Effects of Fucoidan on the Inhibition of Biofilm Formation of Salmonella enterica Subsp. enterica Serovar Typhimurium on Seafoods and Its Molecular Antibiofilm Mechanisms DOI Creative Commons
Anamika Roy, Pantu Kumar Roy, Sung‐Rae Cho

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 914 - 914

Published: April 16, 2025

Foodborne illnesses, particularly those caused by Salmonella enterica subsp. Serovar Typhimurium, present a significant challenge to public health, especially within the seafood industry due biofilm formation on foods. This study investigated antibiofilm potential of fucoidan, sulfated polysaccharide, against Typhimurium crab and shrimp surfaces. Fucoidan’s minimum inhibitory concentration (MIC) was determined be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, MIC) were evaluated for their impact inhibition formation. Fucoidan treatment resulted in significant, dose-dependent formation, achieving 2.61 log CFU/cm2 2.45 reductions surfaces, respectively. FE-SEM analysis confirmed disruption cell membrane damage. Real-time PCR showed downregulation quorum-sensing (luxS) virulence (rpoS, avrA, hilA) genes. These results propose that fucoidan has ability as natural antibacterial agent controlling biofilms processing, thereby enhancing food safety minimizing contamination.

Language: Английский

Effects of Fucoidan on the Inhibition of Biofilm Formation of Salmonella enterica Subsp. enterica Serovar Typhimurium on Seafoods and Its Molecular Antibiofilm Mechanisms DOI Creative Commons
Anamika Roy, Pantu Kumar Roy, Sung‐Rae Cho

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 914 - 914

Published: April 16, 2025

Foodborne illnesses, particularly those caused by Salmonella enterica subsp. Serovar Typhimurium, present a significant challenge to public health, especially within the seafood industry due biofilm formation on foods. This study investigated antibiofilm potential of fucoidan, sulfated polysaccharide, against Typhimurium crab and shrimp surfaces. Fucoidan’s minimum inhibitory concentration (MIC) was determined be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, MIC) were evaluated for their impact inhibition formation. Fucoidan treatment resulted in significant, dose-dependent formation, achieving 2.61 log CFU/cm2 2.45 reductions surfaces, respectively. FE-SEM analysis confirmed disruption cell membrane damage. Real-time PCR showed downregulation quorum-sensing (luxS) virulence (rpoS, avrA, hilA) genes. These results propose that fucoidan has ability as natural antibacterial agent controlling biofilms processing, thereby enhancing food safety minimizing contamination.

Language: Английский

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