The cardio‐metabolic protein profile is associated with development of type 2 diabetes at long term follow‐up after gestational diabetes mellitus: Results from the OGFUS study

Diabetes Obesity and Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Aims Women with previous gestational diabetes mellitus (GDM) have an increased risk for later development of type 2 diabetes. During pregnancy, GDM affects the cardio‐metabolic protein profile; however, it is unknown how profile in long term and if associated after GDM. We hypothesise that affected Materials Methods A case–control follow‐up study based on Odense Follow‐Up Study (OGFUS) cohort, which included women ( n = 128) matched controls without 70). Blood samples from a assessment 8–10 years delivery were analysed using 29‐plex panel apolipoproteins, transport inflammation/immune proteins multiple‐reaction‐monitoring mass spectrometry. Results Apolipoprotein A‐I, D M significantly lower compared to (all p < 0.001), while apolipoprotein L‐I, H, vitamin binding protein, CRP, vitronectin, transthyretin complement factors 3 B higher 0.008, 0.001, 0.005, respectively). All associations remained significant adjustment multiple testing except whereas D, vitronectin 0.02, 0.004, Conclusions The pregnancy altered are candidate markers pregnancy.

Language: Английский

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Apolipoprotein profile in early pregnancy and the link to gestational diabetes mellitus: Insights from Odense Child Cohort

Endocrine Practice, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

We aimed to investigate potential associations between serum apolipoprotein levels in early pregnancy and the risk of gestational diabetes mellitus (GDM) adverse outcomes. This was an observational study population-based Odense Child Cohort. Pregnant women were followed from inclusion until childbirth. Apolipoprotein levels, including 12 apolipoproteins (ApoA-I, ApoA-II, ApoA-IV, ApoB, ApoC-I, ApoC-II, ApoC-III, ApoD, ApoE, ApoH, ApoJ, ApoM) measured by targeted proteomics using liquid chromatography mass spectrometry on late first trimester samples stored a biobank. GDM defined WHO 2013 diagnostic criteria. A total 991 pregnant included, which 415 (41.9%) diagnosed with GDM. associated increasing ApoB (adjusted OR: 1.26, P = 0.002) ApoD 0.84, 0.021). correlated significantly positively insulin resistance (r 0.22, < 0.001) beta-cell function 3rd 0.20, 0.001), whereas inversely -0.14, -0.12, 0.001). Finally, high lower large-for-gestational-age (LGA) infants 0.78, 0.041). High low independently increased GDM, LGA (low only), suggesting roles for future management

Language: Английский

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Chondroprotective effects of Apolipoprotein D in knee osteoarthritis mice through the PI3K/AKT/mTOR signaling pathway

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 133, P. 112005 - 112005

Published: April 15, 2024

Because the pathophysiology of osteoarthritis (OA) has not been fully elucidated, targeted treatments are lacking. In this study, we assessed role and underlying mechanism apolipoprotein D (APOD) on development OA.

Language: Английский

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Jinwu Jiangu capsule attenuates rheumatoid arthritis via the SLC7A11/GSH/GPX4 pathway in M1 macrophages

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156232 - 156232

Published: Nov. 15, 2024

JinWu JianGu capsule (JWJGC) is a Chinese herbal medicine that alleviates the clinical manifestations of rheumatoid arthritis (RA). However, mechanism action requires further investigation..

Language: Английский

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Modeling the acutely injured brain environment in vitro.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

ABSTRACT A major challenge to study the regenerative potential of injured brain is limited access this organ in vivo . To address this, we developed an innovative gliosis model that, although established vitro , originates from a genuine injury The relies on reactive glia acquiring enhanced adhesion, facilitating their rapid adaptation conditions, where it faithfully recapitulates key features injury. These include secretome associated with pathways, degenerative responses like neuronal death and neuroinflammation, processes such as progenitor proliferation, recruitment commitment oligodendrocytes. Moreover, exposure adult glial cells culture medium acquisition multipotency observed both mouse human brains. Finally, our approach allows studying glia-to-neuron reprogramming, process challenging tackle Consequently, present novel tool for exploring stem cell dynamics behaviors CNS pathology.

Language: Английский

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A Golgi apparatus‑related subtype and risk signature predicts prognosis and evaluates immunotherapy response in gastric cancer

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 22, 2025

Gastric cancer (GC) remains a significant health burden, calling for the discovery of novel biomarkers. Golgi apparatus, crucial cellular organelle involved in tumorigenesis, underexplored GC research. A comprehensive understanding its role and associated mechanisms is urgently needed. Utilizing TCGA-STAD dataset as training cohort GSE84433 validation cohort, we explored potential associations between apparatus-related genes (GARG) clinical risk. We aimed to decipher prognostic significance underlying biological these via consistent clustering, differential expression analysis, enrichment analyses, immune infiltration profiling. To assess relationship risk stratification survival outcomes, drug sensitivity, infiltration, developed Apparatus-Related Risk Signature (GARRS). The reliability GARRS was further corroborated using immunohistochemical staining. Consensus clustering based on 17 GARG identified two patient subgroups, C1 C2, exhibiting survival, scores, cell infiltration. Cox-Lasso regression accurately stratifying patients into high- low-risk groups. GARRS' validity confirmed set Our findings underline apparatus' microenvironment utility predicting outcomes. This study underscores association apparatus subtypes immunotumor microenvironment. GARRS, validated prognostic, sensitivity predictive abilities, offers new insights gastric treatment strategies.

Language: Английский

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Elaboration and verification of immune-based diagnostic biomarker panel for diabetic foot ulcer

Journal of Diabetes and its Complications, Journal Year: 2025, Volume and Issue: unknown, P. 108957 - 108957

Published: Jan. 1, 2025

Language: Английский

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Evaluating the causal effects of circulating metabolic biomarkers on Alzheimer's disease

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Journal Year: 2025, Volume and Issue: 138, P. 111309 - 111309

Published: March 2, 2025

Language: Английский

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Apolipoprotein and sphingolipid measurements: Can be used in the clinical practice of atrial fibrillation diagnosing and evaluating the cryoablation effectiveness?

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0315905 - e0315905

Published: March 4, 2025

Atrial fibrillation (AF) has become the most common arrhythmia of clinical importance. A well-established and recommended therapeutic option for AF is balloon-based cryoablation (CBA) method. There are still no sensitive biomarkers prediction effectiveness assessment, therefore in our prospective study, we examined plasma content apolipoproteins (Apo) sphingolipids, as well distribution selected sphingolipids among lipoprotein fractions. The study included 33 patients with on admission 24 h after therapy, while 20 healthy volunteers were recruited to control group. Plasma Apo concentrations determined using a multiplex assay kit measuring fluorescence signal, whereas high-performance liquid chromatography (HPLC) method was applied assess total sphingolipid levels their isolated Our results showed that cryoballoon ablation markedly reduced level almost all compared individuals from Pre-CBA groups (Apo-A1: -25.9% -20.0%, Apo-A2: -19.9% -17.3%, Apo-B: -26.8% -14.4%, Apo-C1: -20.3% -13.4%, Apo-D: -15.9% -22.2%, Apo-E: -18.3% -14.3%, Apo-J: -36.4% -21.5%, p < 0.05, respectively). Importantly, area under curve Apo-J (AUC 0.81; 95% CI, 0.71-0.92) indicates it might be useful biomarker cryotherapy success patients. Moreover, also observed pronounced increase sphinganine (Sa; +33.5%), sphingosine (So; +24.6%), sphinganine-1-phosphate (Sa1P; +34.3%), sphingosine-1-phosphate (So1P; +22.3%) group comparison controls. This first evaluates such broad panel undergoing CBA procedure, however, confirm whether any these parameters could clinically predicting or assessing treatment, further research will necessary due limitations study.

Language: Английский

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Apolipoproteins in ischemic stroke progression and recovery: Key molecular mechanisms and therapeutic potential

Neurobiology of Disease, Journal Year: 2025, Volume and Issue: unknown, P. 106896 - 106896

Published: April 1, 2025

Ischemic stroke, responsible for 80 % of all strokes, is a leading cause mortality globally. While altered lipids profiles are recognized as modifiable risk factors, their direct impact on stroke outcomes less understood due to the brain's distinct lipid metabolism and selective permeability blood-brain barrier lipoproteins. As key components lipoproteins, apolipoproteins essential transport, redistribution in both central nervous system peripheral blood circulation. This review provides an updated perspective influence brain-expressed (such ApoE, ApoA-I, ApoJ, ApoD, others) those that cross damaged following ischemic stroke. We explore hypotheses regarding involvement molecular pathways related metabolism, inflammation, oxidative stress, mitochondrial function integrity. Through this synthesis, we aim identify potential biomarkers therapeutic targets, thereby enhancing our understanding progression contributing improved clinical outcomes.

Language: Английский

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