Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 31, 2023

Abstract Alzheimer's disease (AD) is a neurodegenerative characterized by amyloid-beta (Aβ) aggregation, neuroinflammation, oxidative stress, and dysfunction in the mitochondria cholinergic system. In this study, synthesis of chitosan-polylactic acid-loaded magnesium oxide nanocomposite (CH/PLA/MgONCs) was examined using green precipitation method. The synthesized CH/PLA/MgONCs were confirmed UV-Vis spectrum, FT-IR, SEM-EDAX, physical properties. experiments carried out male Wistar rats injecting streptozotocin (STZ) bilaterally into brain's ventricles through intracerebroventricular (ICV) route at dose 3 mg/kg. We also evaluated effects doses 10 To assess cognitive induced ICV-STZ, we performed behavioral, biochemical, histopathological analyses. our study results, spectrum analysis showed 285 nm, FT-IR analyses that various functional groups present, SEM-EDAX cauliflower-like spherical shape, Mg O present. Treatment with (10 mg/kg) significant improvement spatial non-spatial memory functions. This further supported biochemical showing improved antioxidant enzyme (GSH, SOD, CAT, GPx activity) activities significantly attenuated activity stress. CH/PLA/MgONCs-treated group, observed mitochondrial complex activity. ICV-STZ-induced as indicated increased levels TNF-α, IL-6, CRP, reduced treatment. Additionally, treated histological results healthy neuronal cells brain. Furthermore, silico studies confirm these molecules have good binding affinity inhibit Aβ aggregation. conclusion, treatment reversed AD pathology improving reducing dysfunction. These findings recommend are possible therapeutic agents to treat AD.

Language: Английский