Exosomes from Bone Marrow Mesenchymal Stem Cells with Overexpressed Nrf2 Inhibit Cardiac Fibrosis in Rats with Atrial Fibrillation

Cardiovascular Therapeutics, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 15

Published: March 15, 2022

Even though nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling has been associated with the pathogenesis of multiple heart conditions, data on roles Nrf2 within atrial fibrillation (AF) still remain scant. The present investigation had aim analyzing Nrf2-overexpressing role/s upon bone mesenchymal stem cell- (BMSC-) derived exosomes in rats AF.Exosomes were collected from control or lentivirus-transduced BMSCs and then injected into AF through tail vein. duration was observed using electrocardiography. Immunohistochemical staining employed for assessing Nrf2, HO-1, α-SMA, collagen I, TGF-β1 expression profiles myocardium tissues. Conversely, Masson utilized to evaluate fibrosis whereas apoptosis myocardia evaluated TUNEL assays. In addition, TNF-α, IL-1β, IL-4, IL-10 serum assessed ELISA.Results current study showed significant downregulation Nrf2/HO-1 rat myocardia. It found that injection Lv-Nrf2 significantly alleviated lowered timespans together reducing cardiomyocyte apoptosis. Moreover, essentially AF-driven also inhibited inflammatory responses AF.Delivery BMSC-derived overexpressed AF-induced arrhythmias, myocardial fibrosis, apoptosis, inflammation via pathway triggering.

Language: Английский

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Vitamin D as therapeutic modulator in cerebrovascular diseases: a mechanistic perspectives

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 63(25), P. 7772 - 7794

Published: March 14, 2022

Vitamin D deficiency has been linked to several major chronic diseases, such as cardiovascular and neurodegenerative diabetes, cancer, oxidative stress, inflammation, aging. appears be particularly harmful the system, it can cause endothelial dysfunctioning vascular abnormalities through modulation of various downstream mechanisms. As a result, new research indicates that therapeutic approaches targeting vitamin inadequacies or its significant effects, impaired autophagy, abnormal pro-inflammatory pro-oxidant reactions, may delay onset severity cerebrovascular disorders stroke neurologic malformations. modulates molecular pathways, i.e., Nitric Oxide, PI3K-Akt Pathway, cAMP pathway, NF-kB Sirtuin 1, Nrf2, FOXO, in disorder. The current review shows evidence for D's mitigating slowing progression these disorders, which are causes disability death worldwide.

Language: Английский

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β-aminoisobutyrics acid, a metabolite of BCAA, activates the AMPK/Nrf-2 pathway to prevent ferroptosis and ameliorates lung ischemia-reperfusion injury

Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(1)

Published: Dec. 4, 2023

Abstract Background Lung ischemia-reperfusion (I/R) injury is a serious clinical problem without effective treatment. Enhancing branched-chain amino acids (BCAA) metabolism can protect against cardiac I/R injury, which may be related to bioactive molecules generated by BCAA metabolites. L-β-aminoisobutyric acid (L-BAIBA), metabolite of BCAA, has multi-organ protective effects, but whether it protects lung unclear. Methods To assess the effect L-BAIBA an animal model was clamping hilum left lung, followed releasing clamp in C57BL/6 mice. Mice with were pre-treated or post-treated (150 mg/kg/day), given gavage intraperitoneal injection. assessed measuring edema and analyzing blood gases. Inflammation proinflammatory cytokines bronchoalveolar lavage fluid (BALF), neutrophil infiltration measured myeloperoxidase activity. Molecular biological methods, including western blot immunofluorescence, used detect potential signaling mechanisms A549 BEAS-2B cells. Results We found that from inhibiting ferroptosis, depends on up-regulation expressions GPX4 SLC7A11 Additionally, we demonstrated Nrf-2 pathway key inhibitory ferroptosis induce nuclear translocation Nrf-2. Interfering expression eliminated ferroptosis. A screening pathways revealed increase phosphorylation AMPK, compound C block induced L-BAIBA. The presence also blocked effects Conclusions Our study showed via AMPK/Nrf-2 pathway, could therapeutic target.

Language: Английский

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CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway

Experimental Cell Research, Journal Year: 2024, Volume and Issue: 440(1), P. 114127 - 114127

Published: June 12, 2024

Language: Английский

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Pharmacological mechanisms by which baicalin ameliorates cardiovascular disease

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 9, 2024

Baicalin is a flavonoid glycoside obtained from the dried root of

Language: Английский

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