The Incorporation of CBD into Biodegradable DL-Lactide/Glycolide Copolymers Creates a Persistent Antibacterial Environment: An In Vitro Study on Streptococcus mutans and Staphylococcus aureus

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 463 - 463

Published: April 2, 2025

Background: Cannabidiol (CBD) is a natural compound from the Cannabis sativa L. plant, which has anti-inflammatory, anti-nociceptive, neuroprotective, and antibacterial activities. Objective: The aim of this study was to develop sustained-release device CBD that can provide an effect against Gram-positive bacteria Streptococcus mutans Staphylococcus aureus for extended periods time. Methods: incorporated into biodegradable PURASORB 5010 or 7510 DL-lactide/glycolide polymers using either dimethylsulfoxide (DMSO) acetone as solvent, dried polymer scaffolds were exposed daily fresh culture bacteria. bacterial growth determined by optical density, metabolic activity biofilms MTT assay. Biofilm formation on visualized HR-SEM. Its anti-inflammatory measuring IL-6 release LPS-stimulated RAW 264.7 macrophages ELISA. Cell cytotoxicity normal Vero epithelial cells gas chromatography–mass spectrometry (GC-MS). Results: 5010/CBD had S. UA159, ATCC25923, clinical isolate multidrug-resistant (MDRSA CI-M) strain tested period up 17 days. 7510/CBD also activity, but overall, it less effective than over addition PEG400 copolymers significantly increased not 5010/CBD. sufficient reduce LPS-induced secretion macrophages, importantly, cytotoxic cells. found be between 1.12 9.43 µg/mL, far below dose 25 µg/mL. Conclusions: incorporation used prepare devices medical purposes where combined activities are desirable.

Language: Английский

Polygonatum sibiricum Extract Inactivates Cronobacter sakazakii Isolated from Powdered Infant Formula

Foodborne Pathogens and Disease, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

This study aimed to investigate the antibacterial activity and mechanism of Polygonum sibiricum extract (PSE) against Cronobacter sakazakii (C. sakazakii) isolated from powdered infant formula (PIF). The minimum inhibitory concentration (MIC), bactericidal (MBC), growth curves were measured evaluate effects. was elucidated by revealing changes in cell membrane potential, intracellular reactive oxygen species (ROS) level, permeability, protein nucleic acid leakage, morphology C. sakazakii. effects PSE biofilm on stainless steel PIF further analyzed. results showed that MIC MBC 4 mg/mL 8 mg/mL, respectively. can be completely inhibited two PSE. Cell depolarization, increased then decreased ROS content, significantly permeability (p < 0.05), more leakage acid, severely damaged found after treatment with Furthermore, reduce viable bacteria count 0.05). These findings suggest has potential serve as a natural agent contamination PIF.

Language: Английский