Morphological, Biochemical and Molecular Docking Evaluation of the Anti-inflammatory Effects of Methanolic Extract of Bridelia ferruginea stem bark on Acetic acid-induced Ulcerative Colitis in Rats DOI Creative Commons

Omolaso Blessing Oluwagbamila,

Adeoti Gbemisola Adeniran,

Kayode Adeniran

et al.

Nigerian Journal of Physiological Sciences, Journal Year: 2023, Volume and Issue: 38(1), P. 79 - 89

Published: June 30, 2023

Ulcerative colitis (UC) is a chronic disorder that involves inflammation. This study was carried out to examine the anti-inflammatory effect of methanol extract Brideliaferrugineastem barkinAcetic acid-induced ulcerative inmaleWistarrats. Twenty-four rats were randomly divided into 6 groups 4 animals each, thereafter induced by intrarectal administration of4% (v/v)Aceticacidin all except group 1, which received distilled water. For post-colitis induction treatment 2 water, 3, and 5 orally administeredthe at doses 100mg/kg, 200mg/kg 400mg/kg, respectively while sulfasalazine 500mg/kg orally.Post induction, lasted for 7 days end experiment, colon samples collected estimation antioxidant, inflammatory histological parameters. Molecular docking also gain more insights about promising compounds earlier identified in extract.Results revealed significantly (p<0.05) attenuated increased MDA, nitrite,TNF-α IL-6 levels. Activities SOD, CAT, MPO GSH levelswere also, increased. Furthermore, molecular α-amyrin may have contributed activity because its remarkable binding affinity IL-6, iNOS, IL1-β,TNF-α COX-2 relative prednisolone celecoxib. suggests acetic via antioxidative mechanisms. .

Language: Английский

Modulation of Multidrug Resistance Protein 1-mediated Transport Processes by the Antiviral Drug Ritonavir in Cultured Primary Astrocytes DOI Creative Commons
Christian Arend, Isabell Louise Grothaus, Mario Waespy

et al.

Neurochemical Research, Journal Year: 2023, Volume and Issue: 49(1), P. 66 - 84

Published: Aug. 21, 2023

Abstract The Multidrug Resistance Protein 1 (Mrp1) is an ATP-dependent efflux transporter and a major facilitator of drug resistance in mammalian cells during cancer HIV therapy. In brain, Mrp1-mediated GSH export from astrocytes the first step supply precursors to neurons. To reveal potential mechanisms underlying drug-induced modulation transport processes, we investigated effects antiviral ritonavir on cultured rat primary astrocytes. Ritonavir strongly stimulated glutathione (GSH) by decreasing K m value 200 nmol/mg 28 nmol/mg. contrast, decreased other Mrp1 substrates disulfide (GSSG) bimane-glutathione. give explanation for these apparently contradictory observations, performed silico docking analysis molecular dynamics simulations using homology model predict binding modes ritonavir, GSSG Mrp1. results suggest that binds hydrophilic part bipartite site thereby differently affects substrates. These new insights into processes provide better understand GSH-dependent detoxification brain cells.

Language: Английский

Citations

6
Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review DOI Creative Commons

Bo Chen,

Xinqian Dong,

Jinlong Zhang

et al.

Medicine, Journal Year: 2023, Volume and Issue: 102(46), P. e36157 - e36157

Published: Nov. 17, 2023

Gastric cancer (GC) is a global public health concern that poses serious threat to human owing its high morbidity and mortality rates. Due the lack of specificity symptoms, patients with GC tend be diagnosed at an advanced stage poor prognosis. Therefore, development new treatment methods particularly urgent. Chronic atrophic gastritis (CAG), precancerous lesion, plays key role in occurrence development. Oxidative stress has been identified as important factor driving progression pathological processes CAG GC. regulating oxidative pathways can not only intervene but also prevent metastasis improve prognosis patients. In this study, PubMed, CNKI, Web Science were used search for large number relevant studies. The review results suggested active ingredients traditional Chinese medicine (TCM) TCM prescriptions could target inflammation, status, metastasis, invasion tumor cells, providing potential supplement

Language: Английский

Citations

6
Developmental Expression of Drug Transporters and Conjugating Enzymes Involved in Enterohepatic Recycling: Implication for Pediatric Drug Dosing DOI
Aarzoo Thakur, Sandhya Subash, Deepak Ahire

et al.

Clinical Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: 116(6), P. 1615 - 1626

Published: Aug. 19, 2024

Around 50% of the drugs used in children have never been tested for safety and efficacy this vulnerable population. Immature drug elimination pathways can lead to toxicity when pediatric doses are determined using empirical methods such as body‐surface area or body‐weight‐normalized adult dosing. In absence clinical data, physiologically‐based pharmacokinetic (PBPK) modeling has emerged a useful tool predict pharmacokinetics children. These models utilize developmental physiological including age‐dependent differences abundance drug‐metabolizing enzymes transporters (DMET), mechanistically extrapolate data The reported hepatic DMET proteins subcellular fractions isolated from frozen tissue prone high technical variability. Therefore, we carried out proteomics‐based quantification conjugating 50 8 human hepatocyte samples. Out 34 studied proteins, 28 showed significant increase decrease with age. While MRP6, OAT7, SULT1E1 were highest < 1‐year‐old samples, P‐gp UGT1A4 was negligible samples increased significantly after 1 year Incorporation PBPK help improve dose prediction, leading safer pharmacotherapy

Language: Английский

Citations

1
In vitro galactose impairs energy metabolism in the brain of young rats: protective role of antioxidants DOI
Daniela Delwing de Lima,

Simone Sasso,

Débora Delwing Dal Magro

et al.

Nucleosides Nucleotides & Nucleic Acids, Journal Year: 2023, Volume and Issue: 42(12), P. 967 - 985

Published: June 15, 2023

We, herein, investigated the in vitro effects of galactose on activity pyruvate kinase, succinate dehydrogenase (SDH), complex II and IV (cytochrome c oxidase) respiratory chain Na+K+-ATPase cerebral cortex, cerebellum hippocampus 30-day-old rats. We also determined influence antioxidants, trolox, ascorbic acid glutathione, elicited by galactose. Galactose was added to assay at concentrations 0.1, 3.0, 5.0 10.0 mM. Control experiments were performed without Galactose, mM, decreased kinase cortex mM hippocampus. reduced SDH activities hippocampus, cytochrome oxidase Additionally, hippocampus; conversely, galactose, 3.0 increased this enzyme's cerebellum. Data show that disrupts energy metabolism glutathione addition prevented majority alterations parameters analyzed, suggesting use antioxidants as an adjuvant therapy Classic galactosemia.

Language: Английский

Citations

2
Morphological, Biochemical and Molecular Docking Evaluation of the Anti-inflammatory Effects of Methanolic Extract of Bridelia ferruginea stem bark on Acetic acid-induced Ulcerative Colitis in Rats DOI Creative Commons

Omolaso Blessing Oluwagbamila,

Adeoti Gbemisola Adeniran,

Kayode Adeniran

et al.

Nigerian Journal of Physiological Sciences, Journal Year: 2023, Volume and Issue: 38(1), P. 79 - 89

Published: June 30, 2023

Ulcerative colitis (UC) is a chronic disorder that involves inflammation. This study was carried out to examine the anti-inflammatory effect of methanol extract Brideliaferrugineastem barkinAcetic acid-induced ulcerative inmaleWistarrats. Twenty-four rats were randomly divided into 6 groups 4 animals each, thereafter induced by intrarectal administration of4% (v/v)Aceticacidin all except group 1, which received distilled water. For post-colitis induction treatment 2 water, 3, and 5 orally administeredthe at doses 100mg/kg, 200mg/kg 400mg/kg, respectively while sulfasalazine 500mg/kg orally.Post induction, lasted for 7 days end experiment, colon samples collected estimation antioxidant, inflammatory histological parameters. Molecular docking also gain more insights about promising compounds earlier identified in extract.Results revealed significantly (p<0.05) attenuated increased MDA, nitrite,TNF-α IL-6 levels. Activities SOD, CAT, MPO GSH levelswere also, increased. Furthermore, molecular α-amyrin may have contributed activity because its remarkable binding affinity IL-6, iNOS, IL1-β,TNF-α COX-2 relative prednisolone celecoxib. suggests acetic via antioxidative mechanisms. .

Language: Английский

Citations

2