bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 4, 2022

Abstract Cystic echinococcosis (CE) is one of the most widespread and harmful zoonotic parasitic diseases it commonly affects liver. In this study, we characterized multiple changes in mouse hepatocytes following treatment with excretory-secretory (ES) products Echinococcus granulosus protoscoleces by a factorial experiment. The cell counting kit-8 assay (CCK-8), 5-ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry were used to detect growth hepatocytes. Inverted microscopy, scanning electron microscopy (SEM), transmission (TEM) observe morphology ultrastructure An automatic biochemical analyzer an ELISA detection kit determine six conventional hepatocyte enzymatic indices, levels five hepatocyte-synthesized substances, contents glucose lactate. Western blot analysis was conducted analyze protein expression rate-limiting enzymes metabolism pathway hepatocytes: glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGT), leucine arylamidase (LAP). results CCK-8 EdU assays both showed that ES could inhibit proliferation hepatocytes, indicated promote apoptosis After treatment, disrupted certain extent. membrane microvilli observed through SEM, nucleus, mitochondria, rough endoplasmic reticulum TEM. activities increased addition Fe synthesis albumin (ALB), uric acid (UA), urea, whereas transferrin (TRF) decreased. all key decreased, biological effects significantly inhibited. We analyzed causes possible complications caused various advocate corresponding measures. also propose mechanisms which cause necrosis, but specific mechanism requires further study. Author Summary , widely distributed infected tapeworm, has serious economic social burdens pastoral areas China. metacestodes mainly infect liver intermediate hosts (humans, cattle, sheep, etc.). Currently, on hepatocytes’ ultrastructure, enzymology, function, have not been characterized, accurate characterization crucial study related pathogenesis preventive therapy. Here, characterize using action found inhibited promoted apoptosis. can degree damage membrane, indexes elevated, they ALT, AST, LDH, ALP, GGT, LAP, Fe, ALB, UA, urea synthesized higher TRF lower. Reduced pathways including PFK-1, IDH, G-6-PD, GS, GP, GLUT-2, indicating inhibits Our only detail proposed causing these effects, provided basis for subsequent studies prevention, treatment.

Language: Английский