Membranes,
Journal Year:
2022,
Volume and Issue:
12(10), P. 905 - 905
Published: Sept. 20, 2022
Carotenoids
are
potent
antioxidants
with
a
wide
range
of
biomedical
applications.
However,
their
delivery
into
human
cells
is
challenging
and
relatively
inefficient.
While
the
use
natural
water-soluble
carotenoproteins
capable
to
reversibly
bind
carotenoids
transfer
them
membranes
promising,
quantitative
estimation
remains
unclear.
In
present
work,
we
studied
echinenone
(ECN)
by
cyanobacterial
carotenoprotein
AnaCTDH
(C-terminal
domain
homolog
Orange
Carotenoid
Protein
from
Anabaena),
liposome
labelled
BODIPY
fluorescent
probe.
We
observed
that
addition
AnaCTDH-ECN
liposomes
led
significant
changes
in
fast-kinetic
component
fluorescence
decay
curve,
pointing
on
dipole-dipole
interactions
between
probe
ECN
within
membrane.
It
may
serve
as
an
indirect
evidence
To
study
detail,
carried
out
molecular
dynamics
modeling
localization
lipid
bilayer
calculate
its
orientation
factor.
Next,
exploited
FRET
assess
concentration
delivered
AnaCTDH.
Finally,
used
time-resolved
anisotropy
microviscosity
liposomal
membranes.
Incorporation
β-carotene
increased
membrane
while
effect
astaxanthin
mono-
diester
forms
was
less
pronounced.
At
temperatures
below
30
°C
concentration-dependent
manner,
supporting
protein-mediated
carotenoid
mechanism.
Combining
all
data,
propose
FRET-based
analysis
assessment
approaches
characterize
efficiency
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2257 - 2257
Published: Feb. 13, 2024
Bioinert
materials
such
as
the
zirconium
dioxide
and
aluminum
oxide
are
widely
used
in
surgery
dentistry
due
to
absence
of
cytotoxicity
relation
surrounding
cells
body.
However,
little
attention
has
been
paid
study
metabolic
processes
occurring
at
implant–cell
interface.
The
activity
mouse
3T3
fibroblasts
incubated
on
yttrium-stabilized
ceramics
cured
with
(ATZ)
stabilized
(Y-TZP)
was
analyzed
based
ratio
free/bound
forms
cofactors
NAD(P)H
FAD
obtained
using
two-photon
microscopy.
results
show
that
demonstrate
a
shift
towards
free
form
NAD(P)H,
which
is
observed
during
glycolysis
process,
which,
according
our
assumptions,
related
porosity
surface
ceramic
structures.
Consequently,
despite
high
viability
good
proliferation
assessed
an
MTT
test
scanning
electron
microscope,
state
hypoxia
incubation
FLIM
this
work
can
be
additional
information
for
scientists
who
interested
manufacturing
osteoimplants.
Journal of Raman Spectroscopy,
Journal Year:
2023,
Volume and Issue:
54(5), P. 487 - 500
Published: Jan. 30, 2023
Abstract
Skin
surface
lipid
(SSL)
film
is
a
mixture
of
sebum
and
epidermal
lipids
protecting
the
skin
from
effects
environment
such
as
solar
radiation.
SSLs
are
suitable
target
scavenger
for
all
reactive
oxygen
species
(ROS)
generated
in
skin.
In
SSLs,
vitamin
E
has
been
identified
one
main
antioxidants
that
can
capture
ROS.
This
paper
third
part
global
study
evaluates
effect
radiation
on
classes
constitute
SSLs.
first
two
parts,
fatty
acids
triglycerides
was
studied
using
vibrational
spectroscopy.
this
part,
other
squalene
(SQ),
cholesterol
(Chol),
wax
ester
(WE)
(Palmitoleyl
Palmitate)
then
behavior
under
monitored.
Finally,
were
irradiated
analyzed
spectroscopy
HT‐GC/MS
to
respectively
follow
oxidation
process
identify
When
alone,
SQ
detectable
only
after
2430
J/cm
2
dose.
Similar
observations
obtained
(after
1620
).
No
changes
observed
Palmitate).
contrast,
when
extracted
human
irradiated,
more
marked
modifications
SQ.
confirmed
antioxidant
role
by
scavenging
free
radicals
polyunsaturated
(PUFA).
Marine Drugs,
Journal Year:
2023,
Volume and Issue:
21(4), P. 204 - 204
Published: March 24, 2023
Carotenoids
and
squalene
are
important
terpenes
that
applied
in
a
wide
range
of
products
foods
cosmetics.
Thraustochytrids
might
be
used
as
alternative
production
organisms
to
improve
processes,
but
the
taxon
is
rarely
studied.
A
screening
62
strains
thraustochytrids
sensu
lato
for
their
potential
produce
carotenoids
was
performed.
phylogenetic
tree
built
based
on
18S
rRNA
gene
sequences
taxonomic
classification,
revealing
eight
different
clades
thraustochytrids.
Design
experiments
(DoE)
growth
models
identified
high
amounts
glucose
(up
60
g/L)
yeast
extract
15
factors
most
strains.
Squalene
carotenoid
studied
by
UHPLC-PDA-MS
measurements.
Cluster
analysis
composition
partially
mirrored
results,
indicating
possible
use
chemotaxonomy.
Strains
five
produced
carotenoids.
found
all
analyzed
Carotenoid
synthesis
dependent
strain,
medium
solidity.
related
Thraustochytrium
aureum
Thraustochytriidae
sp.
promising
candidates
synthesis.
closely
Schizochytrium
aggregatum
suitable
production.
striatum
good
compromise
both
molecule
groups.
Membranes,
Journal Year:
2022,
Volume and Issue:
12(10), P. 905 - 905
Published: Sept. 20, 2022
Carotenoids
are
potent
antioxidants
with
a
wide
range
of
biomedical
applications.
However,
their
delivery
into
human
cells
is
challenging
and
relatively
inefficient.
While
the
use
natural
water-soluble
carotenoproteins
capable
to
reversibly
bind
carotenoids
transfer
them
membranes
promising,
quantitative
estimation
remains
unclear.
In
present
work,
we
studied
echinenone
(ECN)
by
cyanobacterial
carotenoprotein
AnaCTDH
(C-terminal
domain
homolog
Orange
Carotenoid
Protein
from
Anabaena),
liposome
labelled
BODIPY
fluorescent
probe.
We
observed
that
addition
AnaCTDH-ECN
liposomes
led
significant
changes
in
fast-kinetic
component
fluorescence
decay
curve,
pointing
on
dipole-dipole
interactions
between
probe
ECN
within
membrane.
It
may
serve
as
an
indirect
evidence
To
study
detail,
carried
out
molecular
dynamics
modeling
localization
lipid
bilayer
calculate
its
orientation
factor.
Next,
exploited
FRET
assess
concentration
delivered
AnaCTDH.
Finally,
used
time-resolved
anisotropy
microviscosity
liposomal
membranes.
Incorporation
β-carotene
increased
membrane
while
effect
astaxanthin
mono-
diester
forms
was
less
pronounced.
At
temperatures
below
30
°C
concentration-dependent
manner,
supporting
protein-mediated
carotenoid
mechanism.
Combining
all
data,
propose
FRET-based
analysis
assessment
approaches
characterize
efficiency
