Lambertianic Acid from Platycladus orientalis Inhibits Muscle Atrophy in Dexamethasone-Induced C2C12 Muscle Atrophy Cells DOI Creative Commons

Chan Hee Cho,

Si Hyeon Chae,

Ngoc Han Le Thi

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1357 - 1357

Published: April 30, 2025

Platycladus orientalis, an evergreen tree belonging to the Cupressaceae family, has been traditionally used treat various ailments, including fever, cough, diarrhea, diuresis, cold symptoms, and gastrointestinal disorders in folk medicine. As part of our ongoing investigation aimed at discovering bioactive natural products elucidating their mechanisms action from sources, we investigated a methanol (MeOH) extract P. orientalis leaves. This led isolation identification labdane-type diterpene, lambertianic acid (LA), via column chromatography HPLC purification. The structure LA was elucidated using LC/MS NMR spectroscopic analyses, HR-ESIMS, while its absolute configuration confirmed through electronic circular dichroism (ECD) calculations. Recent studies have reported that diterpenes exhibit diverse pharmacological activities, such as anticancer, anti-inflammatory, anti-obesity, hypolipidemic effects. Notably, shown modulate adipocyte metabolism AMPK signaling; however, role skeletal muscle atrophy remains unexplored. Therefore, this study, effects on dexamethasone (Dex)-induced C2C12 myotubes. Treatment with concentrations 25 µM 50 significantly rescued myotube diameter reduced expression atrophy-related proteins, MuRF-1 atrogin-1/MAFbx, without compromising cell viability these moderate concentrations. These findings suggest derived exerts protective against atrophy, highlighting potential promising therapeutic candidate for muscle-wasting disorders.

Language: Английский

Dual Action of Pueraria montana var. lobata Extract on Myogenesis and Muscle Atrophy DOI Open Access
So Young Eun,

Chang Hoon Lee,

Yoon‐Hee Cheon

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(7), P. 1217 - 1217

Published: March 30, 2025

Background/Objectives: Muscle atrophy, defined by diminished muscle mass and function, is a notable concern associated with aging, disease, glucocorticoid treatment. Pueraria montana var. lobata extract (PMLE) demonstrates multiple bioactive properties, such as antioxidant, anti-inflammatory, metabolic regulatory activities; however, its role in atrophy has not been extensively investigated to date. This study examined how PMLE influences both cell differentiation dexamethasone (DEX)-induced degeneration focusing on the underlying molecular mechanisms. Methods: effects of myogenic DEX-induced atrophy. C2C12 myoblasts were treated (10-100 ng/mL) assessed for changes expression myogenesis-related genes activation Akt/mTOR AMPK/SIRT1/PGC-1α signaling cascades. In vivo, model was used assess mass, fiber morphology, changes. Results: promoted development increasing MyHC, MyoD, myogenin while activating protein synthesis mitochondrial biogenesis pathways. counteracted myotube restoring diameter promoting cellular fusion vitro. mitigated degradation fast-twitch groups reversed suppression key anabolic Conclusions: These findings suggest that promotes protects against regulating critical pathways, indicating promise treatment candidate conditions involving wasting. Further studies are required clinical application long-term safety efficacy.

Language: Английский

Citations

0
Oxidative stress as a catalyst in prostate cancer progression: unraveling molecular mechanisms and exploring therapeutic interventions DOI Creative Commons
Yawen Song,

Zheng Hou,

Lingqun Zhu

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 3, 2025

Language: Английский

Citations

0
Clinical study of traditional Chinese medicine comprehensive therapy for Exercise-Related musculoskeletal injuries using musculoskeletal ultrasound observation DOI Creative Commons

Lixia Yuan,

Juan Shi, Xiaoli Liu

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 5, 2025

This study aimed to compare the clinical efficacy of Jingshang Gao in treatment lateral ankle sprains by observing healing process with musculoskeletal ultrasound. We enrolled 90 patients who were admitted our hospital from July 1, 2022, 2023. The average age was 36.21 years, and 35 male (38.9%). Patients divided into two groups based on different methods: control group received oral celecoxib capsules(200 mg once daily), research topical application. compared basic data between groups. In terms pain score, both had lower VAS scores at T1-T4 than T0, significantly T3 T4 (p < 0.01, Cohen's d = 0.82). functional higher Kaikkonen injury function 0.87). AOFAS (94.307 ± 18.206 vs. 81.216 17.22, p 0.001, 0.75). Musculoskeletal ultrasound showed that rate ligament 82.2% (95% CI: 71.1-93.3%), which group's 57.8% 43.3-72.3%), 0.011. SF-36 a VT score (75.6 9.2 68.4 8.9, 0.024, 0.79), MH (60.2 7.8 65.9 8.2, 0.032, 0.71). thickness anterior talofibular calcaneofibular ligament, thinner ATFL (1.78 0.21 2.05 0.24 mm, 1.19) CFL (1.32 0.09 1.41 0.08 1.06) group. observation for has shown promising results relieving pain, improving function, promoting healing. These findings suggest potential benefits this approach, though randomized controlled trials are needed definitive assessment. Not applicable.

Language: Английский

Citations

0
Editorial: Physiology of human myopathies DOI Creative Commons
Aldrin V. Gomes, José R. Pinto, Danuta Szczesna‐Cordary

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

Language: Английский

Citations

0
Lambertianic Acid from Platycladus orientalis Inhibits Muscle Atrophy in Dexamethasone-Induced C2C12 Muscle Atrophy Cells DOI Creative Commons

Chan Hee Cho,

Si Hyeon Chae,

Ngoc Han Le Thi

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1357 - 1357

Published: April 30, 2025

Platycladus orientalis, an evergreen tree belonging to the Cupressaceae family, has been traditionally used treat various ailments, including fever, cough, diarrhea, diuresis, cold symptoms, and gastrointestinal disorders in folk medicine. As part of our ongoing investigation aimed at discovering bioactive natural products elucidating their mechanisms action from sources, we investigated a methanol (MeOH) extract P. orientalis leaves. This led isolation identification labdane-type diterpene, lambertianic acid (LA), via column chromatography HPLC purification. The structure LA was elucidated using LC/MS NMR spectroscopic analyses, HR-ESIMS, while its absolute configuration confirmed through electronic circular dichroism (ECD) calculations. Recent studies have reported that diterpenes exhibit diverse pharmacological activities, such as anticancer, anti-inflammatory, anti-obesity, hypolipidemic effects. Notably, shown modulate adipocyte metabolism AMPK signaling; however, role skeletal muscle atrophy remains unexplored. Therefore, this study, effects on dexamethasone (Dex)-induced C2C12 myotubes. Treatment with concentrations 25 µM 50 significantly rescued myotube diameter reduced expression atrophy-related proteins, MuRF-1 atrogin-1/MAFbx, without compromising cell viability these moderate concentrations. These findings suggest derived exerts protective against atrophy, highlighting potential promising therapeutic candidate for muscle-wasting disorders.

Language: Английский

Citations

0