Berries and Their Active Compounds in Prevention of Age-Related Macular Degeneration DOI Creative Commons
Xiang Li, Li Zhao, Bowei Zhang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1558 - 1558

Published: Dec. 18, 2024

Age-related macular degeneration (AMD) is a leading cause of vision loss in the elderly, significantly diminishing quality life. Currently, there no available treatment to reverse retinal and neuronal loss, prompting focus on interventions that slow progression intermediate AMD geographic atrophy. Berries are rich bioactive compounds, including flavonoids, anthocyanins, carotenoids, resveratrol, known for their antioxidant, anti-inflammatory, anti-angiogenic properties. Preclinical studies suggest extracts from various berries, such as aronia, honeysuckle, black currant, goji, bilberry, can improve health by reducing oxidative stress inflammation. Although clinical trials limited, emerging evidence indicates dietary intake these compounds may enhance visual function AMD. This review summarizes findings both animal identify specific berries have been validated prevent or delay progression, well those with potential therapeutic value. Furthermore, we examine key phytochemicals present mechanisms action degeneration, distinct properties application. A deeper understanding characteristics could enable rational appliance especially wolfberry, berry-derived components, carotenoids optimize better outcomes management.

Language: Английский

Gengnianchun Against H2O2‐Induced Oxidative Damage in KGN Cells via miR‐548m/FOXO3 Signaling DOI Open Access

Jun Li,

Yanqiu Rao, Tao Pu

et al.

Journal of Cellular Biochemistry, Journal Year: 2025, Volume and Issue: 126(1)

Published: Jan. 1, 2025

ABSTRACT Gengnianchun (GNC) is a traditional remedy used for diminished ovarian reserve, but its underlying mechanisms remain unclear. This study aimed to explore these in human granulosa‐like cancer (KGN) cells pretreated with medicated rat serum (MRS) before H 2 O exposure. MRS pretreatment significantly alleviated ‐induced cell damage, including improvements viability, superoxide dismutase and GSH‐Px activities, Bcl‐2 expression. Conversely, treatment increased apoptosis, autophagosomes, IL‐1β, TNF‐α, reactive oxygen species, malondialdehyde levels, the expression of LC‐II/LC3‐I, Bax, Beclin‐1. GEO database analysis revealed significant differential several miRNAs, miR‐548m. qPCR confirmed that upregulated miR‐548m expression, which was downregulated by dose‐dependent manner. Preincubation prevented decline mitigated autophagy, oxidative stress. suppressed FOXO3 3'‐UTR luciferase activity, anti‐miR‐548m enhanced it. Transfection reduced mRNA protein while them. These findings suggest GNC protects against damage modulating miR‐548m/FOXO3 axis, triggering autophagy apoptosis.

Language: Английский

Citations

0
Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study DOI Creative Commons
Liang Tang, Xinyu Fan, Yongqing Xu

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

ABSTRACT Luteolin (LUT) has been suggested as an inhibitor of osteoporosis (OP). This investigation examines the pivotal role miR‐125b‐5p/SIRT3/AMPK/mTOR pathway in mediating luteolin‐induced effects on OP. Mesenchymal stem cells derived from bone marrow (BMSCs) were exposed to dexamethasone (DEX) create vitro model Following treatment with luteolin, levels miR‐125b‐5p and SIRT3 quantified using reverse transcription polymerase chain reaction. Moreover, SIRT3, AMPK, mTOR protein levels, osteogenesis (OPN, Runx2, OSX, OCN), autophagy (p62, ATG5, LC3, BECN1) evaluated ELISA. Additionally, specific mimics siRNA constructed overexpress or downregulate SIRT3. Furthermore, animal models DEX‐induced OP assess LUT at doses 50 100 mg/kg/day histology, stereology, biochemistry, expression miR‐125b‐5p, SIRT3/AMPK/mTOR axis, markers autophagy. The findings revealed that suppressed expression, overexpressed downregulated BMSCs compared DEX ( p ‐value < 0.01). Interestingly, restored for 0.001). overexpression downregulation inhibited therapeutic properties. In animals, improved histology 0.05) while overexpressing AMPK RUNX2, OPN, OCN improved, was enhanced LUT‐treated rats. current could promote improve via activation through pathway.

Language: Английский

Citations

0
Potential of autophagy in subretinal fibrosis in neovascular age-related macular degeneration DOI Creative Commons
Janusz Błasiak, Elżbieta Pawłowska, Hanna Heloterä

et al.

Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 30, 2025

Language: Английский

Citations

0
Heat-killed probiotic Levilactobacillus brevis MKAK9 and its exopolysaccharide promote longevity by modulating aging hallmarks and enhancing immune responses in Caenorhabditis elegans DOI Creative Commons
Arun Kumar,

Manti Kumar Saha,

Vipin Kumar

et al.

Immunity & Ageing, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 2, 2024

Proteostasis is a critical aging hallmark responsible for removing damaged or misfolded proteins and their aggregates by improving proteasomal degradation through the autophagy-lysosome pathway (ALP) ubiquitin-proteasome system (UPS). Research on impact of heat-killed probiotic bacteria structural components hallmarks innate immune responses scarce, yet enhancing these effects could potentially delay age-related diseases.

Language: Английский

Citations

3
Lutein Modulates Cellular Functionalities and Regulates NLRP3 Inflammasome in a H2O2-Challenged Three-Dimensional Retinal Pigment Epithelium Model DOI
Hang Liu,

Chushan Wu,

Shiyin Hu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(26), P. 14701 - 14712

Published: June 19, 2024

Excessive hydrogen peroxide (H

Language: Английский

Citations

2
The Novel Application of EUK‐134 in Retinal Degeneration: Preventing Mitochondrial Oxidative Stress‐Triggered Retinal Pigment Epithelial Cell Apoptosis by Suppressing MAPK/p53 Signaling Pathway DOI

Shang‐Chun Tsou,

Chen‐Ju Chuang, Chin‐Lin Hsu

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

ABSTRACT Age‐related macular degeneration (AMD), a leading cause of blindness, is characterized by mitochondrial dysfunction retinal pigment epithelium (RPE) cells. EUK‐134 mimetic SOD2 and catalase, widely used for its antioxidant properties in models light‐induced damage or oxidative stress. However, effects on the retina are not yet clear. Here, we investigated capability averting AMD using sodium iodate (NaIO 3 )‐induced Balb/c mouse ARPE‐19 cells (adult RPE cell line). In vivo, effectively antagonized NaIO ‐induced deformation prevented outer inner nuclear layer thinning. addition, it was found that EUK‐134‐treated group significantly down‐regulated expression cleaved caspase‐3 compared with treated alone. Our results notably improved viability preventing ROS accumulation‐induced membrane potential depolarization‐mediated apoptosis ‐inducted Furthermore, could inhibit p‐ERK, p‐p38, p‐JNK, p‐p53, Bax, caspase‐9, caspase‐3, PARP increasing Bcl‐2 protein expression. Additionally, employed MAPK pathway inhibitors SB203580 (a p38 inhibitor), U0126 (an ERK SP600125 JNK inhibitor) to corroborate aforementioned observation. The support may prevent stress‐mediated retinopathy.

Language: Английский

Citations

2
Plant bioactive compounds alleviate photoinduced retinal damage and asthenopia: Mechanisms, synergies, and bioavailability DOI
Huijuan Zhang,

Tiancong Song,

Rui Kang

et al.

Nutrition Research, Journal Year: 2023, Volume and Issue: 120, P. 115 - 134

Published: Oct. 29, 2023

Language: Английский

Citations

4
Heat-killed probiotic Levilactobacillus brevis MKAK9 and its exopolysaccharide promote longevity by modulating aging hallmarks and enhancing immune responses in Caenorhabditis elegans DOI
Arun Kumar,

Manti Kumar Saha,

Vipin Kumar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 9, 2024

Abstract Background Proteostasis is a critical aging hallmark responsible for removing damaged or misfolded proteins and their aggregates by improving proteasomal degradation through the autophagy-lysosome pathway (ALP) ubiquitin-proteasome system (UPS). Research on impact of heat-killed probiotic bacteria structural components hallmarks innate immune responses scarce, yet enhancing these effects could potentially delay age- related diseases. Results This study introduces novel Levilactobacillus brevis strain MKAK9 (HK MKAK9), along with its exopolysaccharide (EPS), demonstrating ability to extend longevity proteostasis in wild-type Caenorhabditis elegans . We elucidate underlying mechanisms comprehensive approach involving mRNA- small RNA sequencing, proteomic analysis, lifespan assays loss-of- function mutants, quantitative RT-PCR. Mechanistically, HK EPS resulted downregulation insulin-like signaling DAF-16-dependent manner, protein ubiquitination subsequent activation ALP pathway, which partially mediated microRNA mir-243. Importantly, autophagosomes engulf ubiquitinylated proteins, as evidenced increased expression autophagy receptor sqst-3 , subsequently fuse lysosomes, facilitated levels lysosome-associated membrane (LAMP) lmp-1, suggesting formation autolysosomes selected cargo. Moreover, activated p38 MAPK downstream SKN-1 transcription factor, are known regulate genes involved response ( thn -1, ilys cnc-2 spp-9 spp -21, clec -47, -266) antioxidation sod-3 gst-44 ), thereby reducing accumulation reactive oxygen species (ROS) at both cellular mitochondrial levels. Notably, SOD-3 emerged transcriptional target DAF-16 factors. Conclusion Our research sets benchmark future investigations that specific component, EPS, can downregulate insulin- degrading promoting organismal longevity. Additionally, we discovered mir-243 regulates pathway. findings also indicate postbiotic treatment may bolster antioxidative responses, offering promising avenue interventions aging-related

Language: Английский

Citations

0
Berries and Their Active Compounds in Prevention of Age-Related Macular Degeneration DOI Creative Commons
Xiang Li, Li Zhao, Bowei Zhang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1558 - 1558

Published: Dec. 18, 2024

Age-related macular degeneration (AMD) is a leading cause of vision loss in the elderly, significantly diminishing quality life. Currently, there no available treatment to reverse retinal and neuronal loss, prompting focus on interventions that slow progression intermediate AMD geographic atrophy. Berries are rich bioactive compounds, including flavonoids, anthocyanins, carotenoids, resveratrol, known for their antioxidant, anti-inflammatory, anti-angiogenic properties. Preclinical studies suggest extracts from various berries, such as aronia, honeysuckle, black currant, goji, bilberry, can improve health by reducing oxidative stress inflammation. Although clinical trials limited, emerging evidence indicates dietary intake these compounds may enhance visual function AMD. This review summarizes findings both animal identify specific berries have been validated prevent or delay progression, well those with potential therapeutic value. Furthermore, we examine key phytochemicals present mechanisms action degeneration, distinct properties application. A deeper understanding characteristics could enable rational appliance especially wolfberry, berry-derived components, carotenoids optimize better outcomes management.

Language: Английский

Citations

0