Molecular Biology, Journal Year: 2024, Volume and Issue: 58(6), P. 980 - 1002
Published: Dec. 1, 2024
Language: Английский
Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 181 - 181
Published: Jan. 16, 2025
Using a murine osteomyelitis model, we recently demonstrated that Staphylococcus aureus sarA and sarA/agr mutants generated in the USA300 strain LAC are attenuated to greater extent than an isogenic agr mutant this can be attributed significant increased production of extracellular proteases both mutants. Based on this, used mass-based proteomics approach compare proteomes LAC, its agr, sarA, mutants, derivatives all four these strains unable produce aureolysin, SspA, SspB, ScpA, or SplA-F. This allowed us identify proteins were present reduced amounts owing proteases. A total 1039 detected conditioned media (CM) from overnight cultures protease-mediated degradation was shown contribute abundance 224 (21.6%) CM Among specific previously implicated pathogenesis therapeutic recalcitrance S. osteomyelitis. demonstrates ability limit protease plays key role post-translational remodeling proteome degree correlated with virulence our it does so irrespective functional status agr. also suggests at least some may viable targets for prophylactic intervention.
Language: Английский
Citations
1
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 6589 - 6602
Published: July 1, 2024
Abstract: Orthopedic infection is one of the most intractable orthopedic problems. Bacteria resistant to antibiotics also develop gradually. Chitosan widely used in Biomedical field because its high biocompatibility, biodegradability, and antibacterial activity. Chitosan-based drug delivery systems are frequently utilized produce controlled medication release. When combined with antibiotics, synergistic effects can be achieved. nanoparticles applications systems. The focus this review provide information on new methods being developed for chitosan-based bone treatment, including chitosan purposes, Ch-loaded metal, as immune adjuvants. It may Provide ideas fundamental research prospects future clinical infections. Keywords: nanoparticles, infection, staphylococcus aureus
Language: Английский
Citations
6
ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: March 14, 2025
Infected bone defects, caused by bacterial contamination following disease or injury, result in the partial loss destruction of tissue. Traditional transplantation and other clinical approaches often fail to address therapeutic complexities these conditions effectively. In recent years, advanced biomaterials have attracted significant attention for their potential enhance treatment outcomes. This review explores pathogenic mechanisms underlying infected including biofilm formation internalization into cells, which allow bacteria evade host immune system. To control infection facilitate repair, we focus on antibacterial materials regeneration. A detailed introduction is given intrinsically (e.g., metal alloys, oxide materials, carbon-based hydroxyapatite, chitosan, Sericin). The functionality repair can be enhanced through strategies such as incorporation antimicrobial ions, surface modification, combined use multiple treat defects. Key innovations discussed include that release agents, functional contact biomaterials, bioresponsive collectively efficacy. Research translation has also facilitated practical application prevention healing. conclusion, advancements provide promising pathways developing more biocompatible, effective, personalized therapies reconstruct
Language: Английский
Citations
0
Cureus, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 1, 2024
Biofilms are intricate microbial communities on various surfaces, including medical devices and biological tissues, encased within a protective matrix of extracellular polymeric substances. Their formation persistence significant factors in the pathogenesis chronic infections, contributing to complexity treatment increased resistance antimicrobial agents. This review explores multifaceted nature biofilms, focusing their formation, structure, genetic environmental that contribute resilience. particularly problematic such as those associated with implants persistent wounds, due ability evade both host immune response conventional therapeutic strategies. The also discusses current challenges diagnosing biofilm-associated infections limitations existing options. Emerging approaches, novel antibiofilm agents, physical disruption techniques, therapies phage therapy, examined for potential improve outcomes. Innovations drug delivery systems preventive measures, biofilm-resistant materials, highlighted promising developments. comprehensive overview aims provide insights into mechanisms biofilm-related guide future research clinical practice. contributes ongoing efforts enhance patient care combat growing challenge by addressing critical need effective strategies manage prevent infections.
Language: Английский
Citations
3
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: July 25, 2024
Hypervirulent
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13241 - 13241
Published: Dec. 10, 2024
In orthopedics, the use of anti-infective biomaterials is considered most promising strategy to contrast bacterial contamination implant surfaces and reduce infection rate. KSL, KSL-W, Dadapin-1 are three antimicrobial peptides (AMPs) that possess significant antibacterial properties, making them candidates for producing not based on antibiotics. To fully assess their true potential, this study explores in detail cytocompatibility human osteoblast-like MG63 cells, murine fibroblastoid L929 hMSCs. end, cytotoxicity AMPs terms IC50 was tested over a range concentrations 450–0.22 µg/mL using ATP bioluminescence assay. The tests were performed both presence absence bovine serum effects components peptide stability. values obtained under stringent conditions used extrapolate selectivity index (S.I.) toward salient species. medium containing serum, all exhibited minimal no cytotoxicity, with exceeding 100 µg/mL. lowest KSL-W highest stability, KSL selectivity. Overall, these findings highlight potential future production materials.
Language: Английский
Citations
1
Journal of Multidisciplinary Healthcare, Journal Year: 2024, Volume and Issue: Volume 17, P. 3057 - 3069
Published: July 1, 2024
Objective: Bibliometric analysis is commonly used to visualize the knowledge foundation, trends, and patterns in a specific scientific field by performing quantitative evaluation of relevant literature. The purpose this study was perform bibliometric recent studies orthopedic biofilm research identify its current trends hotspots. Methods: Research were retrieved from Web Science Core Collection Scopus databases analyzed bibliometrix with R package (4.2.2). Results: A total 2426 literature included study. Journal orthopaedic Clinical orthopaedics related ranked first terms productivity impact, 57 published articles 32 h -index, respectively. Trampuz A, Ohio State Univ United States as most productive authors, institutions, countries. Biofilm formation, role sonication, biomaterial mechanism antibiotic loading have been investigated trend hotspots research. Conclusion: This provides thorough overview state art offers valuable insights into field. Keywords: biofilm, infection, analysis, hotspot,
Language: Английский
Citations
0
Molecular Biology, Journal Year: 2024, Volume and Issue: 58(6), P. 980 - 1002
Published: Dec. 1, 2024
Language: Английский
Citations
0