Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 12, 2023
Abstract
We
hypothesized
that
ram
epididymal
tail
sperm
may
be
efficiently
activated
in
homologous
seminal
plasma
compared
to
tris
and
sperm-TALP.
Eighty
ejaculates
were
collected
from
five
healthy
fertile
rams
by
artificial
vagina
for
harvesting
of
two-phase
centrifugation.
Spermatozoa
three
activation
fluids-
(SP),
sperm-TALP
(TP)
buffer
(TR)
preserved
at
4°C
72
hours.
Sperm
motility
was
higher
(p<0.05)
SP
(64.33±4.80-48
hr;
52.50±2.14-72
hr)
both
TP
(51.66±2.10-48
40.83±3.00-72
TR
groups
(52.50±4.03-48
41.66±3.33-72
48
hr
cold
storage.
CFDA
(Carboxy
fluorescein
diacetate)
positive
(Viable)
percentage
also
than
(72.39±0.62-SP,
67.13±1.29-TP,
66.89±1.65-TR)
hours
(63.37±2.19-SP,
56.13±1.60-TP,
58.18±1.49-TR)
HOST
reacted
(65.10±1.55-SP,
59.95±0.24-TP,
61.50±1.11-TR)
(55.09±1.17-SP,
46.53±0.78-TP,
50.16±0.16-TR)
storage
groups.
FITC-PNA
(Fluorescein
isothiocyanate
conjugated
peanut
agglutinin)
negative
(Intact
acrosomes)
0
(91.66±0.90-SP,
88.13±1.02-TR),
(75.54±0.70-SP,
74.49±1.07-TP)
(68.27±1.15-SP,
61.97±1.35-TP,
62.91±1.53-TR).
In
conclusion,
This
study
opened
a
new
window
research
further
explore
the
role
cryoprotection
sperm.
Animals,
Journal Year:
2024,
Volume and Issue:
14(17), P. 2465 - 2465
Published: Aug. 25, 2024
Sensitivity
to
freezing
remains
a
critical
issue
in
stallion
semen
cryopreservation
procedures.
To
explore
this
topic
in-depth,
was
collected
from
ten
stallions,
diluted
with
three
different
extenders,
transported
the
laboratory,
and
then
centrifuged
frozen
four
extenders.
We
conducted
analyses
of
sperm
kinetics,
mitochondrial
membrane
potential
(MMP),
hydrogen
peroxide
content
both
before
after
freezing.
Additionally,
we
assessed
antioxidant
activity
using
ABTS
FRAP
methods
measured
nitric
oxide
stable
metabolites
(NOx)
blank
seminal
plasma,
extenders
conditioned
by
spermatozoa
found
significant
variability
NOx
plasma.
In
ABTS-based
values
were
correlated
some
kinematic
parameters
MMP
refrigerated
semen,
while
no
correlation
observed
parameters.
Sperm
function
varied
significantly
between
stallions
but
not
either
or
However,
differences
activities
among
following
These
results
provide
new
insights
into
factors
contributing
individual
stallions'
tolerance
Czech Journal of Animal Science,
Journal Year:
2024,
Volume and Issue:
69(11), P. 450 - 461
Published: Nov. 8, 2024
Dietary
antioxidant
supplementation
has
become
popular
for
managing
male
infertility
because
of
its
impact
on
the
capacity
semen.
This
study
evaluates
effect
dietary
seminal
characteristics
Colombian
Creole
horses.
Semen
from
six
stallions
was
collected
using
an
artificial
vagina.
The
were
randomly
allocated
to
a
control
group
(CG)
or
(SG)
that
received
multi-antioxidant
supplement.
A
repeated
measures
design,
with
wash
out
period
two
weeks
between
treatments,
used.
Sperm
motility
and
kinematics
assessed
computer-assisted
semen
analysis
system,
membrane
integrity
(MI)
acrosomal
(AI)
sperm
fluorescent
microscopy,
abnormal
morphology
(AM)
analysed
supravital
staining,
functionality
hypoosmotic
swelling
(HOS)
test.
fraction
each
ejaculate
sample
centrifuged
obtain
plasma
(SP).
reactive
oxygen
species
total
(TAC)
SP
measured
via
spectrofluorimetry.
Linear
models
fitted,
comparison
means
performed
Tukey's
Dietary
increased
beat
cross
frequency.
In
addition,
higher
AI
found
SG.
Supplementation
also
TAC
SP.
Individual
revealed
progressive
horses
in
SG;
however,
reduced
these
parameters
other
individuals
compared
CG.
It
can
be
concluded
nutraceutical
provides
vitamins,
minerals,
amino
acids,
omega-3
increase
quality
as
well
SP;
this
may
subject
individual
horse.
Cryopreservation
is
a
stressful
process
for
sperm
as
it
associated
with
an
increased
production
of
reactive
oxygen
species
(ROS).
Elevated
ROS
levels,
which
create
imbalance
antioxidant
capacity,
may
result
in
membrane
lipid
peroxidation
(LPO),
protein
damage
and
DNA
fragmentation.
This
study
aimed
to
determine
whether
LPO
fragmentation
frozen-thawed
horse
relies
upon
the
activity,
including
enzymes
(superoxide
dismutase
(SOD),
glutathione
peroxidase
(GPX),
catalase
(CAT),
paraoxonase
type
1
(PON1));
non-enzymatic
capacity
(Trolox
equivalent
(TEAC),
plasma
ferric
reducing
(FRAP),
cupric
(CUPRAC));
oxidative
stress
index
(OSI),
their
seminal
(SP).
Based
on
total
motility
integrity
(SYBR14+/PI−)
after
thawing,
ejaculates
were
hierarchically
(p
&lt;
0.001)
clustered
into
two
groups
good
(GFE)
poor
(PFE)
freezability.
(global
breaks)
higher
0.05)
PFE
than
GFE,
thawing
showing
positive
relationship
SP-OSI
levels
production.
In
addition,
negatively
correlated
activity
SP
antioxidants
(PON1
TEAC).
The
present
results
indicate
that
vary
between
ejaculates.
These
differences
could
from
variations
TEAC)
balance
oxidant
components
SP.
Sel skokhozyaistvennaya Biologiya,
Journal Year:
2023,
Volume and Issue:
58(2), P. 249 - 259
Published: April 1, 2023
БИОХИМИЧЕСКИЕ
МАРКЕРЫ
КАЧЕСТВА
СПЕРМЫ
ЖЕРЕБЦОВ
*
(обзор)
М.М.АТРОЩЕНКО
,
Д.В.МЕДВЕДЕВ
Семенная
плазма
представляет
собой
многокомпонентную
жидкость,
служащую
средством
доставки
сперматозоидов
к
ооциту.Она
не
только
транспортирует
мужские
гаметы,
но
и
обеспечивает
их
защиту
питание
во
время
дальнейшего
движения
в
женских
половых
путях
(T.R.
Talluri
с
соавт.,
2017).Сведения
о
воздействии
компонентов
семенной
плазмы
на
половые
клетки,
а
также
поиск
маркеров
криоустойчивости
фертильности
спермы
несомненный
интерес
для
исследователей.Поскольку
продолжительность
жизни
жеребцов,
способность
капацитации
фертильность
сильно
различаются
у
разных
особей,
коневодства
важно
исследовать
факторы
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 12, 2023
Abstract
We
hypothesized
that
ram
epididymal
tail
sperm
may
be
efficiently
activated
in
homologous
seminal
plasma
compared
to
tris
and
sperm-TALP.
Eighty
ejaculates
were
collected
from
five
healthy
fertile
rams
by
artificial
vagina
for
harvesting
of
two-phase
centrifugation.
Spermatozoa
three
activation
fluids-
(SP),
sperm-TALP
(TP)
buffer
(TR)
preserved
at
4°C
72
hours.
Sperm
motility
was
higher
(p<0.05)
SP
(64.33±4.80-48
hr;
52.50±2.14-72
hr)
both
TP
(51.66±2.10-48
40.83±3.00-72
TR
groups
(52.50±4.03-48
41.66±3.33-72
48
hr
cold
storage.
CFDA
(Carboxy
fluorescein
diacetate)
positive
(Viable)
percentage
also
than
(72.39±0.62-SP,
67.13±1.29-TP,
66.89±1.65-TR)
hours
(63.37±2.19-SP,
56.13±1.60-TP,
58.18±1.49-TR)
HOST
reacted
(65.10±1.55-SP,
59.95±0.24-TP,
61.50±1.11-TR)
(55.09±1.17-SP,
46.53±0.78-TP,
50.16±0.16-TR)
storage
groups.
FITC-PNA
(Fluorescein
isothiocyanate
conjugated
peanut
agglutinin)
negative
(Intact
acrosomes)
0
(91.66±0.90-SP,
88.13±1.02-TR),
(75.54±0.70-SP,
74.49±1.07-TP)
(68.27±1.15-SP,
61.97±1.35-TP,
62.91±1.53-TR).
In
conclusion,
This
study
opened
a
new
window
research
further
explore
the
role
cryoprotection
sperm.
