortality renders KP significant public health threat. Extracellular vesicles (EVs) are pivotal in KP's physiological pathological mechanisms, facilitating material information transfer mediating interactions between the bacteria host. critical role EVs enhances our understanding pathophysiology, disease progression, strategies infection control. This review emphasizes mechanics resistance instrumental bacterium‐host interplay, proposing promising research focus advancing diagnosis, therapy, prevention.

Language: Английский

Citations

0
Inhibition of some multidrug-resistant bacteria using prepared essential oil Nanoemulsion formulas and their mode of action DOI Creative Commons

Reda H Amasha

Journal of Contemporary Medical Sciences, Journal Year: 2024, Volume and Issue: 10(2)

Published: April 30, 2024

Objective: The use of essential oils for the preparation different nanoemulsions (NEa, NEb and NEc) detect their biological activities. Methods: Nanoemulsiona were prepared by mixing oil (10 %) Coriandrum seed sativum, Ginger roots Zingiber officinale, or Achillea leaves clavennae), sterile water, surfactants (Brij 30, Span 20, Triton X-100, Tween 60) in addition to 0.1M Tris-HCl buffer (pH 7.22). three examined characterized. Their antibacterial activities against some multidrug-resistant Gram-positive negative bacteria. Results: maximum activity was recorded By Serratia marcescens, Staphylococcus aurous, Micrococcus luteus, Enterococcus faecalis, Streptococcus pyogenes, saprophyticus while lower obtained Klebsiella pneumonia Escherichia coli. minimum inhibitory concentrations (MICs) ranged from 40-60 µl/ml nutrient broth. affects cell counts morphology S. marcescens destroys bacterial cells reducing respiration enhancing permeability leakage protein, DNA, potassium membrane. no toxicity Artimia salina HaCaT line. It decreases biofilm formation 51%, infection, attachment. Conclusion: This study suggests that NEs have great human pathogens process modes action, thus they can be used effectively treat infections.

Language: Английский

Citations

2