Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 322 - 322

Published: March 6, 2024

Cryopreservation is a stressful process for sperm, as it associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create imbalance antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the LPO fragmentation frozen–thawed horse sperm relies upon activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) paraoxonase type 1 (PON1)); non-enzymatic capacity (Trolox-equivalent (TEAC), plasma ferric reducing (FRAP) cupric (CUPRAC)); oxidative stress index (OSI) their seminal (SP). Based on total motility integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups good- (GFEs) poor-(PFEs) freezability ejaculates. (global breaks) higher 0.05) PFE group than GFE group, thawing showing positive relationship SP OSI levels production. In addition, negatively correlated activity antioxidants (PON1 TEAC). The present results indicate that vary between These differences could from variations TEAC) balance oxidant components SP.

Language: Английский

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Cryopreservation of tissues and organs: present, bottlenecks, and future

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: May 25, 2023

Tissue and organ transplantation continues to be an effective measure for saving the lives of certain critically ill patients. The preservation methods that are commonly utilized in clinical practice presently only capable achieving short-term storage, which is insufficient meeting demand transplantation. Ultra-low temperature storage techniques have garnered significant attention due their capacity long-term, high-quality tissues organs. However, experience cryopreserving cells cannot readily extrapolated cryopreservation complex organs, latter still confronts numerous challenges its application. This article summarizes current research progress cryogenic discusses limitations existing studies main obstacles facing finally introduces potential directions future efforts.

Language: Английский

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Antioxidants and Oxidants in Boar Spermatozoa and Their Surrounding Environment Are Associated with AMPK Activation during Liquid Storage

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(3), P. 214 - 214

Published: March 10, 2023

Activation of the AMP-activated protein kinase (AMPK) has been demonstrated to be beneficial for boar sperm quality and functionality, while underlying mechanism AMPK activation spermatozoa remains obscure. This study aimed explore effect antioxidants oxidants in their surrounding fluid (SF) on during liquid storage. Ejaculates from Duroc boars, routinely used semen production, were collected diluted a final concentration 25 × 106/mL. In experiment 1, twenty-five samples eighteen boars stored at 17 °C 7 days. 2, three pooled created nine ejaculates used, each sample was treated with 0, 0.1, 0.2, 0.4 μM/L H2O2 3 h. Sperm SF, intracellular AMP/ATP ratio, expression levels phosphorylated (Thr172) determined. significantly decreased storage time terms viability (p < 0.05). Antioxidant oxidant markedly affected time, decline SF total antioxidant capacity (TAC) 0.05), malondialdehyde (MDA) sperm's status (TOS), as well fluctuation superoxidase dismutase-like (SOD-like) activity The ratio increased 0.05) day 4 subsequently its lowest value days 6 2 Correlation analyses indicate that correlated which phosphorylation Treatment induced damages (SF TAC, p 0.05; SOD-like activity, 0.01), an increase MDA, ROS higher comparison control. results suggest are involved

Language: Английский

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Cellular and Molecular Consequences of Stallion Sperm Cryopreservation: Recent Approaches to Improve Sperm Survival

Journal of Equine Veterinary Science, Journal Year: 2023, Volume and Issue: 126, P. 104499 - 104499

Published: April 25, 2023

Language: Английский

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Cryopreservation of Horse Sperm

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 193 - 206

Published: Jan. 1, 2025

Language: Английский

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Thawing of cryopreserved sperm from domestic animals: Impact of temperature, time, and addition of molecules to thawing/insemination medium

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 268, P. 107572 - 107572

Published: Aug. 3, 2024

Language: Английский

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BIOCHEMICAL MARKERS OF STALLION SPERM QUALITY (review)

Sel skokhozyaistvennaya Biologiya, Journal Year: 2023, Volume and Issue: 58(2), P. 249 - 259

Published: April 1, 2023

БИОХИМИЧЕСКИЕ МАРКЕРЫ КАЧЕСТВА СПЕРМЫ ЖЕРЕБЦОВ * (обзор) М.М.АТРОЩЕНКО  , Д.В.МЕДВЕДЕВ Семенная плазма представляет собой многокомпонентную жидкость, служащую средством доставки сперматозоидов к ооциту.Она не только транспортирует мужские гаметы, но и обеспечивает их защиту питание во время дальнейшего движения в женских половых путях (T.R. Talluri с соавт., 2017).Сведения о воздействии компонентов семенной плазмы на половые клетки, а также поиск маркеров криоустойчивости фертильности спермы несомненный интерес для исследователей.Поскольку продолжительность жизни жеребцов, способность капацитации фертильность сильно различаются у разных особей, коневодства важно исследовать факторы

Language: Русский

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Relationship of Aquaporin-3 and Aquaporin-7 with Cryotolerance of Angus Bull Spermatozoa

Pakistan Veterinary Journal, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Semen of all bulls is not suitable for freezing; similarly, ejaculates with excellent sperm vitality are freezing.Based on survival at post-thawing, bull categorized as good freezability (GFE) and poor (PFE).In this study, quality parameters GFE PFE, the localization aquaporin-3 (AQP-3) aquaporin-7 (AQP-7) were investigated.Moreover, relationship AQP-3 AQP-7 cryotolerance sperms was recorded.After fresh semen evaluation, 24 from four Angus cryopreserved their analyzed by AndroVision before after frozen-thawed.Expression determined through Western blot, while detected immunocytochemistry.The PFE well differentiated motility, progressive viability, acrosome integrity, mitochondrial activity, except DNA integrity.AQP-3 significantly higher in than frozen-thawed sperm, that (P<0.05).The location seen mid-piece, recorded post-acrosome region, mid prime tailpiece.In conclusion, PGE can be distinguished expression semen, could a biomarker sperm.This study hoped to improve efficiency frozen production provide reference improvement technology other male animals, humans.

Language: Английский

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Physiological evaluation of seasonal sperm quality in a biannual spawner, Pacific abalone: Effects on in-vitro fertilization and cryotolerance

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 269, P. 115809 - 115809

Published: Dec. 12, 2023

Pacific abalone, Haliotis discus hannai, is a highly valuable gastropod mollusk commonly found in Southeast Asia. The present study aims to analyze the seminal plasma quality, sperm and cryotolerance of abalone during its reproductive season. quality was evaluated by analyzing biochemical metabolite composition, enzymatic activity (superoxide dismutase, catalase, glutathione), lipid peroxidation (LPO) activity. motility, concentration, volume, ATP content, acrosome integrity (AI), membrane (PMI), mitochondrial potential (MMP), DNA integrity, fertilization potential. capacity post-thaw AI, PMI, MMP, integrity. Seminal osmolarity significantly higher (1123.3 ± 1.5 mOsmL

Language: Английский

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Homologous seminal plasma efficiently activates epididymal tail sperm compared to traditional tris buffer and sperm-TALP in sheep

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: April 2, 2024

Abstract We hypothesized that ram epididymal tail sperm may be efficiently activated in homologous seminal plasma compared to tris and sperm-TALP. Eighty ejaculates were collectedfrom five healthy fertile rams by artificial vagina. Ejaculates with ≥ 3 mass motility 70% initial score considered pooled. The was harvested two-phase centrifugation (A-3000 g, 4 °C, 20 min; B-3600 30 min). Sperm collected dissecting into equal parts which placed three small (35 mm) petri dishes for activation (SP), sperm-TALP (TP) buffer (TR). quality assessed at 0, 24, 48 72 h of cold storage on the basis motility, viability, HOST acrosomal integrity. In addition subjective assessment integrity viability evaluated using molecular florescent probe combinations-fluorescein isothiocyanate (FITC) conjugated peanut agglutinin (PNA) plus propidium iodide (PI) carboxyflorescene diacetate (CFDA) iodide, respectively. Motility, CFDA positive (Viable) reacted percentage significantly higher (p < 0.05) SP both TP TR storage. FITC-PNA negative (Intact acrosomes) did also differ between SP, various hours conclusion, preserved This study provides an opportunityto further explore role cryoprotection fertilizing capacity sperm.

Language: Английский

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