High-Performance Liquid Chromatography with DAD Detection for the Determination of Cannabinoids in Commercial Veterinary CBD Oil DOI Creative Commons
Zehra Hajrulai-Musliu, Elizabeta Dimitrieska-Stojković,

Dimitar Gusheski

et al.

Pharmacy, Journal Year: 2024, Volume and Issue: 12(6), P. 181 - 181

Published: Dec. 2, 2024

The study highlights the need for quality control in evaluating medicinal plant products, especially CBD oils, before market release. Due to varying regulatory requirements, product labeling can sometimes be misleading, regarding cannabinoid concentrations such as and THC. This research focused on developing a validated high-performance liquid chromatography (HPLC) method accurately identifying quantifying key cannabinoids Commercial Veterinary Oil. main compounds identified included Cannabidivarin (CBDV), Cannabidiolic Acid (CBD-A), Cannabigerolic (CBG-A), Cannabigerol (CBG), Cannabidiol (CBD), Tetrahydrocannabivarin (THCV), Cannabinol (CBN), ∆9-Tetrahydrocannabinol (d9-THC) ∆8-Tetrahydrocannabinol (d8-THC), Cannabicyclol (CBL), Cannabichromene (CBC), Tetrahydrocannabinolic (THCA), determined line with International Conference Harmonization’s (ICH) guidelines. was linearity, accuracy, precision, limit of detection (LOD), quantitation (LOQ). It linear, correlation coefficient (R²) > 0.999. LOD LOQ values calculated from calibration curve ranged 0.05 0.13 0.50 0.61 µg/mL, respectively. also exhibited acceptable relative standard deviation lower than or equal 2%. method’s accuracy assessed through recovery percentages fell within an range 98–102 if RSD study’s rigorous methodology comprehensive findings significantly contribute analysis. protocol used analyze 14 commercial veterinary oil products Republic North Macedonia. performance parameters demonstrated that is reliable quantitatively measuring oil. analysis showed levels were consistent manufacturers’ declared specifications, no significant discrepancies labeling.

Language: Английский

High-Performance Liquid Chromatography with DAD Detection for the Determination of Cannabinoids in Commercial Veterinary CBD Oil DOI Creative Commons
Zehra Hajrulai-Musliu, Elizabeta Dimitrieska-Stojković,

Dimitar Gusheski

et al.

Pharmacy, Journal Year: 2024, Volume and Issue: 12(6), P. 181 - 181

Published: Dec. 2, 2024

The study highlights the need for quality control in evaluating medicinal plant products, especially CBD oils, before market release. Due to varying regulatory requirements, product labeling can sometimes be misleading, regarding cannabinoid concentrations such as and THC. This research focused on developing a validated high-performance liquid chromatography (HPLC) method accurately identifying quantifying key cannabinoids Commercial Veterinary Oil. main compounds identified included Cannabidivarin (CBDV), Cannabidiolic Acid (CBD-A), Cannabigerolic (CBG-A), Cannabigerol (CBG), Cannabidiol (CBD), Tetrahydrocannabivarin (THCV), Cannabinol (CBN), ∆9-Tetrahydrocannabinol (d9-THC) ∆8-Tetrahydrocannabinol (d8-THC), Cannabicyclol (CBL), Cannabichromene (CBC), Tetrahydrocannabinolic (THCA), determined line with International Conference Harmonization’s (ICH) guidelines. was linearity, accuracy, precision, limit of detection (LOD), quantitation (LOQ). It linear, correlation coefficient (R²) > 0.999. LOD LOQ values calculated from calibration curve ranged 0.05 0.13 0.50 0.61 µg/mL, respectively. also exhibited acceptable relative standard deviation lower than or equal 2%. method’s accuracy assessed through recovery percentages fell within an range 98–102 if RSD study’s rigorous methodology comprehensive findings significantly contribute analysis. protocol used analyze 14 commercial veterinary oil products Republic North Macedonia. performance parameters demonstrated that is reliable quantitatively measuring oil. analysis showed levels were consistent manufacturers’ declared specifications, no significant discrepancies labeling.

Language: Английский

Citations

0