Overexpression of the KAS Ⅲ-like gene YxwZ3 increases carotenoids production in Aurantiochytrium sp. SZU445

Industrial Crops and Products, Journal Year: 2022, Volume and Issue: 187, P. 115435 - 115435

Published: Aug. 2, 2022

Identifying gene that enhances specific product accumulation of industrial strains is a central tenet metabolic engineering. Aurantiochytrium sp. has come into the limelight by virtue their biotechnological importance in production bioactive compounds, including fatty acids and carotenoids. However, there restrict high yield carotenoids This study, KAS Ⅲ-like enzyme 'YxwZ3′ were successfully used to construct recombinant cassette. We utilized TEF1 EM7 promoters overexpress YxwZ3 SZU445. The transformed strain displayed 4.88-fold increase total compared wild-type strain, involved β-carotene (37.96 μg/g), phytoene (1.55 β-cryptoxanthin (1.57 antheraxanthin (0.02 α-cryptoxanthin (0.25 μg/g) echinenone (0.72 with downregulated. It speculated more malonyl-CoA was MVA pathway for synthesis based on overexpression instead acid pathway. In addition, content lines increased 1.33 times (56.52 through optimization fermentation condition response surface methodology. These findings demonstrated significant impact sp., provided theoretical basis constructing high-yielding carotenoid-producing strains.

Language: Английский

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Plant synthetic biology for human health: advances in producing medicines in heterologous expression systems

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103142 - 103142

Published: May 11, 2024

Plant synthetic biology has the capability to provide solutions global challenges in production and supply of medicines. Recent advances 'omics' technologies have accelerated gene discoveries medicinal plant research so that even multistep biosynthetic pathways for bioactive natural products with high structural complexity can be reconstituted heterologous expression systems more rapidly. This review provides an overview concept strategies used produce high-value highlights recent successes engineering biosynthesis conventional new medicines alternative hosts.

Language: Английский

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Golden Rice—Lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2022, Volume and Issue: unknown, P. 1 - 29

Published: Jan. 1, 2022

Language: Английский

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Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds

aBIOTECH, Journal Year: 2021, Volume and Issue: 2(3), P. 191 - 214

Published: May 18, 2021

Staple grains with low levels of provitamin A carotenoids contribute to the global prevalence vitamin deficiency and therefore are main targets for biofortification. However, carotenoid stability during both seed maturation postharvest storage is a serious concern full benefits biofortified grains. In this study, we utilized Arabidopsis as model establish biofortification strategies in seeds. We discovered that manipulation biosynthetic activity by seed-specific expression Phytoene synthase (PSY) increases total but increased prone degradation storage, consistent previous studies contrast, stacking Orange (ORHis ), gene initiates chromoplast biogenesis, dramatically enhances content stability. Up 65- 10-fold β-carotene carotenoids, respectively, composing over 63% were observed seeds containing ORHis PSY. Co-expression Homogentisate geranylgeranyl transferase (HGGT) PSY further accumulation storage. Moreover, knocking-out hydroxylase 2 (BCH2) CRISPR/Cas9 not only potentially facilitates also minimizes negative effect production on germination. Our findings provide new insights into various processes multiplexed strategy simultaneously target biosynthesis, turnover, stable crop seeds.The online version contains supplementary material available at 10.1007/s42994-021-00046-1.

Language: Английский

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Plastid diversity and chromoplast biogenesis in differently coloured carrots: role of the DcOR3Leu gene

Planta, Journal Year: 2022, Volume and Issue: 256(6)

Published: Oct. 29, 2022

Language: Английский

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