Nutrient sensing signaling and metabolic responses in shrimp Litopenaeus vannamei under acute ammonia stress

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 253, P. 114672 - 114672

Published: Feb. 22, 2023

Ammonia is the primary environmental factor affecting growth and health of crustaceans. It would induce oxidative stress metabolic disorders. Extra amount energy was demanded to maintain physiological functions under ammonia stress. However, limited information available on its effects main nutrient metabolism, as well sensing signaling pathways. In present study, shrimp Litopenaeus vannamei were exposed acute injected with amino acid solution. The results showed that exposure resulted in lower free levels hemolymph, incomplete activation mechanistic target rapamycin (mTOR) cascaded less protein synthesis muscle. induced autophagy activated AMP-activated kinase (AMPK) pathway. Meanwhile, enhanced glycolysis lipogenesis, but inhibited lipolysis. characterized integrated responses provides critical clues understand performance

Language: Английский

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Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 21

Published: Jan. 5, 2024

Plants could be used for multiple medicinal purposes. Moringa oleifera (MO) is considered the most famous plant this purpose. The present review aimed to spot light on recent medicinal, biochemical, and nutritional applications of MO. contains a huge number nutrients such as fatty acids, amino proteins, polysaccharides, minerals, vitamins. It has been control glucose, lipids, vitamins, hormones, antioxidants in many animals human models. Its are also varied wide; it manage lots disorders. Extracts isolated ingredients from open way researchers all over world study its biochemical impact experimental clinical spots significance MO opening discussion demonstrating strengths weaknesses research area. Regardless contrary concept, we promising that studied both molecular levels. We recommended further effects healthy diseased

Language: Английский

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Preliminary Phytochemical Screening and Antioxidant Activity of Commercial Moringa oleifera Food Supplements

Antioxidants, Journal Year: 2023, Volume and Issue: 12(1), P. 110 - 110

Published: Jan. 2, 2023

Moringa oleifera has been reported to possess a high number of bioactive compounds; hence, several food supplements are commercially available based on it. This work aimed analyze the phytochemical composition and antioxidant activity commercial supplements. The phenolic methanolic extracts was determined by using high-performance liquid chromatography with diode-array electrospray ionization mass spectrometric detection (HPLC-DAD-ESI-MSn), assessed ABTS·+ DPPH assays. Thirty-three compounds were identified, all main quantified, observing that contribution profile due kaempferol quercetin glucosides. in both assays agreed content: higher levels, activity. obtained results compared those previously published regarding leaves establish potential benefits supplement consumption diet.

Language: Английский

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Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

Biomolecules & Therapeutics, Journal Year: 2024, Volume and Issue: 32(3), P. 349 - 360

Published: April 11, 2024

Oxidative stress contributes to the onset of chronic diseases in various organs, including muscles. Morroniside, a type iridoid glycoside contained

Language: Английский

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Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1576 - 1576

Published: Aug. 7, 2023

Loss of skeletal muscle mass and function has detrimental effects on quality life, morbidity, mortality, is particularly relevant in aging societies. The enhancement mitochondrial shown promise promoting differentiation function. Ginsenoside Rc (gRc), a major component ginseng, various pharmacological activities; however, its effect loss remains poorly explored. In this study, we examined the gRc hydrogen peroxide (H2O2)-induced reduction cell viability C2C12 myoblasts myotubes H2O2-induced myotube degradation. addition, investigated production intracellular reactive oxygen species (ROS) superoxide, ATP generation, peroxisome proliferator-activated receptor-gamma co-activator 1α (PGC-1α) activity under H2O2 treatment. Furthermore, to elucidate mechanism action gRc, conducted transcriptome analysis treated with or without effectively suppressed cytotoxicity, ROS, superoxide production, restored PGC-1α promoter activity, increased synthesis. Moreover, significantly affected expression levels genes involved maintaining biogenesis, while downregulating associated degradation oxidative stress. We provide compelling evidence supporting potential as promising treatment for weakness. Further investigations pathological conditions will contribute clinical development therapeutic intervention.

Language: Английский

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The impact of physical activity on promoter-specific methylation of genes involved in the redox-status and disease progression: A longitudinal study on post-surgery female breast cancer patients undergoing medical treatment

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103033 - 103033

Published: Jan. 5, 2024

Most anticancer treatments act on oxidative-stress pathways by producing reactive oxygen species (ROS) to kill cancer cells, commonly resulting in consequential drug-induced systemic cytotoxicity. Physical activity (PA) has arisen as an integrative therapy, having positive health effects, including redox-homeostasis. Here, we investigated the impact of online supervised PA program promoter-specific DNA methylation, and corresponding gene expression/activity, 3 antioxidants- (SOD1, SOD2, CAT) breast (BC)-related genes (BRCA1, L3MBTL1 RASSF1A) a population-based sample women diagnosed with primary BC, undergoing medical treatment. We further examined mechanisms involved methylating demethylating pathways, predicted biological interactions exercise-modulated molecules, functional relevance modulated antioxidant markers parameters related aerobic capacity/endurance, physical fatigue quality life (QoL). maintained levels SOD blood plasma, at cellular level significantly increased SOD2 mRNA (≈+77 %), contrary their depletion due This change was inversely correlated methylation promoter (≈-20 %). Similarly, found significant effect only (≈-25 which its (≈+43 Finally, TET1 (≈+15 %) decreased expression DNMT3B (≈-28 Our results suggest that PA-modulated affects several signalling pathways/biological activities oxidative stress response, chromatin organization/regulation, DNA/protein binding. These changes may positively clinical outcomes improve response treatment post-surgery BC patients.

Language: Английский

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Ficus lindsayana Leaf Extract Protects C2C12 Mouse Myoblasts Against the Suppressive Effects of Bisphenol-A on Myogenic Differentiation

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 476 - 476

Published: Jan. 8, 2025

Recently, toxicological and epidemiological research has provided strong support for the unfavorable effects of bisphenol-A (BPA, 2,2'-bis(4-hydroxyphenyl) propane) on myogenesis its underlying mechanisms. Researchers have therefore been looking new strategies to prevent or mitigate these injurious BPA human body. It found that plant extracts may act as potential therapeutic agents functional foods, preventing diseases caused by BPA. We previously reported Ficus lindsayana (FL) extract exhibits anti-inflammation activity in macrophages via suppressing expression inflammation-related molecules anti-insulin resistance inflammation-treated adipocytes. In this study, we investigated whether leaf (FLLE) protects C2C12 mouse myoblasts against suppressive myogenic differentiation. The viability BPA-stimulated was significantly increased when co-treated with FLLE (200 µg/mL), suggesting lessen inhibitory cell division. also neo-myotube formation inducing fusion into multinucleated myotubes compared BPA-treated control cells, without impacting viability. addition, levels myogenin myocyte enhancer factor 2A (MEF2A), which are crucial markers regulators myogenesis, were markedly addition (50 µg/mL) cells. This finding suggests effectively improved differentiation BPA-exposed myoblasts. treatment raised total Akt protein enhancing phosphorylation. obviously phosphorylation p70S6K 4E-BP1, key downstream targets Akt/mTOR signaling cascade, elevating 4E-BP1 levels. These results suggest diminishes decline induced regulation differentiation-related pathway. information obtained from study demonstrates health benefits plant, warrants further investigation an alternative medicine, ingredient, food supplement can negative other toxicants.

Language: Английский

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Nutritional Status and Reproductive Performance Evaluations in Birds Fed Diets Incorporated with Moringa oleifera Leaves and Seeds

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(3)

Published: March 1, 2025

Introduction : This research assessed the impact of adding Moringa oleifera leaf and seed to diet laying hens on their nutritional status reproductive performance. Methods: Twenty one (21) week-old were randomly divided into seven groups three birds. The control group was fed Layers’ Mash, whereas experimental given a supplemented with 5%, 10%, or 20% leaves seeds. feeding lasted six weeks. Eggs, meat blood samples analysed for quality evaluation effect some common hormones using appropriate standard protocols. Results: present study revealed statistically significant (P < 0.05) differences in internal exterior properties chicken eggs across groups. Compared those group, concentrations serum follicle stimulating hormone (FSH) luteinising found increase at supplementation doses. increases total protein, albumin, ferritin, vitamins, certain minerals detected different concentrations. (p changes dry matter content, energy ash ether extract albumen height bird haematological parameters detected. Conclusion: addition birds condition performance, making it highly recommended.

Language: Английский

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Nicotinamide mononucleotide ameliorates hypertriglyceridemia pancreatitis via NAD+/SIRT1-mediated TXNIP suppression and NOTCH pathway for accelerated repair-associated processes

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114620 - 114620

Published: April 10, 2025

Language: Английский

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Eco-Friendly Synthesis of Zirconia Nanoparticles Using Sonchus asper Extract: A Sustainable Approach to Enhancing Chinese Cabbage Growth and Remediating Chromium-Contaminated Soil

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 324 - 324

Published: April 22, 2025

Chromium (Cr) contamination poses severe risks to plant health and soil quality, requiring sustainable remediation methods. This study explored the synthesis of zirconia nanoparticles (PF-ZrO2 NPs) from Sonchus asper extract assessed their potential alleviate Cr toxicity in Chinese cabbage (Brassica rapa var. pekinensis). The characterization was performed through XRD, SEM, FTIR analyses, confirming crystalline nature, structure, surface chemistry. results indicated significant declines growth, chlorophyll content, biomass, nutrient uptake under stress (treatments T2 T4), accompanied by elevated oxidative indicators (H2O2, MDA) accumulation. application PF-ZrO2 NPs (T3 T5) notably reduced shoot concentrations (by 58.94% 35.90%) improved level 5.41% 14.41%). Additionally, increased antioxidant enzyme activity (SOD, POD, CAT) properties (pH, cation exchange capacity, retention). These findings suggest green-synthesized are effective, environmentally friendly candidates for contaminated soils.

Language: Английский

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