Transcriptome analysis to identify genes related to programmed cell death resulted from manipulating of BnaFAH ortholog by CRISPR/Cas9 in Brassica napus DOI Creative Commons
Zhou Zhou,

Tiantian Zhi,

J. Zou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of tyrosine degradation pathway. In this study, we isolated and characterized two homologous BnaFAH genes in Brassica napus L. variant Westar, then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series transgene-free mutant lines either with single or double-null bnafah alleles. Among these lines, aacc (bnafah) line, rather than aaCC (bnaa06fah) exhibited programmed cell death (PCD) under short days (SD). Histochemical staining content measurement confirmed that accumulation reactive oxygen species (ROS) was significantly higher bnaa06fah. To further elucidate mechanism PCD, performed transcriptomic analyses bnaa06fah at different SD stages. A heatmap cluster differentially expressed (DEGs) revealed PCD may be related various redox regulatory involved antioxidant activity, ROS-responsive regulation calcium signaling. Combined results previous studies, our work expression levels BnaC04CAT2, BnaA09/C09SAL1, BnaA08/C08ACO2, BnaA07/C06ERO1, BnaA08ACA1, BnaC04BIK1, BnaA09CRK36 BnaA03CPK4 were might candidate hub for PCD. Together, underscore ability phenotypes alter orthologs through gene editing elucidated molecular mechanisms oxidative stress-induced plants.

Language: Английский

Lonicerin protects pancreatic acinar cells from caerulein-induced apoptosis, inflammation, and ferroptosis by activating the SIRT1/GPX4 signaling pathway DOI

Dahuan Li,

Chunyan Li,

Simin Jiang

et al.

Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 492, P. 117136 - 117136

Published: Oct. 30, 2024

Language: Английский

Citations

0
Transcriptome analysis to identify genes related to programmed cell death resulted from manipulating of BnaFAH ortholog by CRISPR/Cas9 in Brassica napus DOI Creative Commons
Zhou Zhou,

Tiantian Zhi,

J. Zou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of tyrosine degradation pathway. In this study, we isolated and characterized two homologous BnaFAH genes in Brassica napus L. variant Westar, then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series transgene-free mutant lines either with single or double-null bnafah alleles. Among these lines, aacc (bnafah) line, rather than aaCC (bnaa06fah) exhibited programmed cell death (PCD) under short days (SD). Histochemical staining content measurement confirmed that accumulation reactive oxygen species (ROS) was significantly higher bnaa06fah. To further elucidate mechanism PCD, performed transcriptomic analyses bnaa06fah at different SD stages. A heatmap cluster differentially expressed (DEGs) revealed PCD may be related various redox regulatory involved antioxidant activity, ROS-responsive regulation calcium signaling. Combined results previous studies, our work expression levels BnaC04CAT2, BnaA09/C09SAL1, BnaA08/C08ACO2, BnaA07/C06ERO1, BnaA08ACA1, BnaC04BIK1, BnaA09CRK36 BnaA03CPK4 were might candidate hub for PCD. Together, underscore ability phenotypes alter orthologs through gene editing elucidated molecular mechanisms oxidative stress-induced plants.

Language: Английский

Citations

0